Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis
Background and Aim. The best staining to evaluate liver fibrosis in liver hepatitis is still a debated topic. This study aimed to compare Masson’s trichrome (MT), Sirius Red (SR), and orcein stainings in evaluating liver fibrosis in chronic HCV hepatitis (CHC) with semiquantitative and quantitative...
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Wiley
2015-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2015/431750 |
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| author | Daniela Cabibi Fabrizio Bronte Rossana Porcasi Sabrina Ingrao Antonino Giulio Giannone Marcello Maida Maria Grazia Bavetta Salvatore Petta Vito Di Marco Vincenza Calvaruso |
| author_facet | Daniela Cabibi Fabrizio Bronte Rossana Porcasi Sabrina Ingrao Antonino Giulio Giannone Marcello Maida Maria Grazia Bavetta Salvatore Petta Vito Di Marco Vincenza Calvaruso |
| author_sort | Daniela Cabibi |
| collection | DOAJ |
| description | Background and Aim. The best staining to evaluate liver fibrosis in liver hepatitis is still a debated topic. This study aimed to compare Masson’s trichrome (MT), Sirius Red (SR), and orcein stainings in evaluating liver fibrosis in chronic HCV hepatitis (CHC) with semiquantitative and quantitative methods (Collagen Proportionate Area (CPA) by Digital Image Analysis (DIA)) and correlate them with transient elastography (TE). Methods. Liver stiffness evaluation of 111 consecutive patients with CHC was performed by TE. Semiquantitative staging by Metavir score system and CPA by DIA were assessed on liver biopsy stained with MT, SR, and orcein. Results. MT, SR, and orcein staining showed concordant results in 89.6% of cases in staging CHC, without significant difference in both semiquantitative and quantitative evaluations of fibrosis. TE values were concordant with orcein levels in 86.5% of the cases and with MT/RS in 77.5% (P<0.001). No significant correlation between the grade of necroinflammatory activity and TE values was found. Conclusion. In CHC, SR/MT and orcein stainings are almost concordant and when discordant, orcein staining is better related to TE values than MT/RS. This suggests that elastic fibers play a more important role than reticular or collagenous ones in determining stiffness values in CHC. |
| format | Article |
| id | doaj-art-3579b650846043f0bc819ff90fcb0372 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-3579b650846043f0bc819ff90fcb03722025-02-03T01:06:17ZengWileyAnalytical Cellular Pathology2210-71772210-71852015-01-01201510.1155/2015/431750431750Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic HepatitisDaniela Cabibi0Fabrizio Bronte1Rossana Porcasi2Sabrina Ingrao3Antonino Giulio Giannone4Marcello Maida5Maria Grazia Bavetta6Salvatore Petta7Vito Di Marco8Vincenza Calvaruso9Department of Sciences for the Promotion of Health, Section of Pathology, University of Palermo, Palermo, ItalyGastroenterology and Hepatology Unit, Di.Bi.M.I.S., University of Palermo, Palermo, ItalyDepartment of Sciences for the Promotion of Health, Section of Pathology, University of Palermo, Palermo, ItalyDepartment of Sciences for the Promotion of Health, Section of Pathology, University of Palermo, Palermo, ItalyDepartment of Sciences for the Promotion of Health, Section of Pathology, University of Palermo, Palermo, ItalyGastroenterology and Hepatology Unit, Di.Bi.M.I.S., University of Palermo, Palermo, ItalyGastroenterology and Hepatology Unit, Di.Bi.M.I.S., University of Palermo, Palermo, ItalyGastroenterology and Hepatology Unit, Di.Bi.M.I.S., University of Palermo, Palermo, ItalyGastroenterology and Hepatology Unit, Di.Bi.M.I.S., University of Palermo, Palermo, ItalyGastroenterology and Hepatology Unit, Di.Bi.M.I.S., University of Palermo, Palermo, ItalyBackground and Aim. The best staining to evaluate liver fibrosis in liver hepatitis is still a debated topic. This study aimed to compare Masson’s trichrome (MT), Sirius Red (SR), and orcein stainings in evaluating liver fibrosis in chronic HCV hepatitis (CHC) with semiquantitative and quantitative methods (Collagen Proportionate Area (CPA) by Digital Image Analysis (DIA)) and correlate them with transient elastography (TE). Methods. Liver stiffness evaluation of 111 consecutive patients with CHC was performed by TE. Semiquantitative staging by Metavir score system and CPA by DIA were assessed on liver biopsy stained with MT, SR, and orcein. Results. MT, SR, and orcein staining showed concordant results in 89.6% of cases in staging CHC, without significant difference in both semiquantitative and quantitative evaluations of fibrosis. TE values were concordant with orcein levels in 86.5% of the cases and with MT/RS in 77.5% (P<0.001). No significant correlation between the grade of necroinflammatory activity and TE values was found. Conclusion. In CHC, SR/MT and orcein stainings are almost concordant and when discordant, orcein staining is better related to TE values than MT/RS. This suggests that elastic fibers play a more important role than reticular or collagenous ones in determining stiffness values in CHC.http://dx.doi.org/10.1155/2015/431750 |
| spellingShingle | Daniela Cabibi Fabrizio Bronte Rossana Porcasi Sabrina Ingrao Antonino Giulio Giannone Marcello Maida Maria Grazia Bavetta Salvatore Petta Vito Di Marco Vincenza Calvaruso Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis Analytical Cellular Pathology |
| title | Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis |
| title_full | Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis |
| title_fullStr | Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis |
| title_full_unstemmed | Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis |
| title_short | Comparison of Histochemical Stainings in Evaluation of Liver Fibrosis and Correlation with Transient Elastography in Chronic Hepatitis |
| title_sort | comparison of histochemical stainings in evaluation of liver fibrosis and correlation with transient elastography in chronic hepatitis |
| url | http://dx.doi.org/10.1155/2015/431750 |
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