Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts
Objective. The transverse-axial tubule system (TATS) of cardiomyocytes allows a spatially coordinated conversion of electrical excitation into an intracellular Ca2+ signal and consequently contraction. Previous reports have indicated alterations of structure and/or volume of the TATS in cardiac hype...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.4061/2009/802373 |
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| author | Andreas Ohler Jutta Weisser-Thomas Valentino Piacentino Steven R. Houser Gordon F. Tomaselli Brian O'Rourke |
| author_facet | Andreas Ohler Jutta Weisser-Thomas Valentino Piacentino Steven R. Houser Gordon F. Tomaselli Brian O'Rourke |
| author_sort | Andreas Ohler |
| collection | DOAJ |
| description | Objective. The transverse-axial tubule system (TATS) of cardiomyocytes allows a spatially coordinated conversion of electrical excitation into an intracellular Ca2+ signal and consequently contraction. Previous reports have indicated alterations of structure and/or volume of the TATS in cardiac hypertrophy and failure, suggesting a contribution to the impairment of excitation contraction coupling. To test whether structural alterations are present in human heart failure, the TATS was visualized in myocytes from failing and non-failing human hearts. Methods and Results. In freshly isolated myocytes, the plasmalemmal membranes were labeled with Di-8-ANEPPS and imaged using two-photon excitation at 780 nm. Optical sections were taken every 300 nm through the cells. After deconvolution, the TATS was determined within the 3D data sets, revealing no significant difference in normalized surface area or volume. To rule out possible inhomogeneity in the arrangement of the TATS, Euclidian distance maps were plotted for every section, allowing to measure the closest distance between any cytosolic and any membrane point. There was a trend towards greater spacing in cells from failing hearts, without statistical significance. Conclusion. Only small changes, but no significant changes in the geometrical dimensions of the TATS were observed in cardiomyocytes from failing compared to non-failing human myocardium. |
| format | Article |
| id | doaj-art-35df829070974ba282f0501b4b7f130d |
| institution | Kabale University |
| issn | 2090-0597 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
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| series | Cardiology Research and Practice |
| spelling | doaj-art-35df829070974ba282f0501b4b7f130d2025-02-03T01:02:51ZengWileyCardiology Research and Practice2090-05972009-01-01200910.4061/2009/802373802373Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human HeartsAndreas Ohler0Jutta Weisser-Thomas1Valentino Piacentino2Steven R. Houser3Gordon F. Tomaselli4Brian O'Rourke5Department of Medicine, Institute of Molecular Cardiobiology, Johns Hopkins University, Baltimore, MD 21205, USADepartment of Physiology, Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USADepartment of Physiology, Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USADepartment of Physiology, Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USADepartment of Medicine, Institute of Molecular Cardiobiology, Johns Hopkins University, Baltimore, MD 21205, USADepartment of Medicine, Institute of Molecular Cardiobiology, Johns Hopkins University, Baltimore, MD 21205, USAObjective. The transverse-axial tubule system (TATS) of cardiomyocytes allows a spatially coordinated conversion of electrical excitation into an intracellular Ca2+ signal and consequently contraction. Previous reports have indicated alterations of structure and/or volume of the TATS in cardiac hypertrophy and failure, suggesting a contribution to the impairment of excitation contraction coupling. To test whether structural alterations are present in human heart failure, the TATS was visualized in myocytes from failing and non-failing human hearts. Methods and Results. In freshly isolated myocytes, the plasmalemmal membranes were labeled with Di-8-ANEPPS and imaged using two-photon excitation at 780 nm. Optical sections were taken every 300 nm through the cells. After deconvolution, the TATS was determined within the 3D data sets, revealing no significant difference in normalized surface area or volume. To rule out possible inhomogeneity in the arrangement of the TATS, Euclidian distance maps were plotted for every section, allowing to measure the closest distance between any cytosolic and any membrane point. There was a trend towards greater spacing in cells from failing hearts, without statistical significance. Conclusion. Only small changes, but no significant changes in the geometrical dimensions of the TATS were observed in cardiomyocytes from failing compared to non-failing human myocardium.http://dx.doi.org/10.4061/2009/802373 |
| spellingShingle | Andreas Ohler Jutta Weisser-Thomas Valentino Piacentino Steven R. Houser Gordon F. Tomaselli Brian O'Rourke Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts Cardiology Research and Practice |
| title | Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts |
| title_full | Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts |
| title_fullStr | Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts |
| title_full_unstemmed | Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts |
| title_short | Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts |
| title_sort | two photon laser scanning microscopy of the transverse axial tubule system in ventricular cardiomyocytes from failing and non failing human hearts |
| url | http://dx.doi.org/10.4061/2009/802373 |
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