The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus
Objective: Diabetic cardiomyopathy (DCM) is characterized by complex pathophysiological events. miRNAs play a role in the DCM. In our study, the potential of miRNAs as a biomarker in the diagnosis and treatment of DCM was evaluated in the diabetes model induced by streptozotocin in rats. Methods: T...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2025-01-01
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| Series: | Bezmiâlem Science |
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| Online Access: | https://www.bezmialemscience.org/articles/the-effects-of-micrornas-on-cardiomyopathy-in-a-rat-model-of-streptozotocin-induced-diabetes-mellitus/doi/bas.galenos.2024.74429 |
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| author | Aslıhan Şeyda DOĞAN Mukaddes PALA Şenay GÖRÜCÜ YILMAZ Ayfer BECEREN Aydın KARABULUT Yalçın POLAT Hatice Kübra ELÇİOĞLU |
| author_facet | Aslıhan Şeyda DOĞAN Mukaddes PALA Şenay GÖRÜCÜ YILMAZ Ayfer BECEREN Aydın KARABULUT Yalçın POLAT Hatice Kübra ELÇİOĞLU |
| author_sort | Aslıhan Şeyda DOĞAN |
| collection | DOAJ |
| description | Objective: Diabetic cardiomyopathy (DCM) is characterized by complex pathophysiological events. miRNAs play a role in the DCM. In our study, the potential of miRNAs as a biomarker in the diagnosis and treatment of DCM was evaluated in the diabetes model induced by streptozotocin in rats.
Methods: Therefore, miRNAs obtained from rat heart tissue were analyzed by microarray, and twelve miRNAs (rno-miR-200c-3p, rno-miR-129-5p, rno-miR-150-3p, rno-miR-3584-5p, rno-miR-34c-3p, rno-miR-342-3p, rno-miR-466b-3p, rno-miR-466c-3p, rno-miR-31a-3p, rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) with changed expression levels were validated by real-time polymerase chain reaction analysis.
Results: As a result of the validation, it was determined that three miRNAs (rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) were downregulated, one miRNA (rno-miR-200c-3p) was upregulated (p<0.05). MiR-15b-5p, miR-196b-3p, miR-200c-3p, and miR-15b-5p are involved in the regulation of DCM, and the GRAP2 gene is one of the possible targets of these miRNAs.
Conclusion: miR-200c-3p has diagnostic value and may be a biomarker candidate. |
| format | Article |
| id | doaj-art-36419dc8c2dd459a97ef8b1b8f329303 |
| institution | Kabale University |
| issn | 2148-2373 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Bezmiâlem Science |
| spelling | doaj-art-36419dc8c2dd459a97ef8b1b8f3293032025-01-27T12:29:51ZengGalenos Publishing HouseBezmiâlem Science2148-23732025-01-01131354410.14235/bas.galenos.2024.74429The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes MellitusAslıhan Şeyda DOĞAN0https://orcid.org/0000-0001-9124-0648Mukaddes PALA1https://orcid.org/0000-0002-0610-0526Şenay GÖRÜCÜ YILMAZ2https://orcid.org/0000-0003-0523-7819Ayfer BECEREN3https://orcid.org/0000-0002-6770-6089Aydın KARABULUT4https://orcid.org/0000-0003-3969-8613Yalçın POLAT5https://orcid.org/0000-0003-0848-0814Hatice Kübra ELÇİOĞLU6https://orcid.org/0000-0002-3312-3029Biruni University Faculty of Medicine, Department of Medical Pharmacology, Istanbul, TürkiyeMalatya Turgut Özal University Faculty of Medicine, Department of Physiology, Malatya, TürkiyeGaziantep University Faculty of Health Sciences, Department of Nutrition and Dietetics, Gaziantep, TürkiyeMarmara University Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Istanbul, TürkiyeUniversity of Health Sciences Türkiye, Hamidiye Health Science Institute, Department of Immunology, Istanbul, TürkiyeBiruni University Faculty of Medicine, Department of Pathology, Istanbul, TürkiyeMarmara University Faculty of Pharmacy, Department of Pharmacology, Istanbul, TürkiyeObjective: Diabetic cardiomyopathy (DCM) is characterized by complex pathophysiological events. miRNAs play a role in the DCM. In our study, the potential of miRNAs as a biomarker in the diagnosis and treatment of DCM was evaluated in the diabetes model induced by streptozotocin in rats. Methods: Therefore, miRNAs obtained from rat heart tissue were analyzed by microarray, and twelve miRNAs (rno-miR-200c-3p, rno-miR-129-5p, rno-miR-150-3p, rno-miR-3584-5p, rno-miR-34c-3p, rno-miR-342-3p, rno-miR-466b-3p, rno-miR-466c-3p, rno-miR-31a-3p, rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) with changed expression levels were validated by real-time polymerase chain reaction analysis. Results: As a result of the validation, it was determined that three miRNAs (rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) were downregulated, one miRNA (rno-miR-200c-3p) was upregulated (p<0.05). MiR-15b-5p, miR-196b-3p, miR-200c-3p, and miR-15b-5p are involved in the regulation of DCM, and the GRAP2 gene is one of the possible targets of these miRNAs. Conclusion: miR-200c-3p has diagnostic value and may be a biomarker candidate.https://www.bezmialemscience.org/articles/the-effects-of-micrornas-on-cardiomyopathy-in-a-rat-model-of-streptozotocin-induced-diabetes-mellitus/doi/bas.galenos.2024.74429diabetic cardiomyopathymirna expressionbiomarkerstreptozotocin |
| spellingShingle | Aslıhan Şeyda DOĞAN Mukaddes PALA Şenay GÖRÜCÜ YILMAZ Ayfer BECEREN Aydın KARABULUT Yalçın POLAT Hatice Kübra ELÇİOĞLU The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus Bezmiâlem Science diabetic cardiomyopathy mirna expression biomarker streptozotocin |
| title | The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus |
| title_full | The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus |
| title_fullStr | The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus |
| title_full_unstemmed | The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus |
| title_short | The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus |
| title_sort | effects of micrornas on cardiomyopathy in a rat model of streptozotocin induced diabetes mellitus |
| topic | diabetic cardiomyopathy mirna expression biomarker streptozotocin |
| url | https://www.bezmialemscience.org/articles/the-effects-of-micrornas-on-cardiomyopathy-in-a-rat-model-of-streptozotocin-induced-diabetes-mellitus/doi/bas.galenos.2024.74429 |
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