Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers
We report a nanoscale replication method suitable for biological specimens that has potential in single cell studies and in formation of 3D biocompatible scaffolds. Earlier studies using a heat-curable polydimethylsiloxane (PDMS) or a UV-curable elastomer introduced Bioimprint replication to facilit...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2009/593410 |
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| author | Fahmi Samsuri John S. Mitchell Maan M. Alkaisi John J. Evans |
| author_facet | Fahmi Samsuri John S. Mitchell Maan M. Alkaisi John J. Evans |
| author_sort | Fahmi Samsuri |
| collection | DOAJ |
| description | We report a nanoscale replication method suitable for biological specimens that has potential in single cell studies and in formation of 3D biocompatible scaffolds. Earlier studies using a heat-curable polydimethylsiloxane (PDMS) or a UV-curable elastomer introduced Bioimprint replication to facilitate cell imaging. However, the replicating conditions for thermal polymerization are known to cause cell dehydration during curing. In this study, a UV-cured methacrylate copolymer was developed for use in creating replicas of living cells and was tested on rat muscle cells. Bioimprints of muscle cells were formed by spin coating under UV irradiation. The polymer replicas were then separated from the muscle cells and were analyzed under an Atomic Force Microscope (AFM), in tapping mode, because it has low tip-sample forces and thus will not destroy the fine structures of the imprint. The new polymer is biocompatible with higher replication resolution and has a faster curing process than other types of silicon-based organic polymers such as PDMS. High resolution images of the muscle cell imprints showed the micro-and nanostructures of the muscle cells, including cellular fibers and structures within the cell membranes. The AFM is able to image features at nanoscale resolution with the potential for recognizing abnormalities on cell membranes at early stages of disease progression. |
| format | Article |
| id | doaj-art-358ceec06ec64cad884164c33749e293 |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-358ceec06ec64cad884164c33749e2932025-02-03T01:22:33ZengWileyJournal of Nanotechnology1687-95031687-95112009-01-01200910.1155/2009/593410593410Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated PolymersFahmi Samsuri0John S. Mitchell1Maan M. Alkaisi2John J. Evans3Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8140, New ZealandThe New Zealand Institute for Plant & Food Research Ltd, Hamilton 3214, New ZealandDepartment of Electrical and Computer Engineering, University of Canterbury, Christchurch 8140, New ZealandCentre for Neuroendocrinology and Christchurch School of Medicine and Health Sciences, University of Otago, Christchurch 8140, New ZealandWe report a nanoscale replication method suitable for biological specimens that has potential in single cell studies and in formation of 3D biocompatible scaffolds. Earlier studies using a heat-curable polydimethylsiloxane (PDMS) or a UV-curable elastomer introduced Bioimprint replication to facilitate cell imaging. However, the replicating conditions for thermal polymerization are known to cause cell dehydration during curing. In this study, a UV-cured methacrylate copolymer was developed for use in creating replicas of living cells and was tested on rat muscle cells. Bioimprints of muscle cells were formed by spin coating under UV irradiation. The polymer replicas were then separated from the muscle cells and were analyzed under an Atomic Force Microscope (AFM), in tapping mode, because it has low tip-sample forces and thus will not destroy the fine structures of the imprint. The new polymer is biocompatible with higher replication resolution and has a faster curing process than other types of silicon-based organic polymers such as PDMS. High resolution images of the muscle cell imprints showed the micro-and nanostructures of the muscle cells, including cellular fibers and structures within the cell membranes. The AFM is able to image features at nanoscale resolution with the potential for recognizing abnormalities on cell membranes at early stages of disease progression.http://dx.doi.org/10.1155/2009/593410 |
| spellingShingle | Fahmi Samsuri John S. Mitchell Maan M. Alkaisi John J. Evans Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers Journal of Nanotechnology |
| title | Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers |
| title_full | Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers |
| title_fullStr | Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers |
| title_full_unstemmed | Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers |
| title_short | Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers |
| title_sort | formation of nanoscale bioimprints of muscle cells using uv cured spin coated polymers |
| url | http://dx.doi.org/10.1155/2009/593410 |
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