Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder
Objective. To determine potential mitochondrial and oxidative alterations in colon biopsies from idiopathic REM sleep behavior disorder (iRBD) and Parkinson’s disease (PD) subjects. Methods. Colonic biopsies from 7 iRBD subjects, 9 subjects with clinically diagnosed PD, and 9 healthy controls were h...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Parkinson's Disease |
| Online Access: | http://dx.doi.org/10.1155/2017/9816095 |
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| author | C. Morén Í. González-Casacuberta J. Navarro-Otano D. Juárez-Flores D. Vilas G. Garrabou J. C. Milisenda C. Pont-Sunyer M. Catalán-García M. Guitart-Mampel E. Tobías F. Cardellach F. Valldeoriola A. Iranzo E. Tolosa |
| author_facet | C. Morén Í. González-Casacuberta J. Navarro-Otano D. Juárez-Flores D. Vilas G. Garrabou J. C. Milisenda C. Pont-Sunyer M. Catalán-García M. Guitart-Mampel E. Tobías F. Cardellach F. Valldeoriola A. Iranzo E. Tolosa |
| author_sort | C. Morén |
| collection | DOAJ |
| description | Objective. To determine potential mitochondrial and oxidative alterations in colon biopsies from idiopathic REM sleep behavior disorder (iRBD) and Parkinson’s disease (PD) subjects. Methods. Colonic biopsies from 7 iRBD subjects, 9 subjects with clinically diagnosed PD, and 9 healthy controls were homogenized in 5% w/v mannitol. Citrate synthase (CS) and complex I (CI) were analyzed spectrophotometrically. Oxidative damage was assessed either by lipid peroxidation, through malondialdehyde and hydroxyalkenal content by spectrophotometry, or through antioxidant enzyme levels of superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (Gpx1), and catalase (CAT) by western blot. The presence of mitochondrial DNA (mtDNA) deletions was assessed by long PCR and electrophoresis. Results. Nonsignificant trends to CI decrease in both iRBD (45.69±18.15; 23% decrease) and PD patients (37.57±12.41; 37% decrease) were found compared to controls (59.51±12.52, p: NS). Lipid peroxidation was maintained among groups (iRBD: 27.46±3.04, PD: 37.2±3.92, and controls: 31.71±3.94; p: NS). Antioxidant enzymes SOD2 (iRBD: 2.30±0.92, PD: 1.48±0.39, and controls: 1.09±0.318) and Gpx1 (iRBD 0.29±0.12, PD: 0.56±0.33, and controls: 0.38±0.16) did not show significant differences between groups. CAT was only detected in 2 controls and 1 iRBD subject. One iRBD patient presented a single mtDNA deletion. |
| format | Article |
| id | doaj-art-35a9ccc883204c7b9b613b0ec08f4f16 |
| institution | Kabale University |
| issn | 2090-8083 2042-0080 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Parkinson's Disease |
| spelling | doaj-art-35a9ccc883204c7b9b613b0ec08f4f162025-02-03T06:01:11ZengWileyParkinson's Disease2090-80832042-00802017-01-01201710.1155/2017/98160959816095Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior DisorderC. Morén0Í. González-Casacuberta1J. Navarro-Otano2D. Juárez-Flores3D. Vilas4G. Garrabou5J. C. Milisenda6C. Pont-Sunyer7M. Catalán-García8M. Guitart-Mampel9E. Tobías10F. Cardellach11F. Valldeoriola12A. Iranzo13E. Tolosa14Muscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainNeurology Service, Hospital Clínic of Barcelona, IDIBAPS, CIBERNED, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMovement Disorder Unit, Neurology Service, Hospital Clínic of Barcelona, IDIBAPS, CIBERNED, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMovement Disorder Unit, Neurology Service, Hospital Clínic of Barcelona, IDIBAPS, CIBERNED, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMuscle Research and Mitochondrial Function Laboratory, Cellex-IDIBAPS, Faculty of Medicine and Health Sciences, University of Barcelona, Internal Medicine Department, Hospital Clínic of Barcelona, CIBERER U722, Barcelona, SpainMovement Disorder Unit, Neurology Service, Hospital Clínic of Barcelona, IDIBAPS, CIBERNED, Barcelona, SpainNeurology Service, Hospital Clínic of Barcelona, IDIBAPS, CIBERNED, Barcelona, SpainMovement Disorder Unit, Neurology Service, Hospital Clínic of Barcelona, IDIBAPS, CIBERNED, Barcelona, SpainObjective. To determine potential mitochondrial and oxidative alterations in colon biopsies from idiopathic REM sleep behavior disorder (iRBD) and Parkinson’s disease (PD) subjects. Methods. Colonic biopsies from 7 iRBD subjects, 9 subjects with clinically diagnosed PD, and 9 healthy controls were homogenized in 5% w/v mannitol. Citrate synthase (CS) and complex I (CI) were analyzed spectrophotometrically. Oxidative damage was assessed either by lipid peroxidation, through malondialdehyde and hydroxyalkenal content by spectrophotometry, or through antioxidant enzyme levels of superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (Gpx1), and catalase (CAT) by western blot. The presence of mitochondrial DNA (mtDNA) deletions was assessed by long PCR and electrophoresis. Results. Nonsignificant trends to CI decrease in both iRBD (45.69±18.15; 23% decrease) and PD patients (37.57±12.41; 37% decrease) were found compared to controls (59.51±12.52, p: NS). Lipid peroxidation was maintained among groups (iRBD: 27.46±3.04, PD: 37.2±3.92, and controls: 31.71±3.94; p: NS). Antioxidant enzymes SOD2 (iRBD: 2.30±0.92, PD: 1.48±0.39, and controls: 1.09±0.318) and Gpx1 (iRBD 0.29±0.12, PD: 0.56±0.33, and controls: 0.38±0.16) did not show significant differences between groups. CAT was only detected in 2 controls and 1 iRBD subject. One iRBD patient presented a single mtDNA deletion.http://dx.doi.org/10.1155/2017/9816095 |
| spellingShingle | C. Morén Í. González-Casacuberta J. Navarro-Otano D. Juárez-Flores D. Vilas G. Garrabou J. C. Milisenda C. Pont-Sunyer M. Catalán-García M. Guitart-Mampel E. Tobías F. Cardellach F. Valldeoriola A. Iranzo E. Tolosa Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder Parkinson's Disease |
| title | Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder |
| title_full | Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder |
| title_fullStr | Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder |
| title_full_unstemmed | Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder |
| title_short | Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder |
| title_sort | colonic oxidative and mitochondrial function in parkinson s disease and idiopathic rem sleep behavior disorder |
| url | http://dx.doi.org/10.1155/2017/9816095 |
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