Effects of age and diet on triglyceride metabolism in mice
Both age and diet can contribute to alterations in triglyceride metabolism and subsequent metabolic disease. In humans, plasma triglyceride levels increase with age. Diets high in saturated fats can increase triglyceride levels while diets high in omega-3 fatty acids decrease triglyceride levels. He...
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Elsevier
2025-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227524002116 |
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| author | Kathryn M. Spitler Shwetha K. Shetty Brandon S.J. Davies |
| author_facet | Kathryn M. Spitler Shwetha K. Shetty Brandon S.J. Davies |
| author_sort | Kathryn M. Spitler |
| collection | DOAJ |
| description | Both age and diet can contribute to alterations in triglyceride metabolism and subsequent metabolic disease. In humans, plasma triglyceride levels increase with age. Diets high in saturated fats can increase triglyceride levels while diets high in omega-3 fatty acids decrease triglyceride levels. Here we asked how age and long-term diet altered triglyceride metabolism in mice. We fed male and female C57Bl/6 mice a low-fat diet, a western diet (WD), or a diet high in polyunsaturated and omega-3 fatty acids (n3D) for up to 2 years. We measured survival, body composition, plasma triglyceride levels, chylomicron clearance, and oral fat, glucose, and insulin tolerance. Triglyceride levels in mice did not increase with age, regardless of diet. Oral fat tolerance increased with age, while chylomicron clearance remained unchanged. Decreased survival was observed in WD-fed mice. Interestingly, n3D-fed mice gained more lean mass and had lower insulin levels than WD-fed or LFD-fed mice. Moreover, triglyceride uptake into the hearts of n3D-fed mice was strikingly higher than in other groups. Our data indicate that in C57Bl/6 mice, age-induced changes in triglyceride metabolism differ from those observed in humans. Mice, like humans, appeared to have decreased fat absorption with age, but in mice plasma triglyceride clearance did not decrease with age, resulting in lower plasma triglyceride levels and improved fat tolerance with age. Although a chronic diet high in omega-3 fatty acids increased insulin sensitivity and triglyceride uptake specifically into the heart, how these observations are connected is unclear. |
| format | Article |
| id | doaj-art-355facf507464cdeb6dc24223baecca7 |
| institution | Kabale University |
| issn | 0022-2275 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Journal of Lipid Research |
| spelling | doaj-art-355facf507464cdeb6dc24223baecca72025-01-30T05:12:37ZengElsevierJournal of Lipid Research0022-22752025-01-01661100706Effects of age and diet on triglyceride metabolism in miceKathryn M. Spitler0Shwetha K. Shetty1Brandon S.J. Davies2Department of Biochemistry and Molecular Biology, Fraternal Order of Eagles Diabetes Research Center, and Obesity Research and Education Initiative, University of Iowa, Iowa City, IADepartment of Biochemistry and Molecular Biology, Fraternal Order of Eagles Diabetes Research Center, and Obesity Research and Education Initiative, University of Iowa, Iowa City, IAFor correspondence: Brandon S. J. Davies; Department of Biochemistry and Molecular Biology, Fraternal Order of Eagles Diabetes Research Center, and Obesity Research and Education Initiative, University of Iowa, Iowa City, IABoth age and diet can contribute to alterations in triglyceride metabolism and subsequent metabolic disease. In humans, plasma triglyceride levels increase with age. Diets high in saturated fats can increase triglyceride levels while diets high in omega-3 fatty acids decrease triglyceride levels. Here we asked how age and long-term diet altered triglyceride metabolism in mice. We fed male and female C57Bl/6 mice a low-fat diet, a western diet (WD), or a diet high in polyunsaturated and omega-3 fatty acids (n3D) for up to 2 years. We measured survival, body composition, plasma triglyceride levels, chylomicron clearance, and oral fat, glucose, and insulin tolerance. Triglyceride levels in mice did not increase with age, regardless of diet. Oral fat tolerance increased with age, while chylomicron clearance remained unchanged. Decreased survival was observed in WD-fed mice. Interestingly, n3D-fed mice gained more lean mass and had lower insulin levels than WD-fed or LFD-fed mice. Moreover, triglyceride uptake into the hearts of n3D-fed mice was strikingly higher than in other groups. Our data indicate that in C57Bl/6 mice, age-induced changes in triglyceride metabolism differ from those observed in humans. Mice, like humans, appeared to have decreased fat absorption with age, but in mice plasma triglyceride clearance did not decrease with age, resulting in lower plasma triglyceride levels and improved fat tolerance with age. Although a chronic diet high in omega-3 fatty acids increased insulin sensitivity and triglyceride uptake specifically into the heart, how these observations are connected is unclear.http://www.sciencedirect.com/science/article/pii/S0022227524002116lipoprotein metabolismomega-3 fatty acidsagingtriglycerides |
| spellingShingle | Kathryn M. Spitler Shwetha K. Shetty Brandon S.J. Davies Effects of age and diet on triglyceride metabolism in mice Journal of Lipid Research lipoprotein metabolism omega-3 fatty acids aging triglycerides |
| title | Effects of age and diet on triglyceride metabolism in mice |
| title_full | Effects of age and diet on triglyceride metabolism in mice |
| title_fullStr | Effects of age and diet on triglyceride metabolism in mice |
| title_full_unstemmed | Effects of age and diet on triglyceride metabolism in mice |
| title_short | Effects of age and diet on triglyceride metabolism in mice |
| title_sort | effects of age and diet on triglyceride metabolism in mice |
| topic | lipoprotein metabolism omega-3 fatty acids aging triglycerides |
| url | http://www.sciencedirect.com/science/article/pii/S0022227524002116 |
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