New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations
New simple and sensitive spectrophotometric and fluorimetric methods have been developed and validated for the determination of fluoxetine hydrochloride (FLX) in its pharmaceutical formulations. The spectrophotometric method was based on the reaction of FLX with 1,2-naphthoquinone-4-sulphonate (NQS)...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2009/257306 |
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| author | Ibrahim A. Darwish Sawsan M. Amer Heba H. Abdine Lama I. Al-Rayes |
| author_facet | Ibrahim A. Darwish Sawsan M. Amer Heba H. Abdine Lama I. Al-Rayes |
| author_sort | Ibrahim A. Darwish |
| collection | DOAJ |
| description | New simple and sensitive spectrophotometric and fluorimetric
methods have been developed and validated for the determination of
fluoxetine hydrochloride (FLX) in its pharmaceutical formulations.
The spectrophotometric method was based on the reaction of FLX
with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium
(pH 11) to form an orange-colored product that was measured at 490
nm. The fluorimetric method was based on the reaction of FLX with
4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in an alkaline
medium (pH 8) to form a highly fluorescent product that was
measured at 545 nm after excitation at 490 nm. The variables
affecting the reactions of FLX with both NQS and NBD-Cl were
carefully studied and optimized. The kinetics of the reactions
were investigated, and the reaction mechanisms were presented.
Under the optimum reaction conditions, good linear relationships
were found between the readings and the concentrations of FLX in
the ranges of 0.3–6 and 0.035–0.5 μg mL−1 for the
spectrophotometric and fluorimetric methods, respectively. The
limits of detection were 0.1 and 0.01 μg mL−1 for the
spectrophotometric and fluorimetric methods, respectively. Both
methods were successfully applied to the determination of FLX in
its pharmaceutical formulations. |
| format | Article |
| id | doaj-art-367ff89e310b4a8691b4adea3e8d8680 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-367ff89e310b4a8691b4adea3e8d86802025-02-03T06:13:47ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792009-01-01200910.1155/2009/257306257306New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical FormulationsIbrahim A. Darwish0Sawsan M. Amer1Heba H. Abdine2Lama I. Al-Rayes3Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaNew simple and sensitive spectrophotometric and fluorimetric methods have been developed and validated for the determination of fluoxetine hydrochloride (FLX) in its pharmaceutical formulations. The spectrophotometric method was based on the reaction of FLX with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium (pH 11) to form an orange-colored product that was measured at 490 nm. The fluorimetric method was based on the reaction of FLX with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in an alkaline medium (pH 8) to form a highly fluorescent product that was measured at 545 nm after excitation at 490 nm. The variables affecting the reactions of FLX with both NQS and NBD-Cl were carefully studied and optimized. The kinetics of the reactions were investigated, and the reaction mechanisms were presented. Under the optimum reaction conditions, good linear relationships were found between the readings and the concentrations of FLX in the ranges of 0.3–6 and 0.035–0.5 μg mL−1 for the spectrophotometric and fluorimetric methods, respectively. The limits of detection were 0.1 and 0.01 μg mL−1 for the spectrophotometric and fluorimetric methods, respectively. Both methods were successfully applied to the determination of FLX in its pharmaceutical formulations.http://dx.doi.org/10.1155/2009/257306 |
| spellingShingle | Ibrahim A. Darwish Sawsan M. Amer Heba H. Abdine Lama I. Al-Rayes New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations International Journal of Analytical Chemistry |
| title | New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations |
| title_full | New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations |
| title_fullStr | New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations |
| title_full_unstemmed | New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations |
| title_short | New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations |
| title_sort | new spectrophotometric and fluorimetric methods for determination of fluoxetine in pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2009/257306 |
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