Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential
Proper maintenance of stem cells is essential for successful utilization of ESCs/iPSCs as tools in developmental and drug discovery studies and in regenerative medicine. Standardization is critical for all future applications of stem cells and necessary to fully understand their potential. This stud...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/926463 |
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| _version_ | 1832563695952068608 |
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| author | Kristi A. Hohenstein Elliott Cory Peterson Anuradha Soundararajan Natalia Kan Brandon Nelson Sean Spiering Mark Mercola Gary R. Bright |
| author_facet | Kristi A. Hohenstein Elliott Cory Peterson Anuradha Soundararajan Natalia Kan Brandon Nelson Sean Spiering Mark Mercola Gary R. Bright |
| author_sort | Kristi A. Hohenstein Elliott |
| collection | DOAJ |
| description | Proper maintenance of stem cells is essential for successful utilization of ESCs/iPSCs as tools in developmental and drug discovery studies and in regenerative medicine. Standardization is critical for all future applications of stem cells and necessary to fully understand their potential. This study reports a novel approach for the efficient, consistent expansion of human ESCs and iPSCs using laser sectioning, instead of mechanical devices or enzymes, to divide cultures into defined size clumps for propagation. Laser-mediated propagation maintained the pluripotency, quality, and genetic stability of ESCs/iPSCs and led to enhanced differentiation potential. This approach removes the variability associated with ESC/iPSC propagation, significantly reduces the expertise, labor, and time associated with manual passaging techniques and provides the basis for scalable delivery of standardized ESC/iPSC lines. Adoption of standardized protocols would allow researchers to understand the role of genetics, environment, and/or procedural effects on stem cells and would ensure reproducible production of stem cell cultures for use in clinical/therapeutic applications. |
| format | Article |
| id | doaj-art-35f4f706deff4859bd98ab45f994e459 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-35f4f706deff4859bd98ab45f994e4592025-02-03T01:12:50ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/926463926463Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation PotentialKristi A. Hohenstein Elliott0Cory Peterson1Anuradha Soundararajan2Natalia Kan3Brandon Nelson4Sean Spiering5Mark Mercola6Gary R. Bright7Intrexon Corporation, Cell Engineering Unit, 6620 Mesa Ridge Road, San Diego, CA 92121, USAIntrexon Corporation, Cell Engineering Unit, 6620 Mesa Ridge Road, San Diego, CA 92121, USACyntellect, Inc., 6620 Mesa Ridge Road, San Diego, CA 92121, USASanford-Burnham Medical Research Institute, 10901 Torrey Pines Road, La Jolla, CA 92037, USASanford-Burnham Medical Research Institute, 10901 Torrey Pines Road, La Jolla, CA 92037, USASanford-Burnham Medical Research Institute, 10901 Torrey Pines Road, La Jolla, CA 92037, USASanford-Burnham Medical Research Institute, 10901 Torrey Pines Road, La Jolla, CA 92037, USACyntellect, Inc., 6620 Mesa Ridge Road, San Diego, CA 92121, USAProper maintenance of stem cells is essential for successful utilization of ESCs/iPSCs as tools in developmental and drug discovery studies and in regenerative medicine. Standardization is critical for all future applications of stem cells and necessary to fully understand their potential. This study reports a novel approach for the efficient, consistent expansion of human ESCs and iPSCs using laser sectioning, instead of mechanical devices or enzymes, to divide cultures into defined size clumps for propagation. Laser-mediated propagation maintained the pluripotency, quality, and genetic stability of ESCs/iPSCs and led to enhanced differentiation potential. This approach removes the variability associated with ESC/iPSC propagation, significantly reduces the expertise, labor, and time associated with manual passaging techniques and provides the basis for scalable delivery of standardized ESC/iPSC lines. Adoption of standardized protocols would allow researchers to understand the role of genetics, environment, and/or procedural effects on stem cells and would ensure reproducible production of stem cell cultures for use in clinical/therapeutic applications.http://dx.doi.org/10.1155/2012/926463 |
| spellingShingle | Kristi A. Hohenstein Elliott Cory Peterson Anuradha Soundararajan Natalia Kan Brandon Nelson Sean Spiering Mark Mercola Gary R. Bright Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential Stem Cells International |
| title | Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential |
| title_full | Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential |
| title_fullStr | Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential |
| title_full_unstemmed | Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential |
| title_short | Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential |
| title_sort | laser based propagation of human ips and es cells generates reproducible cultures with enhanced differentiation potential |
| url | http://dx.doi.org/10.1155/2012/926463 |
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