Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells
Androgen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility. The nonclassical androgen signaling pathway is proposed to be mediated by an AR in plasma membrane in Sertoli cells. Our previous studies showed that testosterone induces cytoplasmic AR transloca...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2018/4537214 |
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| _version_ | 1832549086873518080 |
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| author | Qiong Deng Jianwen Zhang Zhu Wang Shengping Zhang Fan Zhi Hui Liang |
| author_facet | Qiong Deng Jianwen Zhang Zhu Wang Shengping Zhang Fan Zhi Hui Liang |
| author_sort | Qiong Deng |
| collection | DOAJ |
| description | Androgen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility. The nonclassical androgen signaling pathway is proposed to be mediated by an AR in plasma membrane in Sertoli cells. Our previous studies showed that testosterone induces cytoplasmic AR translocation to plasma membrane by binding with caveolin-1. This study was conducted to the underlying molecular mechanism mediating AR trafficking. Data from mass spectrometry using membrane coimmunoprecipitation sample by anti-AR antibody indicated VAPA is a candidate protein. Knockdown of VAPA by shRNA decreased the amount of AR localized to membrane and nuclear fraction and prevented AR trafficking after being exposed to testosterone. Further studies indicated AR trafficking in Sertoli cells might be mediated by VAPA via association with vesicle transport protein OSBP. This study can enrich the mechanism of the androgen actions and will be helpful for further clarifying the nonclassical signaling pathway of androgens in Sertoli cells. |
| format | Article |
| id | doaj-art-35802b92a5444c82ac1aa9b819e8d40c |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-35802b92a5444c82ac1aa9b819e8d40c2025-02-03T06:12:12ZengWileyInternational Journal of Endocrinology1687-83371687-83452018-01-01201810.1155/2018/45372144537214Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli CellsQiong Deng0Jianwen Zhang1Zhu Wang2Shengping Zhang3Fan Zhi4Hui Liang5Department of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaDepartment of Urology, The People’s Hospital of Longhua, Shenzhen, ChinaAndrogen and its receptor (AR) play an important role in maintaining spermatogenesis and male fertility. The nonclassical androgen signaling pathway is proposed to be mediated by an AR in plasma membrane in Sertoli cells. Our previous studies showed that testosterone induces cytoplasmic AR translocation to plasma membrane by binding with caveolin-1. This study was conducted to the underlying molecular mechanism mediating AR trafficking. Data from mass spectrometry using membrane coimmunoprecipitation sample by anti-AR antibody indicated VAPA is a candidate protein. Knockdown of VAPA by shRNA decreased the amount of AR localized to membrane and nuclear fraction and prevented AR trafficking after being exposed to testosterone. Further studies indicated AR trafficking in Sertoli cells might be mediated by VAPA via association with vesicle transport protein OSBP. This study can enrich the mechanism of the androgen actions and will be helpful for further clarifying the nonclassical signaling pathway of androgens in Sertoli cells.http://dx.doi.org/10.1155/2018/4537214 |
| spellingShingle | Qiong Deng Jianwen Zhang Zhu Wang Shengping Zhang Fan Zhi Hui Liang Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells International Journal of Endocrinology |
| title | Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells |
| title_full | Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells |
| title_fullStr | Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells |
| title_full_unstemmed | Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells |
| title_short | Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells |
| title_sort | vesicle associated membrane protein associated protein a is involved in androgen receptor trafficking in mouse sertoli cells |
| url | http://dx.doi.org/10.1155/2018/4537214 |
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