Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection
Proteus mirabilis is the third most common bacterium that can cause complicated UTI, especially in catheterized patients. Urovirulence genes of P. mirabilis strains are poorly identified among UTI patients. The aims of the present study were to determine the prevalence of the uropathogenic P. mirabi...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/1231807 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553935757377536 |
|---|---|
| author | Emad I. Hussein Khalid Al-Batayneh Majed M. Masadeh Fatina W. Dahadhah Mazhar Salim Al Zoubi Alaa A. Aljabali Karem H. Alzoubi |
| author_facet | Emad I. Hussein Khalid Al-Batayneh Majed M. Masadeh Fatina W. Dahadhah Mazhar Salim Al Zoubi Alaa A. Aljabali Karem H. Alzoubi |
| author_sort | Emad I. Hussein |
| collection | DOAJ |
| description | Proteus mirabilis is the third most common bacterium that can cause complicated UTI, especially in catheterized patients. Urovirulence genes of P. mirabilis strains are poorly identified among UTI patients. The aims of the present study were to determine the prevalence of the uropathogenic P. mirabilis strains isolated from UTI patients by the detection of several P. mirabilis virulence genes and to characterize the antibiotic susceptibility profile of P. mirabilis isolates. P. mirabilis isolates were collected from urine specimens of patients suffering from UTI. Virulence genes in P. mirabilis, namely, hpmA, hpmB, rsbA, luxS, ureC1, hlyA, rpoA, atfA, atfC, mrpA, and pm1 were detected in the isolates via PCR detection method. All P. mirabilis virulence genes were detected in more than 90% of the isolates except hlyA gene, which was detected in only 23.8% of the isolates. The rate of susceptibility for ceftriaxone was 96.8%, followed by norfloxacin (82.5%), gentamicin (71.4%), ciprofloxacin (69.8%), cephalexin (52.4%), nalidixic acid (42.9%), sulfamethoxazole (39.7%), ampicillin (36.5%), and nitrofurantoin (3.2%). Significant associations (P<0.05) were detected between antimicrobial susceptibility of each of the following antibiotics and the presence virulence genes. Cephalexin antimicrobial susceptibility was significantly associated with the presence each of ureC1 and atfC. Sulfamethoxazole antimicrobial susceptibility was significantly associated with the presence atfA. Ceftriaxone antimicrobial susceptibility was significantly associated with the presence each of hpmA, ureC1, rpoA, atfC, mrpA, and pm1. Nitrofurantoin antimicrobial susceptibility was significantly associated with the presence each of hpmA, ureC1, rpoA, atfA, atfC, mrpA, and pm1. In conclusion, an association between the presence of urovirulence genes of P. mirabilis and increasing P. mirabilis resistance to antimicrobials has been demonstrated. |
| format | Article |
| id | doaj-art-34aeff60da954840be21e86669b1be47 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-34aeff60da954840be21e86669b1be472025-02-03T05:52:43ZengWileyInternational Journal of Microbiology1687-918X1687-91982020-01-01202010.1155/2020/12318071231807Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract InfectionEmad I. Hussein0Khalid Al-Batayneh1Majed M. Masadeh2Fatina W. Dahadhah3Mazhar Salim Al Zoubi4Alaa A. Aljabali5Karem H. Alzoubi6Department of Biological Sciences, Yarmouk University, Irbid 21163, JordanDepartment of Biological Sciences, Yarmouk University, Irbid 21163, JordanDepartment of Pharmaceutical Technology, Jordan University of Science and Technology, Irbid 22110, JordanDepartment of Biological Sciences, Yarmouk University, Irbid 21163, JordanDepartment of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid 21163, JordanDepartment of Pharmaceutical Sciences, Yarmouk University, Irbid 21163, JordanDepartment of Clinical Pharmacy, Jordan University of Science and Technology, Irbid 22110, JordanProteus mirabilis is