The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern Transylvania
This study examines the prevalence and the mechanisms of antibiotic resistance in <i>Pseudomonas aeruginosa</i> isolates collected from healthcare units in Northwestern Transylvania, Romania, between 2022 and 2023. Given the alarming rise in antibiotic resistance, the study screened 34 i...
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MDPI AG
2024-12-01
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| Series: | Biomolecules |
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| author | Matei-Ștefan Dobrescu Dan-Alexandru Țoc Adrian-Gabriel Pană Carmen Costache Anca Butiuc-Keul |
| author_facet | Matei-Ștefan Dobrescu Dan-Alexandru Țoc Adrian-Gabriel Pană Carmen Costache Anca Butiuc-Keul |
| author_sort | Matei-Ștefan Dobrescu |
| collection | DOAJ |
| description | This study examines the prevalence and the mechanisms of antibiotic resistance in <i>Pseudomonas aeruginosa</i> isolates collected from healthcare units in Northwestern Transylvania, Romania, between 2022 and 2023. Given the alarming rise in antibiotic resistance, the study screened 34 isolates for resistance to 10 antibiotics, 46 ARGs, and integrase genes using PCR analysis. The results reveal a concerning increase in multidrug-resistant (MDR) and extensively drug-resistant (XDR) isolates over the two-year period. Notably, the prevalence of ARGs encoding resistance to sulfonamides and beta-lactams, particularly <i>sul1</i> and <i>bla<sub>OXA-50</sub></i>, has shown a significant rise. Furthermore, the study detected the emergence of new resistance mechanisms in the same time interval. These include target protection and even more specific mechanisms, such as metallo-beta-lactamases or enzymes involved in the methylation of 23S rRNA. Statistical analysis further confirmed the correlation between Class I integrons and several ARGs, underscoring the role of horizontal gene transfer in the dissemination of resistance. These findings emphasize the urgent need for updated treatment strategies and monitoring programs to effectively combat the spread of ARGs in clinical settings. |
| format | Article |
| id | doaj-art-368a0aad1d804ae89d1e6b5e422e6101 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-368a0aad1d804ae89d1e6b5e422e61012025-01-24T13:24:48ZengMDPI AGBiomolecules2218-273X2024-12-01151110.3390/biom15010001The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern TransylvaniaMatei-Ștefan Dobrescu0Dan-Alexandru Țoc1Adrian-Gabriel Pană2Carmen Costache3Anca Butiuc-Keul4Doctoral School of Integrative Biology, Babeș-Bolyai University, 1 M. Kogalniceanu Street, 400084 Cluj-Napoca, RomaniaDepartment of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaFaculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeș-Bolyai University, 1 M. Kogalniceanu Street, 400084 Cluj-Napoca, RomaniaThis study examines the prevalence and the mechanisms of antibiotic resistance in <i>Pseudomonas aeruginosa</i> isolates collected from healthcare units in Northwestern Transylvania, Romania, between 2022 and 2023. Given the alarming rise in antibiotic resistance, the study screened 34 isolates for resistance to 10 antibiotics, 46 ARGs, and integrase genes using PCR analysis. The results reveal a concerning increase in multidrug-resistant (MDR) and extensively drug-resistant (XDR) isolates over the two-year period. Notably, the prevalence of ARGs encoding resistance to sulfonamides and beta-lactams, particularly <i>sul1</i> and <i>bla<sub>OXA-50</sub></i>, has shown a significant rise. Furthermore, the study detected the emergence of new resistance mechanisms in the same time interval. These include target protection and even more specific mechanisms, such as metallo-beta-lactamases or enzymes involved in the methylation of 23S rRNA. Statistical analysis further confirmed the correlation between Class I integrons and several ARGs, underscoring the role of horizontal gene transfer in the dissemination of resistance. These findings emphasize the urgent need for updated treatment strategies and monitoring programs to effectively combat the spread of ARGs in clinical settings.https://www.mdpi.com/2218-273X/15/1/1antibiotic resistance genesantibiotic resistance mechanismsintegronsmultidrug-resistant bacteria<i>Pseudomonas aeruginosa</i> |
| spellingShingle | Matei-Ștefan Dobrescu Dan-Alexandru Țoc Adrian-Gabriel Pană Carmen Costache Anca Butiuc-Keul The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern Transylvania Biomolecules antibiotic resistance genes antibiotic resistance mechanisms integrons multidrug-resistant bacteria <i>Pseudomonas aeruginosa</i> |
| title | The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern Transylvania |
| title_full | The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern Transylvania |
| title_fullStr | The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern Transylvania |
| title_full_unstemmed | The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern Transylvania |
| title_short | The Difference a Year Can Make: How Antibiotic Resistance Mechanisms in <i>Pseudomonas aeruginosa</i> Have Changed in Northwestern Transylvania |
| title_sort | difference a year can make how antibiotic resistance mechanisms in i pseudomonas aeruginosa i have changed in northwestern transylvania |
| topic | antibiotic resistance genes antibiotic resistance mechanisms integrons multidrug-resistant bacteria <i>Pseudomonas aeruginosa</i> |
| url | https://www.mdpi.com/2218-273X/15/1/1 |
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