Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances
The measure of O2 consumption during the inhibited autoxidation of an easily oxidizable substrate is one of the most reliable and predictive methods to assess antioxidant activity, especially for structure-activity relationship studies, for food and industrial applications. The differential oxygen u...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/6369358 |
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| author | Riccardo Amorati Andrea Baschieri Luca Valgimigli |
| author_facet | Riccardo Amorati Andrea Baschieri Luca Valgimigli |
| author_sort | Riccardo Amorati |
| collection | DOAJ |
| description | The measure of O2 consumption during the inhibited autoxidation of an easily oxidizable substrate is one of the most reliable and predictive methods to assess antioxidant activity, especially for structure-activity relationship studies, for food and industrial applications. The differential oxygen uptake apparatus described herein represents a powerful and cost-effective way to obtain antioxidant activity from inhibited autoxidation studies. These experiments provide the rate constant and the stoichiometry of the reaction between antioxidants and peroxyl radicals (ROO∙), which are involved in the propagation of radical damage. We show the operation principles and the utility of this instrumentation in the bioorganic laboratory, with regard to the recent advances in this field, ranging from the study of natural antioxidants in biomimetic system, to the use of substrates generating hydroperoxyl radicals, and to the evaluation of novel nanoantioxidants. |
| format | Article |
| id | doaj-art-363f0768991247a5b9aa901f934a77e5 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-363f0768991247a5b9aa901f934a77e52025-02-03T01:33:06ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/63693586369358Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent AdvancesRiccardo Amorati0Andrea Baschieri1Luca Valgimigli2Department of Chemistry “G. Ciamician”, University of Bologna, Via S. Giacomo 11, 40126 Bologna, ItalyDepartment of Chemistry “G. Ciamician”, University of Bologna, Via S. Giacomo 11, 40126 Bologna, ItalyDepartment of Chemistry “G. Ciamician”, University of Bologna, Via S. Giacomo 11, 40126 Bologna, ItalyThe measure of O2 consumption during the inhibited autoxidation of an easily oxidizable substrate is one of the most reliable and predictive methods to assess antioxidant activity, especially for structure-activity relationship studies, for food and industrial applications. The differential oxygen uptake apparatus described herein represents a powerful and cost-effective way to obtain antioxidant activity from inhibited autoxidation studies. These experiments provide the rate constant and the stoichiometry of the reaction between antioxidants and peroxyl radicals (ROO∙), which are involved in the propagation of radical damage. We show the operation principles and the utility of this instrumentation in the bioorganic laboratory, with regard to the recent advances in this field, ranging from the study of natural antioxidants in biomimetic system, to the use of substrates generating hydroperoxyl radicals, and to the evaluation of novel nanoantioxidants.http://dx.doi.org/10.1155/2017/6369358 |
| spellingShingle | Riccardo Amorati Andrea Baschieri Luca Valgimigli Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances Journal of Chemistry |
| title | Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances |
| title_full | Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances |
| title_fullStr | Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances |
| title_full_unstemmed | Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances |
| title_short | Measuring Antioxidant Activity in Bioorganic Samples by the Differential Oxygen Uptake Apparatus: Recent Advances |
| title_sort | measuring antioxidant activity in bioorganic samples by the differential oxygen uptake apparatus recent advances |
| url | http://dx.doi.org/10.1155/2017/6369358 |
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