D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils
Abstract Background Periodontitis is a chronic inflammatory disease characterized by the destruction of the components of the periodontium. It significantly impacts oral health and has been linked to systemic conditions like cardiovascular disease and diabetes. The critical role of neutrophils in th...
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BMC
2024-11-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-05080-1 |
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| author | Xue Li Xueting Chen Qingyu Zhu Pengye Zhang Shunxue Nan Lei Lv Shengcai Qi |
| author_facet | Xue Li Xueting Chen Qingyu Zhu Pengye Zhang Shunxue Nan Lei Lv Shengcai Qi |
| author_sort | Xue Li |
| collection | DOAJ |
| description | Abstract Background Periodontitis is a chronic inflammatory disease characterized by the destruction of the components of the periodontium. It significantly impacts oral health and has been linked to systemic conditions like cardiovascular disease and diabetes. The critical role of neutrophils in the occurrence and development of chronic periodontitis has been paid increasing attention. The study aimed to explore the protective effects of D-mannose on chronic periodontitis and determine whether its underlying mechanisms is related to neutrophils. Methods To explore the protective effects of D-mannose on chronic periodontitis, the eight-week-old Sprague Dawley rat model of lipopolysaccharide (LPS)-induced periodontitis was established, followed by D-mannose treatment by oral gavage. To evaluate the protective effects of D-mannose against periodontal bone loss, methylene blue staining, hematoxylin and eosin (H&E) staining, and micro-CT scanning were utilized. Then, immunofluorescence (IF), Western Blot, and RT-PCR were applied to assess the expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and IL-17), anti-inflammatory cytokine (IL-10), tumor necrosis factor-alpha (TNF-α), granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), ten-eleven translocation 2 (TET2), and key glycolytic enzymes (HK1, HK2, PFKFB3), and to examine D-mannose’s impact on the recruitment and activation of neutrophils in the gingiva. Additionally, neutrophils isolated from the peripheral blood of healthy rats were treated with LPS and D-mannose, and changes in the expression levels of myeloperoxidase (MPO), IL-1β, IL-6, IL-17, IL-10, and TET2 were observed via IF. Results In vivo, D-mannose inhibited LPS-induced alveolar bone resorption in rats. After D-mannose treatment, the expression levels of IL-17 (p<0.01) and TET2 (p<0.01) were suppressed by IF, and the expression levels of IL-1β (p<0.05), IL-17 (p<0.05) and TET2 (p<0.01) were downregulated by WB. The results of qPCR showed that D-mannose reduced the expression levels of IL-1β (p<0.05), IL-6 (p<0.01), IL-17 (p<0.01), TNF-α (p<0.01), G-CSF (p<0.01), GM-CSF (p<0.01), TET2 (p<0.01), HK1 (p<0.01), HK2 (p<0.01), and PFKFB3 (p<0.01). D-mannose also inhibited the recruitment and activation of neutrophils in LPS-treated rat gingival tissues. In vitro, the results of IF showed that D-mannose inhibited the activation of neutrophils stimulated by LPS, downregulated the expression of IL-1β (p < 0.05), IL-6, IL-17 (p < 0.01), and TET2 (p < 0.01), and upregulated the expression of IL-10 (p < 0.01). Conclusions D-mannose can alleviate chronic periodontitis in rats by regulating the functions of neutrophils, potentially associated with the expression of TET2 and glycolysis, providing new insights into the potential application of D-mannose to chronic periodontitis. |
| format | Article |
| id | doaj-art-349f458821134201973b0d1cec3bb153 |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-349f458821134201973b0d1cec3bb1532025-02-02T12:45:30ZengBMCBMC Oral Health1472-68312024-11-0124111410.1186/s12903-024-05080-1D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophilsXue Li0Xueting Chen1Qingyu Zhu2Pengye Zhang3Shunxue Nan4Lei Lv5Shengcai Qi6Department of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityDepartment of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityMinistry of Education Key Laboratory of Metabolism and Molecular Medicine, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan UniversityDepartment of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Fudan UniversityAbstract Background Periodontitis is a chronic inflammatory disease characterized by the destruction of the components of the periodontium. It significantly impacts oral health and has been linked to systemic conditions like cardiovascular disease and diabetes. The critical role of neutrophils in the occurrence and development of chronic periodontitis has been paid increasing attention. The study aimed to explore the protective effects of D-mannose on chronic periodontitis and determine whether its underlying mechanisms is related to neutrophils. Methods To explore the protective effects of D-mannose on chronic periodontitis, the eight-week-old Sprague Dawley rat model of lipopolysaccharide (LPS)-induced periodontitis was established, followed by D-mannose treatment by oral gavage. To evaluate the protective effects of D-mannose against periodontal bone loss, methylene blue staining, hematoxylin and eosin (H&E) staining, and micro-CT scanning were utilized. Then, immunofluorescence (IF), Western Blot, and RT-PCR were applied to assess the expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and IL-17), anti-inflammatory cytokine (IL-10), tumor necrosis factor-alpha (TNF-α), granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), ten-eleven translocation 2 (TET2), and key glycolytic enzymes (HK1, HK2, PFKFB3), and to examine D-mannose’s impact on the recruitment and activation of neutrophils in the gingiva. Additionally, neutrophils isolated from the peripheral blood of healthy rats were treated with LPS and D-mannose, and changes in the expression levels of myeloperoxidase (MPO), IL-1β, IL-6, IL-17, IL-10, and TET2 were observed via IF. Results In vivo, D-mannose inhibited LPS-induced alveolar bone resorption in rats. After D-mannose treatment, the expression levels of IL-17 (p<0.01) and TET2 (p<0.01) were suppressed by IF, and the expression levels of IL-1β (p<0.05), IL-17 (p<0.05) and TET2 (p<0.01) were downregulated by WB. The results of qPCR showed that D-mannose reduced the expression levels of IL-1β (p<0.05), IL-6 (p<0.01), IL-17 (p<0.01), TNF-α (p<0.01), G-CSF (p<0.01), GM-CSF (p<0.01), TET2 (p<0.01), HK1 (p<0.01), HK2 (p<0.01), and PFKFB3 (p<0.01). D-mannose also inhibited the recruitment and activation of neutrophils in LPS-treated rat gingival tissues. In vitro, the results of IF showed that D-mannose inhibited the activation of neutrophils stimulated by LPS, downregulated the expression of IL-1β (p < 0.05), IL-6, IL-17 (p < 0.01), and TET2 (p < 0.01), and upregulated the expression of IL-10 (p < 0.01). Conclusions D-mannose can alleviate chronic periodontitis in rats by regulating the functions of neutrophils, potentially associated with the expression of TET2 and glycolysis, providing new insights into the potential application of D-mannose to chronic periodontitis.https://doi.org/10.1186/s12903-024-05080-1Chronic periodontitisMannoseNeutrophils |
| spellingShingle | Xue Li Xueting Chen Qingyu Zhu Pengye Zhang Shunxue Nan Lei Lv Shengcai Qi D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils BMC Oral Health Chronic periodontitis Mannose Neutrophils |
| title | D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils |
| title_full | D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils |
| title_fullStr | D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils |
| title_full_unstemmed | D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils |
| title_short | D-mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils |
| title_sort | d mannose alleviates chronic periodontitis in rats by regulating the functions of neutrophils |
| topic | Chronic periodontitis Mannose Neutrophils |
| url | https://doi.org/10.1186/s12903-024-05080-1 |
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