Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopy
Abstract Phloem-feeding insects present significant economic threats worldwide and remain challenging to understand due to their specialized feeding strategies. Significant advances in genetics, genomics, and biochemistry have greatly enriched our comprehension of phloem–insect interactions. However...
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Nature Portfolio
2025-03-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07865-8 |
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| author | Guan Wang Jian-Sheng Guo Hai-Jian Huang Zeng-Rong Zhu Chuan-Xi Zhang |
| author_facet | Guan Wang Jian-Sheng Guo Hai-Jian Huang Zeng-Rong Zhu Chuan-Xi Zhang |
| author_sort | Guan Wang |
| collection | DOAJ |
| description | Abstract Phloem-feeding insects present significant economic threats worldwide and remain challenging to understand due to their specialized feeding strategies. Significant advances in genetics, genomics, and biochemistry have greatly enriched our comprehension of phloem–insect interactions. However, existing studies relying on two-dimensional discrete images have limited our understanding of visible morphological details. In this study, we leverage volume electron microscopy (vEM) technology to unveil a nanometer-resolution interaction mode between plant and the phloem-feeding insect, Camphor psyllid (Trioza camphorae, Hemiptera: Psyllidae). The stylets penetrate each cell on the way to the feeding site (sieve tube), and new cell walls will form around the salivary sheath, ultimately fusing with the original cell walls to form remarkably thickening cell walls. Our reconstruction findings on pit gall tissues suggest that a significant decrease in cell volume and a drastic increase in cell layers are the primary processes during pit gall formation. These unique findings will set the stage for a robust discussion on the plant cellular response induced by phloem-feeding insects. |
| format | Article |
| id | doaj-art-35c7f61d814b4a7f80c34b518f5275f6 |
| institution | DOAJ |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-35c7f61d814b4a7f80c34b518f5275f62025-08-20T02:41:33ZengNature PortfolioCommunications Biology2399-36422025-03-018111510.1038/s42003-025-07865-8Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopyGuan Wang0Jian-Sheng Guo1Hai-Jian Huang2Zeng-Rong Zhu3Chuan-Xi Zhang4Institute of Insect Science, Zhejiang UniversityCenter of Cryo-Electron Microscopy, School of Medicine, Zhejiang UniversityState Key Laboratory for Quality and Safety of Agro-Products, Ningbo UniversityInstitute of Insect Science, Zhejiang UniversityInstitute of Insect Science, Zhejiang UniversityAbstract Phloem-feeding insects present significant economic threats worldwide and remain challenging to understand due to their specialized feeding strategies. Significant advances in genetics, genomics, and biochemistry have greatly enriched our comprehension of phloem–insect interactions. However, existing studies relying on two-dimensional discrete images have limited our understanding of visible morphological details. In this study, we leverage volume electron microscopy (vEM) technology to unveil a nanometer-resolution interaction mode between plant and the phloem-feeding insect, Camphor psyllid (Trioza camphorae, Hemiptera: Psyllidae). The stylets penetrate each cell on the way to the feeding site (sieve tube), and new cell walls will form around the salivary sheath, ultimately fusing with the original cell walls to form remarkably thickening cell walls. Our reconstruction findings on pit gall tissues suggest that a significant decrease in cell volume and a drastic increase in cell layers are the primary processes during pit gall formation. These unique findings will set the stage for a robust discussion on the plant cellular response induced by phloem-feeding insects.https://doi.org/10.1038/s42003-025-07865-8 |
| spellingShingle | Guan Wang Jian-Sheng Guo Hai-Jian Huang Zeng-Rong Zhu Chuan-Xi Zhang Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopy Communications Biology |
| title | Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopy |
| title_full | Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopy |
| title_fullStr | Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopy |
| title_full_unstemmed | Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopy |
| title_short | Three-dimensional interaction between Cinnamomum camphora and a sap-sucking psyllid insect (Trioza camphorae) revealed by nano-resolution volume electron microscopy |
| title_sort | three dimensional interaction between cinnamomum camphora and a sap sucking psyllid insect trioza camphorae revealed by nano resolution volume electron microscopy |
| url | https://doi.org/10.1038/s42003-025-07865-8 |
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