Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery system
Abstract This study investigates a nanoparticle-based doxycycline (DOX) delivery system targeting cervical cancer cells via the CD44 receptor. Molecular docking revealed a strong binding affinity between hyaluronic acid (HA) and CD44 (binding energy: -7.2 kJ/mol). Characterization of the HA-Chitosan...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84203-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594751482757120 |
|---|---|
| author | Sadia Anjum Ayesha Akhtar Saleh M. Aldaqal Maisa S. Abduh Hammad Ahmad Riaz Mustafa Faiza Naseer Maryam Sadia Tahir Ahmad |
| author_facet | Sadia Anjum Ayesha Akhtar Saleh M. Aldaqal Maisa S. Abduh Hammad Ahmad Riaz Mustafa Faiza Naseer Maryam Sadia Tahir Ahmad |
| author_sort | Sadia Anjum |
| collection | DOAJ |
| description | Abstract This study investigates a nanoparticle-based doxycycline (DOX) delivery system targeting cervical cancer cells via the CD44 receptor. Molecular docking revealed a strong binding affinity between hyaluronic acid (HA) and CD44 (binding energy: -7.2 kJ/mol). Characterization of the HA-Chitosan nanoparticles showed a particle size of 284.6 nm, a zeta potential of 16.9 mV, and a polydispersity index of 0.314, with SEM confirming smooth surface morphology. The encapsulation efficiency of DOX-loaded nanoparticles was 89.32%, exhibiting a sustained release profile, with 67.45% released over 72 h in acidic conditions (pH 5.5). Cytotoxicity assays demonstrated a significant reduction in HeLa cell viability to 22% at 72 h, compared to 67% in normal HEK cells. Stability tests confirmed the maintenance of nanoparticle integrity and a consistent drug release profile over three months. Cell migration was reduced by 45%, and RT-PCR analysis revealed a 53% downregulation of TNF-α expression, suggesting effective targeting of inflammatory pathways. These results underscore the potential of HA-Chitosan-based DOX nanoparticles in improving cervical cancer treatment through enhanced targeted delivery and inhibition of tumor-promoting mechanisms. |
| format | Article |
| id | doaj-art-357076a403524a4f84efdb75bbd7dad0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-357076a403524a4f84efdb75bbd7dad02025-01-19T12:21:48ZengNature PortfolioScientific Reports2045-23222025-01-0115112010.1038/s41598-024-84203-8Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery systemSadia Anjum0Ayesha Akhtar1Saleh M. Aldaqal2Maisa S. Abduh3Hammad Ahmad4Riaz Mustafa5Faiza Naseer6Maryam Sadia7Tahir Ahmad8Department of Biology, University of HailIndustrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and TechnologyImmune Responses in Different Diseases Research Group, Department of Surgery, Faculty of Medicine, King Abdulaziz UniversityImmune Responses in Different Diseases Research Group, Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdul-Aziz UniversityDepartment of Pharmacy, Bashir Institute of Health SciencesDepartment of Pathology, University of Agriculture Faisalabad, Sub campus Toba Tek SinghDepartment of Biosciences, Shifa Tameer e Millat UniversityIndustrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and TechnologyIndustrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and TechnologyAbstract This study investigates a nanoparticle-based doxycycline (DOX) delivery system targeting cervical cancer cells via the CD44 receptor. Molecular docking revealed a strong binding affinity between hyaluronic acid (HA) and CD44 (binding energy: -7.2 kJ/mol). Characterization of the HA-Chitosan nanoparticles showed a particle size of 284.6 nm, a zeta potential of 16.9 mV, and a polydispersity index of 0.314, with SEM confirming smooth surface morphology. The encapsulation efficiency of DOX-loaded nanoparticles was 89.32%, exhibiting a sustained release profile, with 67.45% released over 72 h in acidic conditions (pH 5.5). Cytotoxicity assays demonstrated a significant reduction in HeLa cell viability to 22% at 72 h, compared to 67% in normal HEK cells. Stability tests confirmed the maintenance of nanoparticle integrity and a consistent drug release profile over three months. Cell migration was reduced by 45%, and RT-PCR analysis revealed a 53% downregulation of TNF-α expression, suggesting effective targeting of inflammatory pathways. These results underscore the potential of HA-Chitosan-based DOX nanoparticles in improving cervical cancer treatment through enhanced targeted delivery and inhibition of tumor-promoting mechanisms.https://doi.org/10.1038/s41598-024-84203-8DoxycyclineCervical CancerNovel Drug Delivery SystemSustained releaseTargeted therapyImmunotherapy |
| spellingShingle | Sadia Anjum Ayesha Akhtar Saleh M. Aldaqal Maisa S. Abduh Hammad Ahmad Riaz Mustafa Faiza Naseer Maryam Sadia Tahir Ahmad Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery system Scientific Reports Doxycycline Cervical Cancer Novel Drug Delivery System Sustained release Targeted therapy Immunotherapy |
| title | Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery system |
| title_full | Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery system |
| title_fullStr | Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery system |
| title_full_unstemmed | Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery system |
| title_short | Enhanced targeted treatment of cervical cancer using nanoparticle-based doxycycline delivery system |
| title_sort | enhanced targeted treatment of cervical cancer using nanoparticle based doxycycline delivery system |
| topic | Doxycycline Cervical Cancer Novel Drug Delivery System Sustained release Targeted therapy Immunotherapy |
| url | https://doi.org/10.1038/s41598-024-84203-8 |
| work_keys_str_mv | AT sadiaanjum enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT ayeshaakhtar enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT salehmaldaqal enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT maisasabduh enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT hammadahmad enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT riazmustafa enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT faizanaseer enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT maryamsadia enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem AT tahirahmad enhancedtargetedtreatmentofcervicalcancerusingnanoparticlebaseddoxycyclinedeliverysystem |