Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy
Aim. To evaluate the effect of vascular endothelial growth factor A (VEGF-A) on the subpopulation composition of monocytes in the blood mononuclear cell culture of patients with coronary heart disease (CHD), with and without ischemic cardiomyopathy (ICMP).Materials and methods. A single-center, expe...
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Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
2025-05-01
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| Series: | Сеченовский вестник |
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| Online Access: | https://www.sechenovmedj.com/jour/article/view/1255 |
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| author | M. V. Gladkovskaya S. P. Chumakova O. I. Urazova V. S. Poletika V. M. Shipulin S. L. Andreev |
| author_facet | M. V. Gladkovskaya S. P. Chumakova O. I. Urazova V. S. Poletika V. M. Shipulin S. L. Andreev |
| author_sort | M. V. Gladkovskaya |
| collection | DOAJ |
| description | Aim. To evaluate the effect of vascular endothelial growth factor A (VEGF-A) on the subpopulation composition of monocytes in the blood mononuclear cell culture of patients with coronary heart disease (CHD), with and without ischemic cardiomyopathy (ICMP).Materials and methods. A single-center, experimental in vitro study was conducted. The study included 22 patients with CHD: 11 with ICMP, 11 without ICMP, and 10 healthy donors. Blood mononuclei were isolated from venous blood by immunomagnetic separation for CD14 and CD34 antigens, then incubated with and without the addition of VEGF-A 50 ng/mL (control and stimulated samples). After 6 days, the total monocyte content, the proportion of classical CD14++CD16–, intermediate CD14++CD16+, non-classical CD14+CD16++, and transitional CD14+CD16– monocytes were assessed using flow cytofluorimetry.Results. In groups of patients with CHD and in those groups where the patients were considered relatively healthy, a decrease in the content of CD14++CD16+ in the control and stimulated samples was shown. Only in the CHD group with ICMP relative to the control sample, after VEGF-A stimulation, a statistically significant increase in all CD14+ was found: 10.63% (6.80; 17.64) vs. 15.28% (8.75; 27.99), p < 0.01, and their subpopulations: CD14++CD16−: 6.08% (1.76; 8.84) vs. 8.57% (3.51; 16.8), p < 0.05, CD14++CD16+: 3.64% (2.03; 8.59) vs. 6.26% (3.87; 10.3), p < 0.05. In the same group, a tendency towards an increase in CD14+CD16++ was noted after stimulation: 0.19% (0.18; 1.11) vs. 0.61% (0.37; 1.58), p = 0.062. No differences in the content of all monocytes and their subpopulations after VEGF-A stimulation were found in the CHD without ICMP group nor in the healthy group. The content of CD14+CD16– in all groups in the control and stimulated samples did not differ.Conclusion. CHD is characterized by a deficiency of all CD14+ cells and intermediate monocytes due to their transdifferentiation. VEGF-A affects the subpopulation composition of monocytes in CHD only in the presence of ICMP by increasing the content of all CD14+ cells, and in their intermediate and classical forms without exceeding the indicators in healthy donors. |
| format | Article |
| id | doaj-art-362f68b55cf04e22a1da81cff959e44a |
| institution | Kabale University |
| issn | 2218-7332 2658-3348 |
| language | Russian |
| publishDate | 2025-05-01 |
| publisher | Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University) |
| record_format | Article |
| series | Сеченовский вестник |
| spelling | doaj-art-362f68b55cf04e22a1da81cff959e44a2025-08-20T03:57:51ZrusFederal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)Сеченовский вестник2218-73322658-33482025-05-01161203310.47093/2218-7332.2025.16.1.20-33615Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathyM. V. Gladkovskaya0S. P. Chumakova1O. I. Urazova2V. S. Poletika3V. M. Shipulin4S. L. Andreev5Siberian State Medical UniversitySiberian State Medical University; Immanuel Kant Baltic Federal UniversitySiberian State Medical University; Tomsk State University of Control Systems and RadioelectronicsSiberian State Medical UniversityTomsk National Research Medical Center of the Russian Academy of SciencesTomsk National Research Medical Center of the Russian Academy of SciencesAim. To evaluate the effect of vascular endothelial growth factor A (VEGF-A) on the subpopulation composition of monocytes in the blood mononuclear cell culture of patients with coronary heart disease (CHD), with and without ischemic cardiomyopathy (ICMP).Materials and methods. A single-center, experimental in vitro study was conducted. The study included 22 patients with CHD: 11 with ICMP, 11 without ICMP, and 10 healthy donors. Blood mononuclei were isolated from venous blood by immunomagnetic separation for CD14 and CD34 antigens, then incubated with and without the addition of VEGF-A 50 ng/mL (control and stimulated samples). After 6 days, the total monocyte content, the proportion of classical CD14++CD16–, intermediate CD14++CD16+, non-classical CD14+CD16++, and transitional CD14+CD16– monocytes were assessed using flow cytofluorimetry.Results. In groups of patients with CHD and in those groups where the patients were considered relatively healthy, a decrease in the content of CD14++CD16+ in the control and stimulated samples was shown. Only in the CHD group with ICMP relative to the control sample, after VEGF-A stimulation, a statistically significant increase in all CD14+ was found: 10.63% (6.80; 17.64) vs. 15.28% (8.75; 27.99), p < 0.01, and their subpopulations: CD14++CD16−: 6.08% (1.76; 8.84) vs. 8.57% (3.51; 16.8), p < 0.05, CD14++CD16+: 3.64% (2.03; 8.59) vs. 6.26% (3.87; 10.3), p < 0.05. In the same group, a tendency towards an increase in CD14+CD16++ was noted after stimulation: 0.19% (0.18; 1.11) vs. 0.61% (0.37; 1.58), p = 0.062. No differences in the content of all monocytes and their subpopulations after VEGF-A stimulation were found in the CHD without ICMP group nor in the healthy group. The content of CD14+CD16– in all groups in the control and stimulated samples did not differ.Conclusion. CHD is characterized by a deficiency of all CD14+ cells and intermediate monocytes due to their transdifferentiation. VEGF-A affects the subpopulation composition of monocytes in CHD only in the presence of ICMP by increasing the content of all CD14+ cells, and in their intermediate and classical forms without exceeding the indicators in healthy donors.https://www.sechenovmedj.com/jour/article/view/1255vegf-adifferentiationangiogenesisclassic monocytescd14++cd16–intermediate monocytescd14++cd16+non-classic monocytescd14+cd16++transient monocytescd14+cd16– |
| spellingShingle | M. V. Gladkovskaya S. P. Chumakova O. I. Urazova V. S. Poletika V. M. Shipulin S. L. Andreev Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy Сеченовский вестник vegf-a differentiation angiogenesis classic monocytes cd14++cd16– intermediate monocytes cd14++cd16+ non-classic monocytes cd14+cd16++ transient monocytes cd14+cd16– |
| title | Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy |
| title_full | Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy |
| title_fullStr | Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy |
| title_full_unstemmed | Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy |
| title_short | Vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy |
| title_sort | vascular endothelial growth factor attenuates enhanced spontaneous transdifferentiation of classical and intermediate monocytes in patients with ischemic cardiomyopathy |
| topic | vegf-a differentiation angiogenesis classic monocytes cd14++cd16– intermediate monocytes cd14++cd16+ non-classic monocytes cd14+cd16++ transient monocytes cd14+cd16– |
| url | https://www.sechenovmedj.com/jour/article/view/1255 |
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