Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot study
Objectives: Biomimetic remineralization strategies offer promising paths for managing caries- affected dentin by restoring its morphology and structural integrity. This pilot study aimed to assess the morphological and structural changes in dentin following two chemical artificial caries-affected de...
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Sociedade Portuguesa de Estomatologia e Medicina Dentária
2024-12-01
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| Series: | Revista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial |
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| Online Access: | https://administracao.spemd.pt/app/assets/imagens/files_img/1_19_6788e5f2a523e.pdf |
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| author | Rosário Costa Sofia Arantes-Oliveira Lisete Fernandes Fábio G. Figueiras João Cardoso Ferreira Paulo Ribeiro de Melo |
| author_facet | Rosário Costa Sofia Arantes-Oliveira Lisete Fernandes Fábio G. Figueiras João Cardoso Ferreira Paulo Ribeiro de Melo |
| author_sort | Rosário Costa |
| collection | DOAJ |
| description | Objectives: Biomimetic remineralization strategies offer promising paths for managing caries- affected dentin by restoring its morphology and structural integrity. This pilot study aimed to assess the morphological and structural changes in dentin following two chemical artificial caries-affected dentin (ACAD) protocols, evaluate the effects of three biomimetic remineralization procedures (BRPs) on dentin structure, and determine the most effective ACAD-BRP combination. The BRPs studied were casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), self-assembling P11-4 (SAP-P11-4), and calcium phosphate polymer- induced liquid precursor (Ca/P-PILP). Methods: Seventy-two specimens of healthy human dentin were divided into twelve groups. Half of each group’s samples were analyzed using field-emission gun environmental scanning electron microscope/energy dispersive X-ray, while the other half were analyzed by X-ray diffraction and micro-Raman spectroscopy. Results: Results demonstrate alterations in dentin morphology and structure induced by the ACAD protocols, affirming their effectiveness in mimicking caries-affected dentin. Among the BRPs evaluated, CPP-ACP emerged as the most promising agent for promoting remineralization and restoring dentin structure. The combination of pH cycling with CPP-ACP exhibited superior efficacy in promoting dentin remineralization compared to other combinations. Conclusions: These findings highlight the potential of biomimetic remineralization strategies, particularly CPP-ACP combined with pH cycling, as effective approaches for managing caries-affected dentin and preserving dental health. |
| format | Article |
| id | doaj-art-35df83f0c71b4150a8b1bbf9576e0e23 |
| institution | Kabale University |
| issn | 1646-2890 1647-6700 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sociedade Portuguesa de Estomatologia e Medicina Dentária |
| record_format | Article |
| series | Revista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial |
| spelling | doaj-art-35df83f0c71b4150a8b1bbf9576e0e232025-01-28T10:52:50ZengSociedade Portuguesa de Estomatologia e Medicina DentáriaRevista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial1646-28901647-67002024-12-0165418819610.24873/j.rpemd.2024.12.1414Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot studyRosário Costa0https://orcid.org/0000-0003-1022-6858Sofia Arantes-Oliveira1https://orcid.org/0000-0001-9105-0975Lisete Fernandes2https://orcid.org/0000-0002-2986-7818Fábio G. Figueiras3https://orcid.org/0000-0001-7841-9731João Cardoso Ferreira4https://orcid.org/0000-0003-4575-4861Paulo Ribeiro de Melo5https://orcid.org/0000-0003-3590-4926Departamento de Dentisteria, Faculdade de Medicina Dentária, Universidade do Porto, Porto, PortugalDepartamento de Biomateriais, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisbon, PortugalUnidade de Microscopia Eletrónica, Universidade de Trás-os-Montes e Alto Douro, Vila Real, PortugalDepartamento de Física e Astronomia, Faculdade de Ciências, Universidade do Porto, Porto, PortugalDepartamento de Dentisteria, Faculdade de Medicina Dentária, Universidade do Porto, Porto, PortugalDepartamento de Dentisteria, Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal; EpiUnit, ITR, Instituto de Saúde Pública, Faculdade de Medicina Dentária, Universidade do Porto, Porto, PortugalObjectives: Biomimetic remineralization strategies offer promising paths for managing caries- affected dentin by restoring its morphology and structural integrity. This pilot study aimed to assess the morphological and structural changes in dentin following two chemical artificial caries-affected dentin (ACAD) protocols, evaluate the effects of three biomimetic remineralization procedures (BRPs) on dentin structure, and determine the most effective ACAD-BRP combination. The BRPs studied were casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), self-assembling P11-4 (SAP-P11-4), and calcium phosphate polymer- induced liquid precursor (Ca/P-PILP). Methods: Seventy-two specimens of healthy human dentin were divided into twelve groups. Half of each group’s samples were analyzed using field-emission gun environmental scanning electron microscope/energy dispersive X-ray, while the other half were analyzed by X-ray diffraction and micro-Raman spectroscopy. Results: Results demonstrate alterations in dentin morphology and structure induced by the ACAD protocols, affirming their effectiveness in mimicking caries-affected dentin. Among the BRPs evaluated, CPP-ACP emerged as the most promising agent for promoting remineralization and restoring dentin structure. The combination of pH cycling with CPP-ACP exhibited superior efficacy in promoting dentin remineralization compared to other combinations. Conclusions: These findings highlight the potential of biomimetic remineralization strategies, particularly CPP-ACP combined with pH cycling, as effective approaches for managing caries-affected dentin and preserving dental health.https://administracao.spemd.pt/app/assets/imagens/files_img/1_19_6788e5f2a523e.pdfbiomimeticsdemineralized dentin matrixdentintooth remineralization |
| spellingShingle | Rosário Costa Sofia Arantes-Oliveira Lisete Fernandes Fábio G. Figueiras João Cardoso Ferreira Paulo Ribeiro de Melo Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot study Revista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial biomimetics demineralized dentin matrix dentin tooth remineralization |
| title | Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot study |
| title_full | Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot study |
| title_fullStr | Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot study |
| title_full_unstemmed | Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot study |
| title_short | Effectiveness of three biomimetic remineralization procedures in artificial caries-affected dentin – an in-vitro pilot study |
| title_sort | effectiveness of three biomimetic remineralization procedures in artificial caries affected dentin an in vitro pilot study |
| topic | biomimetics demineralized dentin matrix dentin tooth remineralization |
| url | https://administracao.spemd.pt/app/assets/imagens/files_img/1_19_6788e5f2a523e.pdf |
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