Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum
The goal of this study was to see how well Lactobacillus ruteri probiotic bacteria might infect Cryptosporidium parvum viability at various bacterial dilutions and statuses. Cryptosporidium was obtained from stool samples of diarrheal patients and diagnosed using parasitological methods, followed b...
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| Language: | English |
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middle technical university
2022-09-01
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| Series: | Journal of Techniques |
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| Online Access: | https://journal.mtu.edu.iq/index.php/MTU/article/view/551 |
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| author | Amal Jabbar Ghaib Amani Mohammed Jasim Amal Aziz Kareem |
| author_facet | Amal Jabbar Ghaib Amani Mohammed Jasim Amal Aziz Kareem |
| author_sort | Amal Jabbar Ghaib |
| collection | DOAJ |
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The goal of this study was to see how well Lactobacillus ruteri probiotic bacteria might infect Cryptosporidium parvum viability at various bacterial dilutions and statuses. Cryptosporidium was obtained from stool samples of diarrheal patients and diagnosed using parasitological methods, followed by molecular methods and cell line cultivation (Human adenocarcinoma cell). The treatment of Cryptosporidium Parvum by adding Lactobacillus ruteri dilutions and status were then incubated at incubation periods (24, 48, and 72 hr), detection of Cryptosporidium count was carried out microscopically, and the parasite's viability was determined by 3-(4,5 dimethylthiazol-2-yl)-2- Diphenyltetrazolium bromide(Mtt) technique. Lactobacillus ruteri can treat Cryptosporidium parvum by suppressing activity and lowering the count with extremely significant differences, according to the findings.
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| format | Article |
| id | doaj-art-376003251b2a4ee3993818f6b6857ded |
| institution | Kabale University |
| issn | 1818-653X 2708-8383 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | middle technical university |
| record_format | Article |
| series | Journal of Techniques |
| spelling | doaj-art-376003251b2a4ee3993818f6b6857ded2025-01-19T11:07:11Zengmiddle technical universityJournal of Techniques1818-653X2708-83832022-09-014310.51173/jt.v4i3.551Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium ParvumAmal Jabbar Ghaib0Amani Mohammed Jasim1Amal Aziz Kareem2Department of microbiology in the ministry of health/ Baghdad, IraqCollege of Health & Medical Technology - Baghdad, Middle Technical University, Baghdad, IraqCollege of Health & Medical Technology - Baghdad, Middle Technical University, Baghdad, Iraq The goal of this study was to see how well Lactobacillus ruteri probiotic bacteria might infect Cryptosporidium parvum viability at various bacterial dilutions and statuses. Cryptosporidium was obtained from stool samples of diarrheal patients and diagnosed using parasitological methods, followed by molecular methods and cell line cultivation (Human adenocarcinoma cell). The treatment of Cryptosporidium Parvum by adding Lactobacillus ruteri dilutions and status were then incubated at incubation periods (24, 48, and 72 hr), detection of Cryptosporidium count was carried out microscopically, and the parasite's viability was determined by 3-(4,5 dimethylthiazol-2-yl)-2- Diphenyltetrazolium bromide(Mtt) technique. Lactobacillus ruteri can treat Cryptosporidium parvum by suppressing activity and lowering the count with extremely significant differences, according to the findings. https://journal.mtu.edu.iq/index.php/MTU/article/view/551CryptosporidiumLactobacillus ruteriProbiotic bacteriaMTT test |
| spellingShingle | Amal Jabbar Ghaib Amani Mohammed Jasim Amal Aziz Kareem Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum Journal of Techniques Cryptosporidium Lactobacillus ruteri Probiotic bacteria MTT test |
| title | Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum |
| title_full | Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum |
| title_fullStr | Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum |
| title_full_unstemmed | Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum |
| title_short | Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum |
| title_sort | studying the effect of human lactobacillus ruteri on the viability of cryptosporidium parvum |
| topic | Cryptosporidium Lactobacillus ruteri Probiotic bacteria MTT test |
| url | https://journal.mtu.edu.iq/index.php/MTU/article/view/551 |
| work_keys_str_mv | AT amaljabbarghaib studyingtheeffectofhumanlactobacillusruteriontheviabilityofcryptosporidiumparvum AT amanimohammedjasim studyingtheeffectofhumanlactobacillusruteriontheviabilityofcryptosporidiumparvum AT amalazizkareem studyingtheeffectofhumanlactobacillusruteriontheviabilityofcryptosporidiumparvum |