the third most common bacterium that can cause complicated UTI, especially in catheterized patients. Urovirulence genes of P. mirabilis strains are poorly identified among UTI patients. The aims of the present study were to determine the prevalence of the uropathogenic P. mirabilis strains isolated from UTI patients by the detection of several P. mirabilis virulence genes and to characterize the antibiotic susceptibility profile of P. mirabilis isolates. P. mirabilis isolates were collected from urine specimens of patients suffering from UTI. Virulence genes in P. mirabilis, namely, hpmA, hpmB, rsbA, luxS, ureC1, hlyA, rpoA, atfA, atfC, mrpA, and pm1 were detected in the isolates via PCR detection method. All P. mirabilis virulence genes were detected in more than 90% of the isolates except hlyA gene, which was detected in only 23.8% of the isolates. The rate of susceptibility for ceftriaxone was 96.8%, followed by norfloxacin (82.5%), gentamicin (71.4%), ciprofloxacin (69.8%), cephalexin (52.4%), nalidixic acid (42.9%), sulfamethoxazole (39.7%), ampicillin (36.5%), and nitrofurantoin (3.2%). Significant associations (P<0.05) were detected between antimicrobial susceptibility of each of the following antibiotics and the presence virulence genes. Cephalexin antimicrobial susceptibility was significantly associated with the presence each of ureC1 and atfC. Sulfamethoxazole antimicrobial susceptibility was significantly associated with the presence atfA. Ceftriaxone antimicrobial susceptibility was significantly associated with the presence each of hpmA, ureC1, rpoA, atfC, mrpA, and pm1. Nitrofurantoin antimicrobial susceptibility was significantly associated with the presence each of hpmA, ureC1, rpoA, atfA, atfC, mrpA, and pm1. In conclusion, an association between the presence of urovirulence genes of P. mirabilis and increasing P. mirabilis resistance to antimicrobials has been demonstrated.http://dx.doi.org/10.1155/2020/1231807 |
| spellingShingle | Emad I. Hussein Khalid Al-Batayneh Majed M. Masadeh Fatina W. Dahadhah Mazhar Salim Al Zoubi Alaa A. Aljabali Karem H. Alzoubi Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection International Journal of Microbiology |
| title | Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection |
| title_full | Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection |
| title_fullStr | Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection |
| title_full_unstemmed | Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection |
| title_short | Assessment of Pathogenic Potential, Virulent Genes Profile, and Antibiotic Susceptibility of Proteus mirabilis from Urinary Tract Infection |
| title_sort | assessment of pathogenic potential virulent genes profile and antibiotic susceptibility of proteus mirabilis from urinary tract infection |
| url | http://dx.doi.org/10.1155/2020/1231807 |
| work_keys_str_mv | AT emadihussein assessmentofpathogenicpotentialvirulentgenesprofileandantibioticsusceptibilityofproteusmirabilisfromurinarytractinfection AT khalidalbatayneh assessmentofpathogenicpotentialvirulentgenesprofileandantibioticsusceptibilityofproteusmirabilisfromurinarytractinfection AT majedmmasadeh assessmentofpathogenicpotentialvirulentgenesprofileandantibioticsusceptibilityofproteusmirabilisfromurinarytractinfection AT fatinawdahadhah assessmentofpathogenicpotentialvirulentgenesprofileandantibioticsusceptibilityofproteusmirabilisfromurinarytractinfection AT mazharsalimalzoubi assessmentofpathogenicpotentialvirulentgenesprofileandantibioticsusceptibilityofproteusmirabilisfromurinarytractinfection AT alaaaaljabali assessmentofpathogenicpotentialvirulentgenesprofileandantibioticsusceptibilityofproteusmirabilisfromurinarytractinfection AT karemhalzoubi assessmentofpathogenicpotentialvirulentgenesprofileandantibioticsusceptibilityofproteusmirabilisfromurinarytractinfection |