Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T Cells
Triptolide, a purified component of Tripterygiumwilfordii Hook F, has been shown to have immunosuppressive and anti-inflammatory properties in rheumatoid arthritis (RA). Although triptolide has demonstrated that it could suppress bone destruction in collagen-induced mice, its therapeutic mechanism r...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/8048170 |
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| author | Huihui Xu Hongyan Zhao Cheng Lu Qi Qiu Gui Wang Jing Huang Minghui Guo Baosheng Guo Yong Tan Cheng Xiao |
| author_facet | Huihui Xu Hongyan Zhao Cheng Lu Qi Qiu Gui Wang Jing Huang Minghui Guo Baosheng Guo Yong Tan Cheng Xiao |
| author_sort | Huihui Xu |
| collection | DOAJ |
| description | Triptolide, a purified component of Tripterygiumwilfordii Hook F, has been shown to have immunosuppressive and anti-inflammatory properties in rheumatoid arthritis (RA). Although triptolide has demonstrated that it could suppress bone destruction in collagen-induced mice, its therapeutic mechanism remains unclear. Many studies have investigated the effect of triptolide on Tregs and Tregs-related cytokine involved in RA. Additionally, previous studies have implied that Tregs inhibit osteoclast differentiation and bone resorption. Thus, in this study we aimed to explore the regulatory mechanism by which triptolide influences the Treg-mediated production of IL-10 and TGF-β1 to affect osteoclast differentiation and bone resorption. In cocultures system of Tregs and mouse bone marrow macrophages (BMMs), Tregs inhibited the differentiation of osteoclasts and reduced the resorbed areas significantly and the production of both IL-10 and TGF-β1 was upregulated. When the coculture systems were pretreated with triptolide, they produced higher levels of IL-10 and TGF-β1. Our data indicate that triptolide enhances the suppressive effects of Tregs on osteoclast differentiation and bone resorption by enhancing the secretion of IL-10 and TGF-β1. Tregs are most likely involved in the triptolide-mediated regulation of bone metabolism and may provide a potential therapeutic target for the treatment of inflammatory bone destruction. |
| format | Article |
| id | doaj-art-35f139c3dbb74973a490bb2e9998286a |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-35f139c3dbb74973a490bb2e9998286a2025-02-03T05:59:47ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/80481708048170Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T CellsHuihui Xu0Hongyan Zhao1Cheng Lu2Qi Qiu3Gui Wang4Jing Huang5Minghui Guo6Baosheng Guo7Yong Tan8Cheng Xiao9Beijing University of Chinese Medicine, Beijing 100029, ChinaInstitute of Basic Theory of Chinese Medicine, China Academy of Chinese Medical Science, Beijing 100700, ChinaInstitute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Science, Beijing 100700, ChinaInstitute of Clinical Pharmacology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaBeijing University of Chinese Medicine, Beijing 100029, ChinaInstitute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong KongInstitute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Science, Beijing 100700, ChinaInstitute of Clinical Medicine, China-Japan Friendship Hospital, Beijing 100029, ChinaTriptolide, a purified component of Tripterygiumwilfordii Hook F, has been shown to have immunosuppressive and anti-inflammatory properties in rheumatoid arthritis (RA). Although triptolide has demonstrated that it could suppress bone destruction in collagen-induced mice, its therapeutic mechanism remains unclear. Many studies have investigated the effect of triptolide on Tregs and Tregs-related cytokine involved in RA. Additionally, previous studies have implied that Tregs inhibit osteoclast differentiation and bone resorption. Thus, in this study we aimed to explore the regulatory mechanism by which triptolide influences the Treg-mediated production of IL-10 and TGF-β1 to affect osteoclast differentiation and bone resorption. In cocultures system of Tregs and mouse bone marrow macrophages (BMMs), Tregs inhibited the differentiation of osteoclasts and reduced the resorbed areas significantly and the production of both IL-10 and TGF-β1 was upregulated. When the coculture systems were pretreated with triptolide, they produced higher levels of IL-10 and TGF-β1. Our data indicate that triptolide enhances the suppressive effects of Tregs on osteoclast differentiation and bone resorption by enhancing the secretion of IL-10 and TGF-β1. Tregs are most likely involved in the triptolide-mediated regulation of bone metabolism and may provide a potential therapeutic target for the treatment of inflammatory bone destruction.http://dx.doi.org/10.1155/2016/8048170 |
| spellingShingle | Huihui Xu Hongyan Zhao Cheng Lu Qi Qiu Gui Wang Jing Huang Minghui Guo Baosheng Guo Yong Tan Cheng Xiao Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T Cells Mediators of Inflammation |
| title | Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T Cells |
| title_full | Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T Cells |
| title_fullStr | Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T Cells |
| title_full_unstemmed | Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T Cells |
| title_short | Triptolide Inhibits Osteoclast Differentiation and Bone Resorption In Vitro via Enhancing the Production of IL-10 and TGF-β1 by Regulatory T Cells |
| title_sort | triptolide inhibits osteoclast differentiation and bone resorption in vitro via enhancing the production of il 10 and tgf β1 by regulatory t cells |
| url | http://dx.doi.org/10.1155/2016/8048170 |
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