Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells
Objective. This study aimed to explore the immunoregulatory effect of flavonoids of blueberry (Vaccinium corymbosum L.) leaves (FBL). Methods. The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mas...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/5476903 |
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| _version_ | 1832555830145187840 |
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| author | Dazhi Shi Mengyi Xu Mengyue Ren Enshan Pan Chaohua Luo Wei Zhang Qingfa Tang |
| author_facet | Dazhi Shi Mengyi Xu Mengyue Ren Enshan Pan Chaohua Luo Wei Zhang Qingfa Tang |
| author_sort | Dazhi Shi |
| collection | DOAJ |
| description | Objective. This study aimed to explore the immunoregulatory effect of flavonoids of blueberry (Vaccinium corymbosum L.) leaves (FBL). Methods. The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of FBL were investigated in lipopolysaccharide- (LPS-) induced RAW 264.7 cells. Results. According to the results of UPLC/Q-Tof-MS, nine flavonoids of blueberry leaves were identified. FBL showed a significant reduction in the production of TNF-α in LPS-stimulated RAW 264.7 cells. FBL significantly decreased the expression of NF-κB p65 and P-NF-κB p65 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Conclusion. Our study showed the immunoregulatory effect of FBL through the suppression of TNF-α via the NF-κB signal pathway. |
| format | Article |
| id | doaj-art-35b822dc065c4ca5b0b63fc7b5b8113d |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-35b822dc065c4ca5b0b63fc7b5b8113d2025-02-03T05:47:06ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/54769035476903Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 CellsDazhi Shi0Mengyi Xu1Mengyue Ren2Enshan Pan3Chaohua Luo4Wei Zhang5Qingfa Tang6School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaState Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, MacauSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, ChinaObjective. This study aimed to explore the immunoregulatory effect of flavonoids of blueberry (Vaccinium corymbosum L.) leaves (FBL). Methods. The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of FBL were investigated in lipopolysaccharide- (LPS-) induced RAW 264.7 cells. Results. According to the results of UPLC/Q-Tof-MS, nine flavonoids of blueberry leaves were identified. FBL showed a significant reduction in the production of TNF-α in LPS-stimulated RAW 264.7 cells. FBL significantly decreased the expression of NF-κB p65 and P-NF-κB p65 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Conclusion. Our study showed the immunoregulatory effect of FBL through the suppression of TNF-α via the NF-κB signal pathway.http://dx.doi.org/10.1155/2017/5476903 |
| spellingShingle | Dazhi Shi Mengyi Xu Mengyue Ren Enshan Pan Chaohua Luo Wei Zhang Qingfa Tang Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells Journal of Immunology Research |
| title | Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells |
| title_full | Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells |
| title_fullStr | Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells |
| title_full_unstemmed | Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells |
| title_short | Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells |
| title_sort | immunomodulatory effect of flavonoids of blueberry vaccinium corymbosum l leaves via the nf κb signal pathway in lps stimulated raw 264 7 cells |
| url | http://dx.doi.org/10.1155/2017/5476903 |
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