Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral pha...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2003-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2003.30 |
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| Summary: | The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix. |
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| ISSN: | 1537-744X |