The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response
Based on Toll/interleukin-1 receptor (TIR) domain structure homology, we detected a previously uncharacterized gene encoding for a TIR domain containing protein (Tcp) in the genome of Enterococcus faecalis. We assigned this gene the name tcpF (as in Tcp of E. faecalis). Screening of E. faecalis samp...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2014/918143 |
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| author | Thomas Daniel Kraemer Orlando Daniel Quintanar Haro Eugen Domann Trinad Chakraborty Svetlin Tchatalbachev |
| author_facet | Thomas Daniel Kraemer Orlando Daniel Quintanar Haro Eugen Domann Trinad Chakraborty Svetlin Tchatalbachev |
| author_sort | Thomas Daniel Kraemer |
| collection | DOAJ |
| description | Based on Toll/interleukin-1 receptor (TIR) domain structure homology, we detected a previously uncharacterized gene encoding for a TIR domain containing protein (Tcp) in the genome of Enterococcus faecalis. We assigned this gene the name tcpF (as in Tcp of E. faecalis). Screening of E. faecalis samples revealed that tcpF is more common in isolates from urinary tract infections (UTIs) than in human faecal flora. tcpF alleles showed moderate single nucleotide polymorphism (SNP) among UTI isolates. Infection of mouse RAW264.7 macrophages with a tcpF knock-out mutant led to elevated cytokine response compared to the isogenic wild type E. faecalis strain. In silico analysis predicted significant tertiary structure homology to the TIR domain of human TLR1 (TLR1-TIR). When transiently expressed in cultured eukaryotic cells, TcpF caused suppression of TLR2-dependent NF-κB activation suggesting for TcpF a role as a factor in E. faecalis that benefits colonization by modulating the host’s immune responses. |
| format | Article |
| id | doaj-art-35042355ea7b4587b39cf22a339538cd |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-35042355ea7b4587b39cf22a339538cd2025-02-03T05:49:56ZengWileyInternational Journal of Microbiology1687-918X1687-91982014-01-01201410.1155/2014/918143918143The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory ResponseThomas Daniel Kraemer0Orlando Daniel Quintanar Haro1Eugen Domann2Trinad Chakraborty3Svetlin Tchatalbachev4Institute of Medical Microbiology, Justus Liebig University, Schubertstraße 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University, Schubertstraße 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University, Schubertstraße 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University, Schubertstraße 81, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus Liebig University, Schubertstraße 81, 35392 Giessen, GermanyBased on Toll/interleukin-1 receptor (TIR) domain structure homology, we detected a previously uncharacterized gene encoding for a TIR domain containing protein (Tcp) in the genome of Enterococcus faecalis. We assigned this gene the name tcpF (as in Tcp of E. faecalis). Screening of E. faecalis samples revealed that tcpF is more common in isolates from urinary tract infections (UTIs) than in human faecal flora. tcpF alleles showed moderate single nucleotide polymorphism (SNP) among UTI isolates. Infection of mouse RAW264.7 macrophages with a tcpF knock-out mutant led to elevated cytokine response compared to the isogenic wild type E. faecalis strain. In silico analysis predicted significant tertiary structure homology to the TIR domain of human TLR1 (TLR1-TIR). When transiently expressed in cultured eukaryotic cells, TcpF caused suppression of TLR2-dependent NF-κB activation suggesting for TcpF a role as a factor in E. faecalis that benefits colonization by modulating the host’s immune responses.http://dx.doi.org/10.1155/2014/918143 |
| spellingShingle | Thomas Daniel Kraemer Orlando Daniel Quintanar Haro Eugen Domann Trinad Chakraborty Svetlin Tchatalbachev The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response International Journal of Microbiology |
| title | The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response |
| title_full | The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response |
| title_fullStr | The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response |
| title_full_unstemmed | The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response |
| title_short | The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response |
| title_sort | tir domain containing locus of enterococcus faecalis is predominant among urinary tract infection isolates and downregulates host inflammatory response |
| url | http://dx.doi.org/10.1155/2014/918143 |
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