Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study.
There are currently no reliable, established serum biomarkers to predict the prognosis of radiotherapy for advanced cervical cancer. We aimed to identify serum biomarkers for survival after radiotherapy for cervical cancer. In this multicenter prospective cohort study, the usefulness of pre- and pos...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0259235&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832595670907748352 |
|---|---|
| author | Yoko Harima Takuro Ariga Yuko Kaneyasu Hitoshi Ikushima Sunao Tokumaru Shigetoshi Shimamoto Takeo Takahashi Noriko Ii Kayoko Tsujino Anneyuko I Saito Hiroki Ushijima Takafumi Toita Tatsuya Ohno |
| author_facet | Yoko Harima Takuro Ariga Yuko Kaneyasu Hitoshi Ikushima Sunao Tokumaru Shigetoshi Shimamoto Takeo Takahashi Noriko Ii Kayoko Tsujino Anneyuko I Saito Hiroki Ushijima Takafumi Toita Tatsuya Ohno |
| author_sort | Yoko Harima |
| collection | DOAJ |
| description | There are currently no reliable, established serum biomarkers to predict the prognosis of radiotherapy for advanced cervical cancer. We aimed to identify serum biomarkers for survival after radiotherapy for cervical cancer. In this multicenter prospective cohort study, the usefulness of pre- and posttreatment serum protein levels of potential biomarkers, including squamous cell carcinoma antigen (SCC-Ag), apolipoprotein C-II (ApoC-II), matrix metalloproteinase (MMP)1, and MMP2, were evaluated together with clinical factors in 145 cervical cancer patients in order to determine their suitability to predict survival. Progression-free survival (PFS) was the primary endpoint, and overall survival (OS), pelvic PFS (PPFS), and distant metastasis-free survival (DMFS) were the secondary endpoints. Blood samples were collected before and 1 month after radiotherapy to measure serum biomarker levels. ApoC-II was measured using a monoclonal antibody-based enzyme-linked immunosorbent assay, which was developed for this purpose. Kaplan-Meier method, log-rank test, and univariate and multivariate Cox proportional hazards models were used for statistical analyses. In multivariate analysis, larger tumor size was independently associated with shorter PFS, OS, PPFS, and DMFS, while longer overall treatment time was independently associated with shorter PPFS. Higher pretreatment SCC-Ag (P < 0.001) was associated with shorter DMFS. Higher posttreatment SCC-Ag (P = 0.017) was also associated with shorter DMFS. Pretreatment ApoC-II was associated with PPFS in univariate analysis (P = 0.048), but not in multivariate analysis. Patients with pretreatment ApoC-II levels ≤ 25.8 μg/ml had shorter PPFS than those with pretreatment ApoC-II levels > 25.8 μg/ml (P = 0.023, log-rank test). Pre- and posttreatment serum SCC-Ag and pretreatment serum ApoC-II levels may be important biomarkers to predict survival outcomes of patients with cervical cancer after radiotherapy. Pre- and posttreatment SCC-Ag and pretreatment ApoC-II might be useful in clinical settings for screening patients to improve treatment strategies in cervical cancer. |
| format | Article |
| id | doaj-art-35fe0528408448aa868e60daef9e04e8 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-35fe0528408448aa868e60daef9e04e82025-01-18T05:31:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011611e025923510.1371/journal.pone.0259235Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study.Yoko HarimaTakuro ArigaYuko KaneyasuHitoshi IkushimaSunao TokumaruShigetoshi ShimamotoTakeo TakahashiNoriko IiKayoko TsujinoAnneyuko I SaitoHiroki UshijimaTakafumi ToitaTatsuya OhnoThere are currently no reliable, established serum biomarkers to predict the prognosis of radiotherapy for advanced cervical cancer. We aimed to identify serum biomarkers for survival after radiotherapy for cervical cancer. In this multicenter prospective cohort study, the usefulness of pre- and posttreatment serum protein levels of potential biomarkers, including squamous cell carcinoma antigen (SCC-Ag), apolipoprotein C-II (ApoC-II), matrix metalloproteinase (MMP)1, and MMP2, were evaluated together with clinical factors in 145 cervical cancer patients in order to determine their suitability to predict survival. Progression-free survival (PFS) was the primary endpoint, and overall survival (OS), pelvic PFS (PPFS), and distant metastasis-free survival (DMFS) were the secondary endpoints. Blood samples were collected before and 1 month after radiotherapy to measure serum biomarker levels. ApoC-II was measured using a monoclonal antibody-based enzyme-linked immunosorbent assay, which was developed for this purpose. Kaplan-Meier method, log-rank test, and univariate and multivariate Cox proportional hazards models were used for statistical analyses. In multivariate analysis, larger tumor size was independently associated with shorter PFS, OS, PPFS, and DMFS, while longer overall treatment time was independently associated with shorter PPFS. Higher pretreatment SCC-Ag (P < 0.001) was associated with shorter DMFS. Higher posttreatment SCC-Ag (P = 0.017) was also associated with shorter DMFS. Pretreatment ApoC-II was associated with PPFS in univariate analysis (P = 0.048), but not in multivariate analysis. Patients with pretreatment ApoC-II levels ≤ 25.8 μg/ml had shorter PPFS than those with pretreatment ApoC-II levels > 25.8 μg/ml (P = 0.023, log-rank test). Pre- and posttreatment serum SCC-Ag and pretreatment serum ApoC-II levels may be important biomarkers to predict survival outcomes of patients with cervical cancer after radiotherapy. Pre- and posttreatment SCC-Ag and pretreatment ApoC-II might be useful in clinical settings for screening patients to improve treatment strategies in cervical cancer.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0259235&type=printable |
| spellingShingle | Yoko Harima Takuro Ariga Yuko Kaneyasu Hitoshi Ikushima Sunao Tokumaru Shigetoshi Shimamoto Takeo Takahashi Noriko Ii Kayoko Tsujino Anneyuko I Saito Hiroki Ushijima Takafumi Toita Tatsuya Ohno Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study. PLoS ONE |
| title | Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study. |
| title_full | Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study. |
| title_fullStr | Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study. |
| title_full_unstemmed | Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study. |
| title_short | Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated with radiation: A multicenter prospective cohort study. |
| title_sort | clinical value of serum biomarkers squamous cell carcinoma antigen and apolipoprotein c ii in follow up of patients with locally advanced cervical squamous cell carcinoma treated with radiation a multicenter prospective cohort study |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0259235&type=printable |
| work_keys_str_mv | AT yokoharima clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT takuroariga clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT yukokaneyasu clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT hitoshiikushima clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT sunaotokumaru clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT shigetoshishimamoto clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT takeotakahashi clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT norikoii clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT kayokotsujino clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT anneyukoisaito clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT hirokiushijima clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT takafumitoita clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy AT tatsuyaohno clinicalvalueofserumbiomarkerssquamouscellcarcinomaantigenandapolipoproteinciiinfollowupofpatientswithlocallyadvancedcervicalsquamouscellcarcinomatreatedwithradiationamulticenterprospectivecohortstudy |