Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy Schoolchildren
Background. The use of easily accessible biomarkers for assessing young patients’ health is weighty. This cohort study is aimed at measuring stress/immune biomarkers in the saliva of healthy school-age children and comparing subgroups according to age, sex, and stress perception. Material and Method...
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/3639441 |
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| author | Anna Krahel Elzbieta Paszynska Justyna Otulakowska-Skrzynska Szymon Rzatowski Amadeusz Hernik Agnieszka Slopien Tomasz Hanć Paula Szczesniewska Ewa Bryl Yves Boucher Marta Tyszkiewicz-Nwafor Maria Gawriolek Monika Dmitrzak-Weglarz |
| author_facet | Anna Krahel Elzbieta Paszynska Justyna Otulakowska-Skrzynska Szymon Rzatowski Amadeusz Hernik Agnieszka Slopien Tomasz Hanć Paula Szczesniewska Ewa Bryl Yves Boucher Marta Tyszkiewicz-Nwafor Maria Gawriolek Monika Dmitrzak-Weglarz |
| author_sort | Anna Krahel |
| collection | DOAJ |
| description | Background. The use of easily accessible biomarkers for assessing young patients’ health is weighty. This cohort study is aimed at measuring stress/immune biomarkers in the saliva of healthy school-age children and comparing subgroups according to age, sex, and stress perception. Material and Methods. 503 children under 12 years old (8.7±1.3) were included with anthropometric evaluation (height, waist, hip circumference, body weight, and body mass index (BMI)). Levels of opiorphin (OPI), free cortisol, alpha-amylase (sAA), and secreted immunoglobulin (sIgA) were determined by quantitative assays (ELISA) in unstimulated saliva. Unpaired t-test, Welch test, and Mann–Whitney U test were applied for appropriate group comparisons, and the correlation between variables was analyzed with Spearman’s rank coefficient. Results were considered significant at p<0.05. Results. sIgA and sAA exhibited significant differences depending on age and sex: IgA (ng/mL): 86±68.6 vs. 104.9±72.1 for (6-7 y.o.) and (8-11 y.o.), respectively, and 108.1±80.1 vs. 94.6±62.2 for male and females, respectively; sAA (U/mL): 78.9±54.4 vs. 100.5±81.2 for (6-7 y.o.) and (8-11 y.o.). No difference related to age or sex between groups was observed for cortisol and OPI. However, OPI levels were higher and correlated to prior stress exposure in children (0.31±0.4 vs. 0.26±0.5 ng/mL, p=0.031). sAA was negatively correlated to low mood self-declaration in children in the last two weeks (r=−0.10, p=0.045). Conclusions. sIgA and sAA can be used as sex- and age-related biomarkers in children 6-12 y.o., which is not the case for free cortisol and opiorphin. However, OPI reflected previous exposure to stress, suggesting its use for evaluating stress-related changes in children |
| format | Article |
| id | doaj-art-3465b4379f034133bc20f75fdf3b0228 |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-3465b4379f034133bc20f75fdf3b02282025-02-03T06:46:18ZengWileyMediators of Inflammation1466-18612021-01-01202110.1155/2021/3639441Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy SchoolchildrenAnna Krahel0Elzbieta Paszynska1Justyna Otulakowska-Skrzynska2Szymon Rzatowski3Amadeusz Hernik4Agnieszka Slopien5Tomasz Hanć6Paula Szczesniewska7Ewa Bryl8Yves Boucher9Marta Tyszkiewicz-Nwafor10Maria Gawriolek11Monika Dmitrzak-Weglarz12Department of Integrated DentistryDepartment of Integrated DentistryDepartment of Integrated DentistryDepartment of Integrated DentistryDepartment of Integrated DentistryDepartment of Child and Adolescent PsychiatryInstitute of Human Biology and EvolutionInstitute of Human Biology and EvolutionInstitute of Human Biology and EvolutionLaboratoire de Neurobiologie Orofaciale (EA 7543)Department of Child and Adolescent PsychiatryDepartment of Integrated DentistryDepartment of Psychiatric GeneticsBackground. The use of easily accessible biomarkers for assessing young patients’ health is weighty. This cohort study is aimed at measuring stress/immune biomarkers in the saliva of healthy school-age children and comparing subgroups according to age, sex, and stress perception. Material and Methods. 503 children under 12 years old (8.7±1.3) were included with anthropometric evaluation (height, waist, hip circumference, body weight, and body mass index (BMI)). Levels of opiorphin (OPI), free cortisol, alpha-amylase (sAA), and secreted immunoglobulin (sIgA) were determined by quantitative assays (ELISA) in unstimulated saliva. Unpaired t-test, Welch test, and Mann–Whitney U test were applied for appropriate group comparisons, and the correlation between variables was analyzed with Spearman’s rank coefficient. Results were considered significant at p<0.05. Results. sIgA and sAA exhibited significant differences depending on age and sex: IgA (ng/mL): 86±68.6 vs. 104.9±72.1 for (6-7 y.o.) and (8-11 y.o.), respectively, and 108.1±80.1 vs. 94.6±62.2 for male and females, respectively; sAA (U/mL): 78.9±54.4 vs. 100.5±81.2 for (6-7 y.o.) and (8-11 y.o.). No difference related to age or sex between groups was observed for cortisol and OPI. However, OPI levels were higher and correlated to prior stress exposure in children (0.31±0.4 vs. 0.26±0.5 ng/mL, p=0.031). sAA was negatively correlated to low mood self-declaration in children in the last two weeks (r=−0.10, p=0.045). Conclusions. sIgA and sAA can be used as sex- and age-related biomarkers in children 6-12 y.o., which is not the case for free cortisol and opiorphin. However, OPI reflected previous exposure to stress, suggesting its use for evaluating stress-related changes in childrenhttp://dx.doi.org/10.1155/2021/3639441 |
| spellingShingle | Anna Krahel Elzbieta Paszynska Justyna Otulakowska-Skrzynska Szymon Rzatowski Amadeusz Hernik Agnieszka Slopien Tomasz Hanć Paula Szczesniewska Ewa Bryl Yves Boucher Marta Tyszkiewicz-Nwafor Maria Gawriolek Monika Dmitrzak-Weglarz Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy Schoolchildren Mediators of Inflammation |
| title | Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy Schoolchildren |
| title_full | Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy Schoolchildren |
| title_fullStr | Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy Schoolchildren |
| title_full_unstemmed | Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy Schoolchildren |
| title_short | Salivary Biomarkers (Opiorphin, Cortisol, Amylase, and IgA) Related to Age, Sex, and Stress Perception in a Prospective Cohort of Healthy Schoolchildren |
| title_sort | salivary biomarkers opiorphin cortisol amylase and iga related to age sex and stress perception in a prospective cohort of healthy schoolchildren |
| url | http://dx.doi.org/10.1155/2021/3639441 |
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