Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents
The aims of this study were to evaluate the anti-inflammatory and analgesic effects of bufalin, a major component of “Chan-su.” We used a carrageenan-induced paw edema model to assess the anti-inflammatory activity of this compound, and Western blot analysis detected NF-κB signaling during this effe...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/171839 |
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| author | Lili Wen Yang Huang Xianbiao Xie Wan Huang Junqiang Yin Wenqian Lin Qiang Jia Weian Zeng |
| author_facet | Lili Wen Yang Huang Xianbiao Xie Wan Huang Junqiang Yin Wenqian Lin Qiang Jia Weian Zeng |
| author_sort | Lili Wen |
| collection | DOAJ |
| description | The aims of this study were to evaluate the anti-inflammatory and analgesic effects of bufalin, a major component of “Chan-su.” We used a carrageenan-induced paw edema model to assess the anti-inflammatory activity of this compound, and Western blot analysis detected NF-κB signaling during this effect. The antinociceptive activities were evaluated by acetic acid-induced writhing, formalin, and hot-plate tests; open-field test investigated effects on the central nervous system. Our data showed that bufalin (0.3 and 0.6 mg/kg, i.p.) potently decreased carrageenan-induced paw edema. Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) during these treatments. Further studies demonstrated that bufalin significantly inhibited the activation of NF-κB signaling. Bufalin also reduced acetic acid-induced writhing and the licking time in the formalin test and increased hot-plate reaction latencies. Naloxone pretreatment (2 mg/kg, i.p.) in the early phases of the formalin test and hot-plate test significantly attenuated the bufalin-induced antinociception effects, which suggests the involvement of the opioid system. A reduction in locomotion was not observed in the open-field test after bufalin administration. Taken together, bufalin treatment resulted in in vivo anti-inflammatory and analgesic effects, and bufalin may be a novel, potential drug for the treatment of inflammatory diseases. |
| format | Article |
| id | doaj-art-34c1e28f5d49464892dc1c808c7f2b8b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-34c1e28f5d49464892dc1c808c7f2b8b2025-02-03T05:58:14ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/171839171839Anti-Inflammatory and Antinociceptive Activities of Bufalin in RodentsLili Wen0Yang Huang1Xianbiao Xie2Wan Huang3Junqiang Yin4Wenqian Lin5Qiang Jia6Weian Zeng7Department of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, ChinaDepartment of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, ChinaDepartment of Orthopaedic Oncology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, ChinaDepartment of Orthopaedic Oncology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, ChinaThe Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, ChinaDepartment of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, ChinaThe aims of this study were to evaluate the anti-inflammatory and analgesic effects of bufalin, a major component of “Chan-su.” We used a carrageenan-induced paw edema model to assess the anti-inflammatory activity of this compound, and Western blot analysis detected NF-κB signaling during this effect. The antinociceptive activities were evaluated by acetic acid-induced writhing, formalin, and hot-plate tests; open-field test investigated effects on the central nervous system. Our data showed that bufalin (0.3 and 0.6 mg/kg, i.p.) potently decreased carrageenan-induced paw edema. Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) during these treatments. Further studies demonstrated that bufalin significantly inhibited the activation of NF-κB signaling. Bufalin also reduced acetic acid-induced writhing and the licking time in the formalin test and increased hot-plate reaction latencies. Naloxone pretreatment (2 mg/kg, i.p.) in the early phases of the formalin test and hot-plate test significantly attenuated the bufalin-induced antinociception effects, which suggests the involvement of the opioid system. A reduction in locomotion was not observed in the open-field test after bufalin administration. Taken together, bufalin treatment resulted in in vivo anti-inflammatory and analgesic effects, and bufalin may be a novel, potential drug for the treatment of inflammatory diseases.http://dx.doi.org/10.1155/2014/171839 |
| spellingShingle | Lili Wen Yang Huang Xianbiao Xie Wan Huang Junqiang Yin Wenqian Lin Qiang Jia Weian Zeng Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents Mediators of Inflammation |
| title | Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents |
| title_full | Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents |
| title_fullStr | Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents |
| title_full_unstemmed | Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents |
| title_short | Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents |
| title_sort | anti inflammatory and antinociceptive activities of bufalin in rodents |
| url | http://dx.doi.org/10.1155/2014/171839 |
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