The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection
The application of biological therapy and glucocorticoids in Auto-immune diseases (AID) patients will cause immunocompromised host (ICH) prone to infection. And monocytes play a key role in both innate and adaptive immune responses. We aimed to investigate the changes of circulating monocyte subsets...
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| Format: | Article |
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SAGE Publishing
2025-01-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/17534259251316152 |
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| author | Danhong Zhou Yujia Jin Yifan Jin Yu Shen Qiuxia Qu Cheng Chen |
| author_facet | Danhong Zhou Yujia Jin Yifan Jin Yu Shen Qiuxia Qu Cheng Chen |
| author_sort | Danhong Zhou |
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| description | The application of biological therapy and glucocorticoids in Auto-immune diseases (AID) patients will cause immunocompromised host (ICH) prone to infection. And monocytes play a key role in both innate and adaptive immune responses. We aimed to investigate the changes of circulating monocyte subsets in AID or AID-ICH patients with pulmonary infection. The subgroups and PD-L1 expression of monocytes were measured by flow cytometry in healthy individuals (HC), new-onset AID patients (AID cohort) and AID-ICH patients with pulmonary opportunistic infection (AID-ICH cohort). Flow cytometry analysis was used to determine the distribution of monocyte subsets, including classical monocytes (CL, CD14 ++ CD16 − ), intermediate monocytes (ITM, CD14 ++ CD16 + ) and non-classical monocytes (NC, CD14 +/− CD16 ++ ), as well as the dynamic change of PD-L1 + cluster among monocyte subsets. Among total monocyte, AID-ICH displayed decreased CL subset along with increased NC subset compared to HCs and AID. Regarding PD-L1 + monocytes, although CL subset constituted the majority of that in HCs, AIDs and AID-ICHs, imbalance of NC/CL within PD-L1 + monocytes was only noticed in AID-ICHs ( P < 0.05). Furthermore, when AID subjects were developed into immunocompromised status (ICH), PD-L1 + cluster in CL was minorly decreased ( P > 0.05). Clinically, the lower ratio of PD-L1 + cluster among CL subset ( P < 0.05) and the less differentiated CL in PD-L1 + monocytes ( P < 0.05) was more likely to leaded to disease progression. The imbalance of circulating NC/CL subset was remarkable in immunocompromised host with pulmonary opportunistic infection, especially involvement of PD-L1 + cluster, which served as a potential biomarker in clinical practice. |
| format | Article |
| id | doaj-art-357e4fe2a92946399aff27b4707753f6 |
| institution | Kabale University |
| issn | 1753-4267 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj-art-357e4fe2a92946399aff27b4707753f62025-01-23T13:03:32ZengSAGE PublishingInnate Immunity1753-42672025-01-013110.1177/17534259251316152The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infectionDanhong Zhou0Yujia Jin1Yifan Jin2Yu Shen3Qiuxia Qu4Cheng Chen5 Department of Respiratory and Critical Medicine, , Suzhou, China Department of Respiratory and Critical Medicine, , Suzhou, China Department of Respiratory and Critical Medicine, , Suzhou, China Jiangsu Key Laboratory of Clinical Immunology, , Suzhou, China Jiangsu Key Laboratory of Clinical Immunology, , Suzhou, China Department of Respiratory and Critical Medicine, , Suzhou, ChinaThe application of biological therapy and glucocorticoids in Auto-immune diseases (AID) patients will cause immunocompromised host (ICH) prone to infection. And monocytes play a key role in both innate and adaptive immune responses. We aimed to investigate the changes of circulating monocyte subsets in AID or AID-ICH patients with pulmonary infection. The subgroups and PD-L1 expression of monocytes were measured by flow cytometry in healthy individuals (HC), new-onset AID patients (AID cohort) and AID-ICH patients with pulmonary opportunistic infection (AID-ICH cohort). Flow cytometry analysis was used to determine the distribution of monocyte subsets, including classical monocytes (CL, CD14 ++ CD16 − ), intermediate monocytes (ITM, CD14 ++ CD16 + ) and non-classical monocytes (NC, CD14 +/− CD16 ++ ), as well as the dynamic change of PD-L1 + cluster among monocyte subsets. Among total monocyte, AID-ICH displayed decreased CL subset along with increased NC subset compared to HCs and AID. Regarding PD-L1 + monocytes, although CL subset constituted the majority of that in HCs, AIDs and AID-ICHs, imbalance of NC/CL within PD-L1 + monocytes was only noticed in AID-ICHs ( P < 0.05). Furthermore, when AID subjects were developed into immunocompromised status (ICH), PD-L1 + cluster in CL was minorly decreased ( P > 0.05). Clinically, the lower ratio of PD-L1 + cluster among CL subset ( P < 0.05) and the less differentiated CL in PD-L1 + monocytes ( P < 0.05) was more likely to leaded to disease progression. The imbalance of circulating NC/CL subset was remarkable in immunocompromised host with pulmonary opportunistic infection, especially involvement of PD-L1 + cluster, which served as a potential biomarker in clinical practice.https://doi.org/10.1177/17534259251316152 |
| spellingShingle | Danhong Zhou Yujia Jin Yifan Jin Yu Shen Qiuxia Qu Cheng Chen The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection Innate Immunity |
| title | The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection |
| title_full | The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection |
| title_fullStr | The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection |
| title_full_unstemmed | The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection |
| title_short | The imbalance of circulating PD-L1-expressing non-classical/ classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection |
| title_sort | imbalance of circulating pd l1 expressing non classical classical monocytes is involved in immunocompromised host related pulmonary opportunistic infection |
| url | https://doi.org/10.1177/17534259251316152 |
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