An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions
Host-virus interactions are critically important for various stages of the viral replication cycle. The reliance of viruses on the host factors for their entry, replication, and maturation processes can be exploited for the development of antiviral therapeutics. Thus, the identification and characte...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2024-01-01
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| Series: | QRB Discovery |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S263328922400019X/type/journal_article |
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| _version_ | 1832542502797705216 |
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| author | Jiaqi Li Zhewang Lin |
| author_facet | Jiaqi Li Zhewang Lin |
| author_sort | Jiaqi Li |
| collection | DOAJ |
| description | Host-virus interactions are critically important for various stages of the viral replication cycle. The reliance of viruses on the host factors for their entry, replication, and maturation processes can be exploited for the development of antiviral therapeutics. Thus, the identification and characterization of such viral-host dependency factors has been an attractive area of research to provide novel antiviral targets. Traditional proteomic efforts based on affinity purification of protein complexes from cell lysates are limited to detecting strong and stable interactions. In this perspective, we discuss the integration of two latest proteomic techniques, based on in situ proximity labelling and chemical crosslinking methods, to uncover host-virus protein–protein interactions in living cells. |
| format | Article |
| id | doaj-art-3720660617584c829639d9f6ad2bbdb2 |
| institution | Kabale University |
| issn | 2633-2892 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | QRB Discovery |
| spelling | doaj-art-3720660617584c829639d9f6ad2bbdb22025-02-04T03:42:22ZengCambridge University PressQRB Discovery2633-28922024-01-01510.1017/qrd.2024.19An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactionsJiaqi Li0https://orcid.org/0009-0004-0502-3313Zhewang Lin1https://orcid.org/0000-0003-1301-6661Department of Biological Sciences, National University of Singapore, SingaporeDepartment of Biological Sciences, National University of Singapore, SingaporeHost-virus interactions are critically important for various stages of the viral replication cycle. The reliance of viruses on the host factors for their entry, replication, and maturation processes can be exploited for the development of antiviral therapeutics. Thus, the identification and characterization of such viral-host dependency factors has been an attractive area of research to provide novel antiviral targets. Traditional proteomic efforts based on affinity purification of protein complexes from cell lysates are limited to detecting strong and stable interactions. In this perspective, we discuss the integration of two latest proteomic techniques, based on in situ proximity labelling and chemical crosslinking methods, to uncover host-virus protein–protein interactions in living cells.https://www.cambridge.org/core/product/identifier/S263328922400019X/type/journal_articledynamicsvirologyprotein–protein interactions |
| spellingShingle | Jiaqi Li Zhewang Lin An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions QRB Discovery dynamics virology protein–protein interactions |
| title | An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions |
| title_full | An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions |
| title_fullStr | An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions |
| title_full_unstemmed | An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions |
| title_short | An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions |
| title_sort | integrated approach using proximity labelling and chemical crosslinking to probe in situ host virus protein protein interactions |
| topic | dynamics virology protein–protein interactions |
| url | https://www.cambridge.org/core/product/identifier/S263328922400019X/type/journal_article |
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