Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i>
<i>Listeria monocytogenes</i> represents one of the main risks for food safety worldwide. Two enzyme-based antimicrobials (enzybiotics) have been combined in a novel treatment against this pathogenic bacterium, resulting in a powerful synergistic effect. One of the enzymes is an endolysi...
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2024-12-01
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| author | David Talens-Perales José-Antonio Daròs Julio Polaina Julia Marín-Navarro |
| author_facet | David Talens-Perales José-Antonio Daròs Julio Polaina Julia Marín-Navarro |
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| description | <i>Listeria monocytogenes</i> represents one of the main risks for food safety worldwide. Two enzyme-based antimicrobials (enzybiotics) have been combined in a novel treatment against this pathogenic bacterium, resulting in a powerful synergistic effect. One of the enzymes is an endolysin from <i>Listeria</i> phage vB_LmoS_188 with amidase activity (henceforth A10), and the other is an engineered version of glucose oxidase from <i>Aspergillus niger</i> (GOX). Both enzymes, assayed separately against <i>Listeria innocua</i>, showed antibacterial activity at the appropriate doses. The combination of the two enzybiotics resulted in a synergistic effect with a log reduction in viable cells (log N<sub>0</sub>/N) of 4, whereas, taken separately, the same dose of A10 and GOX caused only 1.2 and 0.2 log reductions, respectively. Flow cytometry and microscopy analyses revealed that A10 treatment alone induced the aggregation of dead cells. <i>L. monocytogenes</i> showed higher resistance to single treatment with GOX or A10 than <i>L. innocua</i>. However, the synergic combination of A10 and GOX resulted in a high lethality of <i>L. monocytogenes</i> with a log N<sub>0</sub>/N higher than 5 (below the detection limit in our analysis). Altogether, these results represent a novel efficient and eco-friendly antimicrobial treatment against the most lethal food-borne pathogen. |
| format | Article |
| id | doaj-art-3792e6de27e84c03995efda363a98d1a |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Biomolecules |
| spelling | doaj-art-3792e6de27e84c03995efda363a98d1a2025-01-24T13:24:53ZengMDPI AGBiomolecules2218-273X2024-12-011512410.3390/biom15010024Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i>David Talens-Perales0José-Antonio Daròs1Julio Polaina2Julia Marín-Navarro3Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), 46980 Paterna, Valencia, SpainInstituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universitat Politècnica de València, 46022 Valencia, Valencia, SpainInstituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), 46980 Paterna, Valencia, SpainInstituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), 46980 Paterna, Valencia, Spain<i>Listeria monocytogenes</i> represents one of the main risks for food safety worldwide. Two enzyme-based antimicrobials (enzybiotics) have been combined in a novel treatment against this pathogenic bacterium, resulting in a powerful synergistic effect. One of the enzymes is an endolysin from <i>Listeria</i> phage vB_LmoS_188 with amidase activity (henceforth A10), and the other is an engineered version of glucose oxidase from <i>Aspergillus niger</i> (GOX). Both enzymes, assayed separately against <i>Listeria innocua</i>, showed antibacterial activity at the appropriate doses. The combination of the two enzybiotics resulted in a synergistic effect with a log reduction in viable cells (log N<sub>0</sub>/N) of 4, whereas, taken separately, the same dose of A10 and GOX caused only 1.2 and 0.2 log reductions, respectively. Flow cytometry and microscopy analyses revealed that A10 treatment alone induced the aggregation of dead cells. <i>L. monocytogenes</i> showed higher resistance to single treatment with GOX or A10 than <i>L. innocua</i>. However, the synergic combination of A10 and GOX resulted in a high lethality of <i>L. monocytogenes</i> with a log N<sub>0</sub>/N higher than 5 (below the detection limit in our analysis). Altogether, these results represent a novel efficient and eco-friendly antimicrobial treatment against the most lethal food-borne pathogen.https://www.mdpi.com/2218-273X/15/1/24amidasecell wallhydrogen peroxideantimicrobialantibacterialfood safety |
| spellingShingle | David Talens-Perales José-Antonio Daròs Julio Polaina Julia Marín-Navarro Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i> Biomolecules amidase cell wall hydrogen peroxide antimicrobial antibacterial food safety |
| title | Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i> |
| title_full | Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i> |
| title_fullStr | Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i> |
| title_full_unstemmed | Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i> |
| title_short | Synergistic Enzybiotic Effect of a Bacteriophage Endolysin and an Engineered Glucose Oxidase Against <i>Listeria</i> |
| title_sort | synergistic enzybiotic effect of a bacteriophage endolysin and an engineered glucose oxidase against i listeria i |
| topic | amidase cell wall hydrogen peroxide antimicrobial antibacterial food safety |
| url | https://www.mdpi.com/2218-273X/15/1/24 |
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