Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period

Daphnia with a density of 10,000 ind./l medium were enriched with either 0.1, 0.5 or 1 g l-ascorbic acid ethyl cellulose (VC) per l culture medium. In this triplicate experiment, Daphnia were enriched with VC in a 12 litre aquaria for 0, 3, 6 or 9 h. Results showed that the maximum VC content in Da...

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Main Authors: D. Jusadi, I. Meylani, N.B.P. Utomo
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2008-01-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/4033
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author D. Jusadi
I. Meylani
N.B.P. Utomo
author_facet D. Jusadi
I. Meylani
N.B.P. Utomo
author_sort D. Jusadi
collection DOAJ
description Daphnia with a density of 10,000 ind./l medium were enriched with either 0.1, 0.5 or 1 g l-ascorbic acid ethyl cellulose (VC) per l culture medium. In this triplicate experiment, Daphnia were enriched with VC in a 12 litre aquaria for 0, 3, 6 or 9 h. Results showed that the maximum VC content in Daphnia enriched with 0.1, 0.5 and 1 g VC/l medium was 347, 471 and 482 mg VC/g Daphnia (dry basis), respectively. This VC content was obtained after 5 h, 5 h 12 min and 5 h 18 min of enrichment period.  During 9 h enrichment period, the survival rate of Daphnia enriched with 0.1, 0.5 and 1 g VC/l medium was decrease linearly from 100% to 55.6%, 50.1% and 47.9%, respectively. Keywords: Daphnia, vitamin C, enrichment   ABSTRAK Dalam penelitian ini,  Daphnia dengan padat tebar 10.000 ekor/l diperkaya dengan vitamin C (VC) pada tiga dosis yang berbeda, yaitu dosis 0,1, 0,5 dan 1 g VC/l media untuk masing-masing penelitian 1, 2 dan 3.  Daphnia diperkaya dalam wadah volume 12 liter selama 0, 3, 6 dan 9 jam.  Masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil analisa respon menunjukkan bahwa waktu pengkayaan yang optimum untuk masing-masing dosis pengkayaan 0,1, 0,5 dan 1 g VC/l media secara berturut-turut adalah 5 jam, 5 jam 12 menit dan 5 jam 18 menit dengan kadar VC masing-masing sebesar 347, 471 dan 482 mg VC/g Daphnia.  Tingkat kelangsungan hidup Daphnia sp. menunjukkan terjadinya penurunan secara linier dengan semakin bertambahnya waktu pengkayaan.  Tingkat kelangsungan hidup Daphnia sp. setelah 9 jam masa pengkayaan ialah sebesar 55,63%, 50,12% dan 47,92% untuk dosis 0,1, 0,5 dan 1 g VC/l media.  Kata kunci: Daphnia, vitamin C, pengkayaan
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2354-6700
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spelling doaj-art-356a48aca6764699b17dc98f6ae04e3d2025-01-24T18:02:43ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002008-01-017110.19027/jai.7.11-173641Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment PeriodD. Jusadi0I. Meylani1N.B.P. Utomo2Bogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of Aquaculture Daphnia with a density of 10,000 ind./l medium were enriched with either 0.1, 0.5 or 1 g l-ascorbic acid ethyl cellulose (VC) per l culture medium. In this triplicate experiment, Daphnia were enriched with VC in a 12 litre aquaria for 0, 3, 6 or 9 h. Results showed that the maximum VC content in Daphnia enriched with 0.1, 0.5 and 1 g VC/l medium was 347, 471 and 482 mg VC/g Daphnia (dry basis), respectively. This VC content was obtained after 5 h, 5 h 12 min and 5 h 18 min of enrichment period.  During 9 h enrichment period, the survival rate of Daphnia enriched with 0.1, 0.5 and 1 g VC/l medium was decrease linearly from 100% to 55.6%, 50.1% and 47.9%, respectively. Keywords: Daphnia, vitamin C, enrichment   ABSTRAK Dalam penelitian ini,  Daphnia dengan padat tebar 10.000 ekor/l diperkaya dengan vitamin C (VC) pada tiga dosis yang berbeda, yaitu dosis 0,1, 0,5 dan 1 g VC/l media untuk masing-masing penelitian 1, 2 dan 3.  Daphnia diperkaya dalam wadah volume 12 liter selama 0, 3, 6 dan 9 jam.  Masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil analisa respon menunjukkan bahwa waktu pengkayaan yang optimum untuk masing-masing dosis pengkayaan 0,1, 0,5 dan 1 g VC/l media secara berturut-turut adalah 5 jam, 5 jam 12 menit dan 5 jam 18 menit dengan kadar VC masing-masing sebesar 347, 471 dan 482 mg VC/g Daphnia.  Tingkat kelangsungan hidup Daphnia sp. menunjukkan terjadinya penurunan secara linier dengan semakin bertambahnya waktu pengkayaan.  Tingkat kelangsungan hidup Daphnia sp. setelah 9 jam masa pengkayaan ialah sebesar 55,63%, 50,12% dan 47,92% untuk dosis 0,1, 0,5 dan 1 g VC/l media.  Kata kunci: Daphnia, vitamin C, pengkayaan https://journal.ipb.ac.id/index.php/jai/article/view/4033
spellingShingle D. Jusadi
I. Meylani
N.B.P. Utomo
Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
Jurnal Akuakultur Indonesia
title Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
title_full Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
title_fullStr Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
title_full_unstemmed Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
title_short Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
title_sort vitamin c content in daphnia sp enriched with l ascorbic acid ethyl cellulose at different enrichment period
url https://journal.ipb.ac.id/index.php/jai/article/view/4033
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AT imeylani vitaminccontentindaphniaspenrichedwithlascorbicacidethylcelluloseatdifferentenrichmentperiod
AT nbputomo vitaminccontentindaphniaspenrichedwithlascorbicacidethylcelluloseatdifferentenrichmentperiod