INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS
The raw material for blood protein hydrolysate preparation is whole animal blood, its clots and other serum production wastes. The dependence of ВНК-21/2-17 cell lineage productivity on the season of hydrolysate raw material preparation was observed. Cell growth stimulated by blood protein hydrolys...
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Da Vinci Media
2018-05-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/349 |
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| author | M. A. Shevchenko M. N. Guseva D. A. Lozovoy M. I. Doronin D. V. Mikhalishin V. V. Mikhalishin |
| author_facet | M. A. Shevchenko M. N. Guseva D. A. Lozovoy M. I. Doronin D. V. Mikhalishin V. V. Mikhalishin |
| author_sort | M. A. Shevchenko |
| collection | DOAJ |
| description | The raw material for blood protein hydrolysate preparation is whole animal blood, its clots and other serum production wastes. The dependence of ВНК-21/2-17 cell lineage productivity on the season of hydrolysate raw material preparation was observed. Cell growth stimulated by blood protein hydrolysate from raw material prepared in summer months 1.2–1.3 times exceeded cell growth stimulated by blood protein hydrolysate prepared in autumn-winter period (differences are significant, p < 0,001). The amount of FMDV immunogenic components from “summer” blood protein hydrolysate 1.3–1.6 exceeded the amount obtained during other seasons of the year (differences are significant, p < 0,001). There was found a direct correlation between BHK-21/2-17 cell lineage productivity, concentration of FMDV immunogenic components and seasonal dynamics of the contents of the following amino acids composing blood protein hydrolysates: alanin, asparagine, valine, histidine, glycine, isoleucine, leucine, lysine, methionine, proline, serine, tyrosine, threonine, tryptophane, and phenylalanine. To increase BHK-21/2-17 cell lineage productivity and concentration of FMDV immunogenic components it was suggested to add some additional amino acids into the culture growth medium for suspension cell culture in autumn-summer period together with blood protein hydrolysate |
| format | Article |
| id | doaj-art-364d26bfa05443e6bf94e0ff8601c44f |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-364d26bfa05443e6bf94e0ff8601c44f2025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-05-0101555910.29326/2304-196X-2018-1-55-59349INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTSM. A. Shevchenko0M. N. Guseva1D. A. Lozovoy2M. I. Doronin3D. V. Mikhalishin4V. V. Mikhalishin5FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe raw material for blood protein hydrolysate preparation is whole animal blood, its clots and other serum production wastes. The dependence of ВНК-21/2-17 cell lineage productivity on the season of hydrolysate raw material preparation was observed. Cell growth stimulated by blood protein hydrolysate from raw material prepared in summer months 1.2–1.3 times exceeded cell growth stimulated by blood protein hydrolysate prepared in autumn-winter period (differences are significant, p < 0,001). The amount of FMDV immunogenic components from “summer” blood protein hydrolysate 1.3–1.6 exceeded the amount obtained during other seasons of the year (differences are significant, p < 0,001). There was found a direct correlation between BHK-21/2-17 cell lineage productivity, concentration of FMDV immunogenic components and seasonal dynamics of the contents of the following amino acids composing blood protein hydrolysates: alanin, asparagine, valine, histidine, glycine, isoleucine, leucine, lysine, methionine, proline, serine, tyrosine, threonine, tryptophane, and phenylalanine. To increase BHK-21/2-17 cell lineage productivity and concentration of FMDV immunogenic components it was suggested to add some additional amino acids into the culture growth medium for suspension cell culture in autumn-summer period together with blood protein hydrolysatehttps://veterinary.arriah.ru/jour/article/view/349blood protein hydrolysateamino acidsmonths of the yearproductivityfmdv immunogenic components |
| spellingShingle | M. A. Shevchenko M. N. Guseva D. A. Lozovoy M. I. Doronin D. V. Mikhalishin V. V. Mikhalishin INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS Ветеринария сегодня blood protein hydrolysate amino acids months of the year productivity fmdv immunogenic components |
| title | INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS |
| title_full | INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS |
| title_fullStr | INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS |
| title_full_unstemmed | INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS |
| title_short | INFLUENCE OF CHANGESIN AMINO ACID COMPOSITION OF BLOOD PROTEIN HYDROLYSATE ON THE PRODUCTIVITY OF ВНК-21/2-17 CELL LINEAGE AND AMOUNT OF FMDV IMMUNOGENIC COMPONENTS |
| title_sort | influence of changesin amino acid composition of blood protein hydrolysate on the productivity of внк 21 2 17 cell lineage and amount of fmdv immunogenic components |
| topic | blood protein hydrolysate amino acids months of the year productivity fmdv immunogenic components |
| url | https://veterinary.arriah.ru/jour/article/view/349 |
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