Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice
Previous studies described that allergic diseases, including asthma, occur less often than expected in patients with type 1 diabetes. Here, we investigated the influence of diabetes on allergic airway inflammation in a model of experimental asthma in mice. Diabetes was induced by intravenous injecti...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/6150843 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552557340262400 |
|---|---|
| author | Vinicius F. Carvalho Emiliano O. Barreto Ana Carolina S. Arantes Magda F. Serra Tatiana Paula T. Ferreira Yago A. P. Jannini-Sá Cory M. Hogaboam Marco A. Martins Patrícia M. R. Silva |
| author_facet | Vinicius F. Carvalho Emiliano O. Barreto Ana Carolina S. Arantes Magda F. Serra Tatiana Paula T. Ferreira Yago A. P. Jannini-Sá Cory M. Hogaboam Marco A. Martins Patrícia M. R. Silva |
| author_sort | Vinicius F. Carvalho |
| collection | DOAJ |
| description | Previous studies described that allergic diseases, including asthma, occur less often than expected in patients with type 1 diabetes. Here, we investigated the influence of diabetes on allergic airway inflammation in a model of experimental asthma in mice. Diabetes was induced by intravenous injection of alloxan into 12 h-fasted A/J mice, followed by subcutaneous sensitization with ovalbumin (OVA) and aluminum hydroxide (Al(OH)3), on days 5 and 19 after diabetes induction. Animals were intranasally challenged with OVA (25 μg), from day 24 to day 26. Alloxan-induced diabetes significantly attenuated airway inflammation as attested by the lower number of total leukocytes in the bronchoalveolar lavage fluid, mainly neutrophils and eosinophils. Suppression of eosinophil infiltration in the peribronchiolar space and generation of eosinophilotactic mediators, such as CCL-11/eotaxin, CCL-3/MIP-1α, and IL-5, were noted in the lungs of diabetic sensitized mice. In parallel, reduction of airway hyperreactivity (AHR) to methacholine, mucus production, and serum IgE levels was also noted under diabetic conditions. Our findings show that alloxan diabetes caused attenuation of lung allergic inflammatory response in A/J mice, by a mechanism possibly associated with downregulation of IgE antibody production. |
| format | Article |
| id | doaj-art-3729496824af4f908f834f475eee1eb8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-3729496824af4f908f834f475eee1eb82025-02-03T05:58:23ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/61508436150843Diabetes Downregulates Allergen-Induced Airway Inflammation in MiceVinicius F. Carvalho0Emiliano O. Barreto1Ana Carolina S. Arantes2Magda F. Serra3Tatiana Paula T. Ferreira4Yago A. P. Jannini-Sá5Cory M. Hogaboam6Marco A. Martins7Patrícia M. R. Silva8Laboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil No. 4365, 21045-900 Manguinhos, RJ, BrazilFederal University of Alagoas, 57072-970 Maceió, AL, BrazilLaboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil No. 4365, 21045-900 Manguinhos, RJ, BrazilLaboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil No. 4365, 21045-900 Manguinhos, RJ, BrazilLaboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil No. 4365, 21045-900 Manguinhos, RJ, BrazilLaboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil No. 4365, 21045-900 Manguinhos, RJ, BrazilCedars-Sinai Medical Center, 8700 Beverly Blvd, Los Angeles, CA 90048, USALaboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil No. 4365, 21045-900 Manguinhos, RJ, BrazilLaboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil No. 4365, 21045-900 Manguinhos, RJ, BrazilPrevious studies described that allergic diseases, including asthma, occur less often than expected in patients with type 1 diabetes. Here, we investigated the influence of diabetes on allergic airway inflammation in a model of experimental asthma in mice. Diabetes was induced by intravenous injection of alloxan into 12 h-fasted A/J mice, followed by subcutaneous sensitization with ovalbumin (OVA) and aluminum hydroxide (Al(OH)3), on days 5 and 19 after diabetes induction. Animals were intranasally challenged with OVA (25 μg), from day 24 to day 26. Alloxan-induced diabetes significantly attenuated airway inflammation as attested by the lower number of total leukocytes in the bronchoalveolar lavage fluid, mainly neutrophils and eosinophils. Suppression of eosinophil infiltration in the peribronchiolar space and generation of eosinophilotactic mediators, such as CCL-11/eotaxin, CCL-3/MIP-1α, and IL-5, were noted in the lungs of diabetic sensitized mice. In parallel, reduction of airway hyperreactivity (AHR) to methacholine, mucus production, and serum IgE levels was also noted under diabetic conditions. Our findings show that alloxan diabetes caused attenuation of lung allergic inflammatory response in A/J mice, by a mechanism possibly associated with downregulation of IgE antibody production.http://dx.doi.org/10.1155/2018/6150843 |
| spellingShingle | Vinicius F. Carvalho Emiliano O. Barreto Ana Carolina S. Arantes Magda F. Serra Tatiana Paula T. Ferreira Yago A. P. Jannini-Sá Cory M. Hogaboam Marco A. Martins Patrícia M. R. Silva Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice Mediators of Inflammation |
| title | Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice |
| title_full | Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice |
| title_fullStr | Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice |
| title_full_unstemmed | Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice |
| title_short | Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice |
| title_sort | diabetes downregulates allergen induced airway inflammation in mice |
| url | http://dx.doi.org/10.1155/2018/6150843 |
| work_keys_str_mv | AT viniciusfcarvalho diabetesdownregulatesallergeninducedairwayinflammationinmice AT emilianoobarreto diabetesdownregulatesallergeninducedairwayinflammationinmice AT anacarolinasarantes diabetesdownregulatesallergeninducedairwayinflammationinmice AT magdafserra diabetesdownregulatesallergeninducedairwayinflammationinmice AT tatianapaulatferreira diabetesdownregulatesallergeninducedairwayinflammationinmice AT yagoapjanninisa diabetesdownregulatesallergeninducedairwayinflammationinmice AT corymhogaboam diabetesdownregulatesallergeninducedairwayinflammationinmice AT marcoamartins diabetesdownregulatesallergeninducedairwayinflammationinmice AT patriciamrsilva diabetesdownregulatesallergeninducedairwayinflammationinmice |