Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry
Early production of melon plant (Cucumis melo) is carried out using tunnels structures, where extreme temperatures lead to high reactive oxygen species production and, hence, oxidative stress. Malondialdehyde (MDA) is a recognized biomarker of the advanced oxidative status in a biological system. Th...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/4327954 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558024459288576 |
|---|---|
| author | Melisa E. Yonny Ariel Rodríguez Torressi Mónica A. Nazareno Soledad Cerutti |
| author_facet | Melisa E. Yonny Ariel Rodríguez Torressi Mónica A. Nazareno Soledad Cerutti |
| author_sort | Melisa E. Yonny |
| collection | DOAJ |
| description | Early production of melon plant (Cucumis melo) is carried out using tunnels structures, where extreme temperatures lead to high reactive oxygen species production and, hence, oxidative stress. Malondialdehyde (MDA) is a recognized biomarker of the advanced oxidative status in a biological system. Thus a reliable, sensitive, simple, selective, and rapid separative strategy based on ultra-high-performance liquid chromatography coupled to positive electrospray-tandem mass spectrometry (UPLC-(+)ESI-MS/MS) was developed for the first time to measure MDA, without derivatization, in leaves of melon plants exposed to stress conditions. The detection and quantitation limits were 0.02 μg·L−1 and 0.08 μg·L−1, respectively, which was demonstrated to be better than the methodologies currently reported in the literature. The accuracy values were between 96% and 104%. The precision intraday and interday values were 2.7% and 3.8%, respectively. The optimized methodology was applied to monitoring of changes in MDA levels between control and exposed to thermal stress conditions melon leaves samples. Important preliminary conclusions were obtained. Besides, a comparison between MDA levels in melon leaves quantified by the proposed method and the traditional thiobarbituric acid reactive species (TBARS) approach was undertaken. The MDA determination by TBARS could lead to unrealistic conclusions regarding the oxidative stress status in plants. |
| format | Article |
| id | doaj-art-36acc1d54ea74f398d7f90512ded6b99 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-36acc1d54ea74f398d7f90512ded6b992025-02-03T01:33:24ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/43279544327954Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass SpectrometryMelisa E. Yonny0Ariel Rodríguez Torressi1Mónica A. Nazareno2Soledad Cerutti3CITSE-CONICET, Universidad Nacional de Santiago del Estero, 4200 Santiago del Estero, ArgentinaINTA, 4200 Santiago del Estero, ArgentinaCITSE-CONICET, Universidad Nacional de Santiago del Estero, 4200 Santiago del Estero, ArgentinaInstituto de Química de San Luis (CONICET-UNSL), Área de Química Analítica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, 5700 San Luis, ArgentinaEarly production of melon plant (Cucumis melo) is carried out using tunnels structures, where extreme temperatures lead to high reactive oxygen species production and, hence, oxidative stress. Malondialdehyde (MDA) is a recognized biomarker of the advanced oxidative status in a biological system. Thus a reliable, sensitive, simple, selective, and rapid separative strategy based on ultra-high-performance liquid chromatography coupled to positive electrospray-tandem mass spectrometry (UPLC-(+)ESI-MS/MS) was developed for the first time to measure MDA, without derivatization, in leaves of melon plants exposed to stress conditions. The detection and quantitation limits were 0.02 μg·L−1 and 0.08 μg·L−1, respectively, which was demonstrated to be better than the methodologies currently reported in the literature. The accuracy values were between 96% and 104%. The precision intraday and interday values were 2.7% and 3.8%, respectively. The optimized methodology was applied to monitoring of changes in MDA levels between control and exposed to thermal stress conditions melon leaves samples. Important preliminary conclusions were obtained. Besides, a comparison between MDA levels in melon leaves quantified by the proposed method and the traditional thiobarbituric acid reactive species (TBARS) approach was undertaken. The MDA determination by TBARS could lead to unrealistic conclusions regarding the oxidative stress status in plants.http://dx.doi.org/10.1155/2017/4327954 |
| spellingShingle | Melisa E. Yonny Ariel Rodríguez Torressi Mónica A. Nazareno Soledad Cerutti Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | development of a novel sensitive selective and fast methodology to determine malondialdehyde in leaves of melon plants by ultra high performance liquid chromatography tandem mass spectrometry |
| url | http://dx.doi.org/10.1155/2017/4327954 |
| work_keys_str_mv | AT melisaeyonny developmentofanovelsensitiveselectiveandfastmethodologytodeterminemalondialdehydeinleavesofmelonplantsbyultrahighperformanceliquidchromatographytandemmassspectrometry AT arielrodrigueztorressi developmentofanovelsensitiveselectiveandfastmethodologytodeterminemalondialdehydeinleavesofmelonplantsbyultrahighperformanceliquidchromatographytandemmassspectrometry AT monicaanazareno developmentofanovelsensitiveselectiveandfastmethodologytodeterminemalondialdehydeinleavesofmelonplantsbyultrahighperformanceliquidchromatographytandemmassspectrometry AT soledadcerutti developmentofanovelsensitiveselectiveandfastmethodologytodeterminemalondialdehydeinleavesofmelonplantsbyultrahighperformanceliquidchromatographytandemmassspectrometry |