Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice
The aim of this study was to investigate the antioxidant effects of flavonoid extract of lotus leaf (FELL) in vitro and in mice with gastric injury and elucidate the inhibitory effect of FELL on alcohol-induced gastric injury by antioxidant capacity. Gastric juice volume and acid value were measured...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2020/1206247 |
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| author | Yi Chen Qin Li Zhiping Kuang Xin Zhao Ruokun Yi Xiaowei He Guangjing Feng |
| author_facet | Yi Chen Qin Li Zhiping Kuang Xin Zhao Ruokun Yi Xiaowei He Guangjing Feng |
| author_sort | Yi Chen |
| collection | DOAJ |
| description | The aim of this study was to investigate the antioxidant effects of flavonoid extract of lotus leaf (FELL) in vitro and in mice with gastric injury and elucidate the inhibitory effect of FELL on alcohol-induced gastric injury by antioxidant capacity. Gastric juice volume and acid value were measured in the established alcohol-induced acute gastric injury model in mice. Hematoxylin and eosin staining demonstrated the morphological changes in the gastric mucosa. FELL improved the alcohol-induced gastric lesions. Serological testing results showed that FELL significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and nitric oxide (NO) and significantly reduced the levels of MDA and MPO in mice with gastric injury. Besides, FELL significantly reduced the levels of interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-α), and interferon gamma (IFN-γ). The quantitative polymerase chain reaction (qPCR) and Western blot results proved that FELL upregulated the mRNA and protein expressions of SOD1, SOD2, CAT, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS) and downregulated the expression of inducible nitric oxide synthase (iNOS) in the gastric tissue of mice with gastric injury. Five compounds, including kaempferitrin, hyperoside, astragalin, phloridzin, and quercetin, were detected in FELL using high-performance liquid chromatography. Thus, FELL has an inhibitory effect on experimental alcohol-induced gastric injury because of the antioxidant capacity of the five compounds. Its effect increases as the FELL concentration increases, which is close to that of ranitidine. FELL, which is an active substance, provides a good gastroprotective effect. |
| format | Article |
| id | doaj-art-3522f01b11064ca39a0af7c1fc5dd250 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-3522f01b11064ca39a0af7c1fc5dd2502025-02-03T01:32:23ZengWileyJournal of Food Quality0146-94281745-45572020-01-01202010.1155/2020/12062471206247Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in MiceYi Chen0Qin Li1Zhiping Kuang2Xin Zhao3Ruokun Yi4Xiaowei He5Guangjing Feng6Department of General Surgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, ChinaDepartment of Ophthalmology, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, ChinaThe First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaDepartment of General Surgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, ChinaDepartment of General Surgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, ChinaThe aim of this study was to investigate the antioxidant effects of flavonoid extract of lotus leaf (FELL) in vitro and in mice with gastric injury and elucidate the inhibitory effect of FELL on alcohol-induced gastric injury by antioxidant capacity. Gastric juice volume and acid value were measured in the established alcohol-induced acute gastric injury model in mice. Hematoxylin and eosin staining demonstrated the morphological changes in the gastric mucosa. FELL improved the alcohol-induced gastric lesions. Serological testing results showed that FELL significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and nitric oxide (NO) and significantly reduced the levels of MDA and MPO in mice with gastric injury. Besides, FELL significantly reduced the levels of interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNF-α), and interferon gamma (IFN-γ). The quantitative polymerase chain reaction (qPCR) and Western blot results proved that FELL upregulated the mRNA and protein expressions of SOD1, SOD2, CAT, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS) and downregulated the expression of inducible nitric oxide synthase (iNOS) in the gastric tissue of mice with gastric injury. Five compounds, including kaempferitrin, hyperoside, astragalin, phloridzin, and quercetin, were detected in FELL using high-performance liquid chromatography. Thus, FELL has an inhibitory effect on experimental alcohol-induced gastric injury because of the antioxidant capacity of the five compounds. Its effect increases as the FELL concentration increases, which is close to that of ranitidine. FELL, which is an active substance, provides a good gastroprotective effect.http://dx.doi.org/10.1155/2020/1206247 |
| spellingShingle | Yi Chen Qin Li Zhiping Kuang Xin Zhao Ruokun Yi Xiaowei He Guangjing Feng Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice Journal of Food Quality |
| title | Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice |
| title_full | Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice |
| title_fullStr | Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice |
| title_full_unstemmed | Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice |
| title_short | Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice |
| title_sort | inhibitory effect of flavonoid extract of lotus leaf on alcohol induced gastric injury by antioxidant capacity in mice |
| url | http://dx.doi.org/10.1155/2020/1206247 |
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