Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors
We have previously reported that NS-398, a cyclooxygenase-2 (COX-2)–selective inhibitor, inhibited replicative cellular senescence in human dermal fibroblasts and skin aging in hairless mice. In contrast, celecoxib, another COX-2–selective inhibitor, and aspirin, a non-selective COX inhibitor, accel...
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BioMed Central
2017-06-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gni-15-56.pdf |
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| author | Jeong A. Han Jong-Il Kim |
| author_facet | Jeong A. Han Jong-Il Kim |
| author_sort | Jeong A. Han |
| collection | DOAJ |
| description | We have previously reported that NS-398, a cyclooxygenase-2 (COX-2)–selective inhibitor, inhibited replicative cellular senescence in human dermal fibroblasts and skin aging in hairless mice. In contrast, celecoxib, another COX-2–selective inhibitor, and aspirin, a non-selective COX inhibitor, accelerated the senescence and aging. To figure out causal factors for the senescence-modulating effect of the inhibitors, we here performed cDNA microarray experiment and subsequent Gene Set Enrichment Analysis. The data showed that several senescence-related gene sets were regulated by the inhibitor treatment. NS-398 up-regulated gene sets involved in the tumor necrosis factor β receptor pathway and the fructose and mannose metabolism, whereas it down-regulated a gene set involved in protein secretion. Celecoxib up-regulated gene sets involved in G2M checkpoint and E2F targets. Aspirin up-regulated the gene set involved in protein secretion, and down-regulated gene sets involved in RNA transcription. These results suggest that COX inhibitors modulate cellular senescence by different mechanisms and will provide useful information to understand senescence-modulating mechanisms of COX inhibitors. |
| format | Article |
| id | doaj-art-34a5feb31e7e4fba8d1b92c04fb4f3d1 |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | BioMed Central |
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| series | Genomics & Informatics |
| spelling | doaj-art-34a5feb31e7e4fba8d1b92c04fb4f3d12025-02-02T06:17:17ZengBioMed CentralGenomics & Informatics1598-866X2234-07422017-06-01152566410.5808/GI.2017.15.2.56210Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX InhibitorsJeong A. Han0Jong-Il Kim1Department of Biochemistry and Molecular Biology, Kangwon National University School of Medicine, Chuncheon 24341, Korea.Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul 03080, Korea.We have previously reported that NS-398, a cyclooxygenase-2 (COX-2)–selective inhibitor, inhibited replicative cellular senescence in human dermal fibroblasts and skin aging in hairless mice. In contrast, celecoxib, another COX-2–selective inhibitor, and aspirin, a non-selective COX inhibitor, accelerated the senescence and aging. To figure out causal factors for the senescence-modulating effect of the inhibitors, we here performed cDNA microarray experiment and subsequent Gene Set Enrichment Analysis. The data showed that several senescence-related gene sets were regulated by the inhibitor treatment. NS-398 up-regulated gene sets involved in the tumor necrosis factor β receptor pathway and the fructose and mannose metabolism, whereas it down-regulated a gene set involved in protein secretion. Celecoxib up-regulated gene sets involved in G2M checkpoint and E2F targets. Aspirin up-regulated the gene set involved in protein secretion, and down-regulated gene sets involved in RNA transcription. These results suggest that COX inhibitors modulate cellular senescence by different mechanisms and will provide useful information to understand senescence-modulating mechanisms of COX inhibitors.http://genominfo.org/upload/pdf/gni-15-56.pdfcyclooxygenase 2fibroblastgene set enrichment analysisinhibitorsenescence |
| spellingShingle | Jeong A. Han Jong-Il Kim Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors Genomics & Informatics cyclooxygenase 2 fibroblast gene set enrichment analysis inhibitor senescence |
| title | Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors |
| title_full | Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors |
| title_fullStr | Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors |
| title_full_unstemmed | Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors |
| title_short | Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors |
| title_sort | analysis of gene expression in human dermal fibroblasts treated with senescence modulating cox inhibitors |
| topic | cyclooxygenase 2 fibroblast gene set enrichment analysis inhibitor senescence |
| url | http://genominfo.org/upload/pdf/gni-15-56.pdf |
| work_keys_str_mv | AT jeongahan analysisofgeneexpressioninhumandermalfibroblaststreatedwithsenescencemodulatingcoxinhibitors AT jongilkim analysisofgeneexpressioninhumandermalfibroblaststreatedwithsenescencemodulatingcoxinhibitors |