Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification
The endocannabinoids N-arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups of N-acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannab...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/2426398 |
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| author | Jantana Keereetaweep Kent D. Chapman |
| author_facet | Jantana Keereetaweep Kent D. Chapman |
| author_sort | Jantana Keereetaweep |
| collection | DOAJ |
| description | The endocannabinoids N-arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups of N-acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannabinoid receptors found in various organisms. After AEA and 2-AG were discovered in the 1990s, they have been extensively documented to have a broad range of physiological functions. Along with AEA, several NAEs, for example, N-palmitoylethanolamine (PEA), N-stearoylethanolamine (SEA), and N-oleoylethanolamine (OEA) are also present in tissues, usually at much larger concentrations than AEA. Any perturbation that involves the endocannabinoid pathway may subsequently alter basal level or metabolism of these lipid mediators. Further, the altered levels of these molecules often reflect pathological conditions associated with tissue damage. Robust and sensitive methodologies to analyze these lipid mediators are essential to understanding how they act as endocannabinoids. The recent advances in mass spectrometry allow researchers to develop lipidomics approaches and several methodologies have been proposed to quantify endocannabinoids in various biological systems. |
| format | Article |
| id | doaj-art-3688e47be37040b791d13da13f06e4c0 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Neural Plasticity |
| spelling | doaj-art-3688e47be37040b791d13da13f06e4c02025-02-03T05:46:46ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/24263982426398Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and QuantificationJantana Keereetaweep0Kent D. Chapman1Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203, USADepartment of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203, USAThe endocannabinoids N-arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups of N-acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannabinoid receptors found in various organisms. After AEA and 2-AG were discovered in the 1990s, they have been extensively documented to have a broad range of physiological functions. Along with AEA, several NAEs, for example, N-palmitoylethanolamine (PEA), N-stearoylethanolamine (SEA), and N-oleoylethanolamine (OEA) are also present in tissues, usually at much larger concentrations than AEA. Any perturbation that involves the endocannabinoid pathway may subsequently alter basal level or metabolism of these lipid mediators. Further, the altered levels of these molecules often reflect pathological conditions associated with tissue damage. Robust and sensitive methodologies to analyze these lipid mediators are essential to understanding how they act as endocannabinoids. The recent advances in mass spectrometry allow researchers to develop lipidomics approaches and several methodologies have been proposed to quantify endocannabinoids in various biological systems.http://dx.doi.org/10.1155/2016/2426398 |
| spellingShingle | Jantana Keereetaweep Kent D. Chapman Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification Neural Plasticity |
| title | Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification |
| title_full | Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification |
| title_fullStr | Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification |
| title_full_unstemmed | Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification |
| title_short | Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification |
| title_sort | lipidomic analysis of endocannabinoid signaling targeted metabolite identification and quantification |
| url | http://dx.doi.org/10.1155/2016/2426398 |
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