Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production
Abstract Background Phaeodactylum tricornutum is a versatile marine microalga renowned for its high-value metabolite production, including omega-3 fatty acids and fucoxanthin, with emerging potential for integrated biorefinery approaches that encompass biofuel and bioproduct generation. Therefore, i...
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BMC
2025-01-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
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| Online Access: | https://doi.org/10.1186/s13068-024-02602-5 |
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| author | Mostafa E. Elshobary Walaa A. Abo-Shanab Stephan S. W. Ende Mohammed Alquraishi Rania A. El-Shenody |
| author_facet | Mostafa E. Elshobary Walaa A. Abo-Shanab Stephan S. W. Ende Mohammed Alquraishi Rania A. El-Shenody |
| author_sort | Mostafa E. Elshobary |
| collection | DOAJ |
| description | Abstract Background Phaeodactylum tricornutum is a versatile marine microalga renowned for its high-value metabolite production, including omega-3 fatty acids and fucoxanthin, with emerging potential for integrated biorefinery approaches that encompass biofuel and bioproduct generation. Therefore, in this study we aimed to optimize the cultivation conditions for boosting biomass, lipid, and fucoxanthin production in P. tricornutum, focusing on the impacts of different nutrient ratios (nitrogen, phosphorus, silicate), glycerol supplementation, and light regimes. Results Optimized medium (− 50%N%, + 50% P, Zero-Si, 2 g glycerol) under low-intensity blue light (100 μmol m⁻2 s⁻1) improved biomass to 1.6 g L⁻1, with lipid productivity reaching 539.25 mg g⁻1, while fucoxanthin increased to 20.44 mg g−1. Total saturated fatty acid (ΣSFA) content in the optimized culture increased approximately 2.4-fold compared to the control F/2 medium. This change in fatty acid composition led to improved biodiesel properties, including a higher cetane number (59.18 vs. 56.04) and lower iodine value (53.96 vs 88.99 g I2/100 g oil). The optimized conditions also altered the biodiesel characteristics, such as kinematic viscosity, cloud point, and higher heating value. Conclusion Our optimization approach reveals the significant potential of P. tricornutum as a versatile microbial platform for biomass, lipid, and fucoxanthin production. The tailored cultivation strategy successfully enhanced biomass and lipid accumulation, with notable improvements in biodiesel properties through strategic nutrient and light regime manipulation. These findings demonstrate the critical role of precise cultivation conditions in optimizing microalgal metabolic performance for biotechnological applications. |
| format | Article |
| id | doaj-art-3561a16626844f28bfe0e8e5ecab605b |
| institution | Kabale University |
| issn | 2731-3654 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj-art-3561a16626844f28bfe0e8e5ecab605b2025-01-19T12:13:45ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542025-01-0118112210.1186/s13068-024-02602-5Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin productionMostafa E. Elshobary0Walaa A. Abo-Shanab1Stephan S. W. Ende2Mohammed Alquraishi3Rania A. El-Shenody4Botany and Microbiology Department, Faculty of Science, Tanta UniversityBotany and Microbiology Department, Faculty of Science, Tanta UniversityAquaculture Research, Alfred Wegener Institute (AWI) – Helmholtz Centre for Polar and Marine ResearchDepartment of Community Health Sciences, College of Applied Medical Sciences, King Saud UniversityBotany and Microbiology Department, Faculty of Science, Tanta UniversityAbstract Background Phaeodactylum tricornutum is a versatile marine microalga renowned for its high-value metabolite production, including omega-3 fatty acids and fucoxanthin, with emerging potential for integrated biorefinery approaches that encompass biofuel and bioproduct generation. Therefore, in this study we aimed to optimize the cultivation conditions for boosting biomass, lipid, and fucoxanthin production in P. tricornutum, focusing on the impacts of different nutrient ratios (nitrogen, phosphorus, silicate), glycerol supplementation, and light regimes. Results Optimized medium (− 50%N%, + 50% P, Zero-Si, 2 g glycerol) under low-intensity blue light (100 μmol m⁻2 s⁻1) improved biomass to 1.6 g L⁻1, with lipid productivity reaching 539.25 mg g⁻1, while fucoxanthin increased to 20.44 mg g−1. Total saturated fatty acid (ΣSFA) content in the optimized culture increased approximately 2.4-fold compared to the control F/2 medium. This change in fatty acid composition led to improved biodiesel properties, including a higher cetane number (59.18 vs. 56.04) and lower iodine value (53.96 vs 88.99 g I2/100 g oil). The optimized conditions also altered the biodiesel characteristics, such as kinematic viscosity, cloud point, and higher heating value. Conclusion Our optimization approach reveals the significant potential of P. tricornutum as a versatile microbial platform for biomass, lipid, and fucoxanthin production. The tailored cultivation strategy successfully enhanced biomass and lipid accumulation, with notable improvements in biodiesel properties through strategic nutrient and light regime manipulation. These findings demonstrate the critical role of precise cultivation conditions in optimizing microalgal metabolic performance for biotechnological applications.https://doi.org/10.1186/s13068-024-02602-5DiatomFucoxanthinBiodiesel propertiesLipid optimizationFatty acid profileMixotrophic cultivation |
| spellingShingle | Mostafa E. Elshobary Walaa A. Abo-Shanab Stephan S. W. Ende Mohammed Alquraishi Rania A. El-Shenody Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production Biotechnology for Biofuels and Bioproducts Diatom Fucoxanthin Biodiesel properties Lipid optimization Fatty acid profile Mixotrophic cultivation |
| title | Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production |
| title_full | Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production |
| title_fullStr | Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production |
| title_full_unstemmed | Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production |
| title_short | Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production |
| title_sort | optimizing phaeodactylum tricornutum cultivation integrated strategies for enhancing biomass lipid and fucoxanthin production |
| topic | Diatom Fucoxanthin Biodiesel properties Lipid optimization Fatty acid profile Mixotrophic cultivation |
| url | https://doi.org/10.1186/s13068-024-02602-5 |
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