Component library creation and pixel array generation with micromilled droplet microfluidics
Abstract Droplet microfluidics enable high-throughput screening, sequencing, and formulation of biological and chemical systems at the microscale. Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane (PDMS). However, developing design masks for PDMS devices can be a s...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-01-01
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| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-024-00839-6 |
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| _version_ | 1832594610276270080 |
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| author | David McIntyre Diana Arguijo Kaede Kawata Douglas Densmore |
| author_facet | David McIntyre Diana Arguijo Kaede Kawata Douglas Densmore |
| author_sort | David McIntyre |
| collection | DOAJ |
| description | Abstract Droplet microfluidics enable high-throughput screening, sequencing, and formulation of biological and chemical systems at the microscale. Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane (PDMS). However, developing design masks for PDMS devices can be a slow and expensive process, requiring an internal cleanroom facility or using an external vendor. Here, we present the first complete droplet-based component library using low-cost rapid prototyping and electrode integration. This fabrication method for droplet microfluidic devices costs less than $12 per device and a full design-build-test cycle can be completed within a day. Discrete microfluidic components for droplet generation, re-injection, picoinjection, anchoring, fluorescence sensing, and sorting were built and characterized. These devices are biocompatible, low-cost, and high-throughput. To show its ability to perform multistep workflows, these components were used to assemble droplet “pixel" arrays, where droplets were generated, sensed, sorted, and anchored onto a grid to produce images. |
| format | Article |
| id | doaj-art-375f56ba55544eab93c06fb3f14cfa40 |
| institution | Kabale University |
| issn | 2055-7434 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj-art-375f56ba55544eab93c06fb3f14cfa402025-01-19T12:27:04ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342025-01-0111111110.1038/s41378-024-00839-6Component library creation and pixel array generation with micromilled droplet microfluidicsDavid McIntyre0Diana Arguijo1Kaede Kawata2Douglas Densmore3Biomedical Engineering Department, Boston UniversityBiomedical Engineering Department, Boston UniversityBiological Design Center, Boston UniversityBiological Design Center, Boston UniversityAbstract Droplet microfluidics enable high-throughput screening, sequencing, and formulation of biological and chemical systems at the microscale. Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane (PDMS). However, developing design masks for PDMS devices can be a slow and expensive process, requiring an internal cleanroom facility or using an external vendor. Here, we present the first complete droplet-based component library using low-cost rapid prototyping and electrode integration. This fabrication method for droplet microfluidic devices costs less than $12 per device and a full design-build-test cycle can be completed within a day. Discrete microfluidic components for droplet generation, re-injection, picoinjection, anchoring, fluorescence sensing, and sorting were built and characterized. These devices are biocompatible, low-cost, and high-throughput. To show its ability to perform multistep workflows, these components were used to assemble droplet “pixel" arrays, where droplets were generated, sensed, sorted, and anchored onto a grid to produce images.https://doi.org/10.1038/s41378-024-00839-6 |
| spellingShingle | David McIntyre Diana Arguijo Kaede Kawata Douglas Densmore Component library creation and pixel array generation with micromilled droplet microfluidics Microsystems & Nanoengineering |
| title | Component library creation and pixel array generation with micromilled droplet microfluidics |
| title_full | Component library creation and pixel array generation with micromilled droplet microfluidics |
| title_fullStr | Component library creation and pixel array generation with micromilled droplet microfluidics |
| title_full_unstemmed | Component library creation and pixel array generation with micromilled droplet microfluidics |
| title_short | Component library creation and pixel array generation with micromilled droplet microfluidics |
| title_sort | component library creation and pixel array generation with micromilled droplet microfluidics |
| url | https://doi.org/10.1038/s41378-024-00839-6 |
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