Silencing RNA for MMPs May Be Utilized for Cardioprotection
Ischemia/reperfusion (I/R) injury is accompanied by an increase of matrix metalloproteinase 2 (MMP-2) activity, which degrades heart contractile proteins. The aim of the study was to investigate the effect of MMP-2 small interfering RNA (MMP-2 siRNA) administration on I/R heart. Isolated rat hearts...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2022/9729018 |
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| author | Marta Banaszkiewicz Anna Krzywonos-Zawadzka Agnieszka Olejnik Agnieszka Noszczyk-Nowak Iwona Bil-Lula |
| author_facet | Marta Banaszkiewicz Anna Krzywonos-Zawadzka Agnieszka Olejnik Agnieszka Noszczyk-Nowak Iwona Bil-Lula |
| author_sort | Marta Banaszkiewicz |
| collection | DOAJ |
| description | Ischemia/reperfusion (I/R) injury is accompanied by an increase of matrix metalloproteinase 2 (MMP-2) activity, which degrades heart contractile proteins. The aim of the study was to investigate the effect of MMP-2 small interfering RNA (MMP-2 siRNA) administration on I/R heart. Isolated rat hearts perfused by the Langendorff method were subjected to I/R in the presence or absence of MMP-2 siRNA. The hemodynamic parameters of heart function were monitored. Lactate dehydrogenase (LDH) activity was measured in coronary effluents. Activity and concentration of MMPs in the hearts were measured. Concentration of troponin I (TnI) in coronary effluents was examined as a target for MMP-2 degradation. Recovery of heart mechanical function was reduced after I/R; however, administration of MMP-2 siRNA resulted in restoration of proper mechanical function (p<0.001). LDH activity was decreased after the use of MMP-2 siRNA (p=0.02), providing evidence for reduced cardiac damage. Both MMP-2 and MMP-9 syntheses as well as their activity were inhibited in the I/R hearts after siRNA administration (p<0.05). MMP-2 siRNA administration inhibited TnI release into the coronary effluents (p<0.001). The use of MMP-2 siRNA contributed to the improvement of heart mechanical function and reduction of contractile proteins degradation during I/R; therefore, MMP-2 siRNA may be considered a cardioprotective agent. |
| format | Article |
| id | doaj-art-35e7a5abcc134e20aa16e96f8151e994 |
| institution | Kabale University |
| issn | 1755-5922 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-35e7a5abcc134e20aa16e96f8151e9942025-02-03T05:57:23ZengWileyCardiovascular Therapeutics1755-59222022-01-01202210.1155/2022/9729018Silencing RNA for MMPs May Be Utilized for CardioprotectionMarta Banaszkiewicz0Anna Krzywonos-Zawadzka1Agnieszka Olejnik2Agnieszka Noszczyk-Nowak3Iwona Bil-Lula4Division of Clinical Chemistry and Laboratory HematologyDivision of Clinical Chemistry and Laboratory HematologyDivision of Clinical Chemistry and Laboratory HematologyDepartment of Internal Medicine and Clinic of Diseases of HorsesDivision of Clinical Chemistry and Laboratory HematologyIschemia/reperfusion (I/R) injury is accompanied by an increase of matrix metalloproteinase 2 (MMP-2) activity, which degrades heart contractile proteins. The aim of the study was to investigate the effect of MMP-2 small interfering RNA (MMP-2 siRNA) administration on I/R heart. Isolated rat hearts perfused by the Langendorff method were subjected to I/R in the presence or absence of MMP-2 siRNA. The hemodynamic parameters of heart function were monitored. Lactate dehydrogenase (LDH) activity was measured in coronary effluents. Activity and concentration of MMPs in the hearts were measured. Concentration of troponin I (TnI) in coronary effluents was examined as a target for MMP-2 degradation. Recovery of heart mechanical function was reduced after I/R; however, administration of MMP-2 siRNA resulted in restoration of proper mechanical function (p<0.001). LDH activity was decreased after the use of MMP-2 siRNA (p=0.02), providing evidence for reduced cardiac damage. Both MMP-2 and MMP-9 syntheses as well as their activity were inhibited in the I/R hearts after siRNA administration (p<0.05). MMP-2 siRNA administration inhibited TnI release into the coronary effluents (p<0.001). The use of MMP-2 siRNA contributed to the improvement of heart mechanical function and reduction of contractile proteins degradation during I/R; therefore, MMP-2 siRNA may be considered a cardioprotective agent.http://dx.doi.org/10.1155/2022/9729018 |
| spellingShingle | Marta Banaszkiewicz Anna Krzywonos-Zawadzka Agnieszka Olejnik Agnieszka Noszczyk-Nowak Iwona Bil-Lula Silencing RNA for MMPs May Be Utilized for Cardioprotection Cardiovascular Therapeutics |
| title | Silencing RNA for MMPs May Be Utilized for Cardioprotection |
| title_full | Silencing RNA for MMPs May Be Utilized for Cardioprotection |
| title_fullStr | Silencing RNA for MMPs May Be Utilized for Cardioprotection |
| title_full_unstemmed | Silencing RNA for MMPs May Be Utilized for Cardioprotection |
| title_short | Silencing RNA for MMPs May Be Utilized for Cardioprotection |
| title_sort | silencing rna for mmps may be utilized for cardioprotection |
| url | http://dx.doi.org/10.1155/2022/9729018 |
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