Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria
Background. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, and Staphylococcus aureus. Methods. Aqueous and ethanol extracts of Ju...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2013/854765 |
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| author | Faramarz Zakavi Leila Golpasand Hagh Arash Daraeighadikolaei Ahmad Farajzadeh Sheikh Arsham Daraeighadikolaei Zahra Leilavi Shooshtari |
| author_facet | Faramarz Zakavi Leila Golpasand Hagh Arash Daraeighadikolaei Ahmad Farajzadeh Sheikh Arsham Daraeighadikolaei Zahra Leilavi Shooshtari |
| author_sort | Faramarz Zakavi |
| collection | DOAJ |
| description | Background. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, and Staphylococcus aureus.
Methods. Aqueous and ethanol extracts of Juglans regia bark were prepared by using disk diffusion technique and Minimal Inhibitory Concentration (MIC) methods. Tetracycline 30 μg and Erythromycin 15 μg were used as positive control and water as negative control in disk diffusion and MIC methods. Data were analyzed by ANOVA test.
Results. The results showed that S. sanguis and S. mutans were the most sensitive and the most resistant bacteria against ethanolic and aqueous extracts, respectively. Ethanolic extract had significant antibacterial effect against all tested bacteria. Aqueous extract did not show antibacterial effect on S. mutans, in contrast to ethanolic extract. Aqueous extract had significantly antibacterial effect against Staphylococcus aureus, S. salivarius, and S. sanguis compared to control (P<0.0001), but it did not show effect on S. mutans when compared with Erythromycin. According to the obtained MIC values, ethanol extract of Juglans regia bark had the lowest rate.
Conclusion. The results may provide the basis for using natural antimicrobial substance for oral hygiene prophylaxis purposes. |
| format | Article |
| id | doaj-art-35aedad9a1e84c2a9aca20ea88aeab40 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-35aedad9a1e84c2a9aca20ea88aeab402025-02-03T01:20:14ZengWileyInternational Journal of Dentistry1687-87281687-87362013-01-01201310.1155/2013/854765854765Antibacterial Effect of Juglans Regia Bark against Oral Pathologic BacteriaFaramarz Zakavi0Leila Golpasand Hagh1Arash Daraeighadikolaei2Ahmad Farajzadeh Sheikh3Arsham Daraeighadikolaei4Zahra Leilavi Shooshtari5Department of Operative and Esthetic Dentistry, School of Dentistry, Ahvaz Jundishapour University of Medical Sciences, Ahvaz 613571-5775, IranDepartment of Periodontology, School of Dentistry, Ahvaz Jundishapour University of Medical Sciences, Ahvaz 613571-5775, IranAhvaz Dental School Research Center, Ahvaz 613571-5775, IranDepartment of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61355-45, IranDepartment of Pharmacognosy Clinical, Kerman School of Pharmacy, Kerman 76135-1614, IranAhvaz Dental School Research Center, Ahvaz 613571-5775, IranBackground. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, and Staphylococcus aureus. Methods. Aqueous and ethanol extracts of Juglans regia bark were prepared by using disk diffusion technique and Minimal Inhibitory Concentration (MIC) methods. Tetracycline 30 μg and Erythromycin 15 μg were used as positive control and water as negative control in disk diffusion and MIC methods. Data were analyzed by ANOVA test. Results. The results showed that S. sanguis and S. mutans were the most sensitive and the most resistant bacteria against ethanolic and aqueous extracts, respectively. Ethanolic extract had significant antibacterial effect against all tested bacteria. Aqueous extract did not show antibacterial effect on S. mutans, in contrast to ethanolic extract. Aqueous extract had significantly antibacterial effect against Staphylococcus aureus, S. salivarius, and S. sanguis compared to control (P<0.0001), but it did not show effect on S. mutans when compared with Erythromycin. According to the obtained MIC values, ethanol extract of Juglans regia bark had the lowest rate. Conclusion. The results may provide the basis for using natural antimicrobial substance for oral hygiene prophylaxis purposes.http://dx.doi.org/10.1155/2013/854765 |
| spellingShingle | Faramarz Zakavi Leila Golpasand Hagh Arash Daraeighadikolaei Ahmad Farajzadeh Sheikh Arsham Daraeighadikolaei Zahra Leilavi Shooshtari Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria International Journal of Dentistry |
| title | Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria |
| title_full | Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria |
| title_fullStr | Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria |
| title_full_unstemmed | Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria |
| title_short | Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria |
| title_sort | antibacterial effect of juglans regia bark against oral pathologic bacteria |
| url | http://dx.doi.org/10.1155/2013/854765 |
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