Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes
Poly(N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes (SWNTs) was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2016/3539609 |
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| _version_ | 1832556544442499072 |
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| author | Kazuo Umemura Katsuki Izumi Yoshikazu Kumashiro Shusuke Oura Teruo Okano |
| author_facet | Kazuo Umemura Katsuki Izumi Yoshikazu Kumashiro Shusuke Oura Teruo Okano |
| author_sort | Kazuo Umemura |
| collection | DOAJ |
| description | Poly(N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes (SWNTs) was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto the hybrid surfaces at 37°C, even in the absence of DNA molecules, the adsorption of RecA was inhibited at 4°C. These results suggest that the thermoresponsive functions of PNIPAAm were effective, even on the SWNT surfaces, which supports the possibility of developing nanobiodevices using PNIPAAm-SWNT hybrids. However, although RecA is a DNA binding protein, there was no significant difference in the adsorption of RecA onto PNIPAAm-SWNT surfaces with and without DNA molecules. This study provides fundamental information for potential biological applications of PNIPAAm-SWNT hybrids. |
| format | Article |
| id | doaj-art-3458e3aa34444b1d8280bdfeb1c4cde1 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-3458e3aa34444b1d8280bdfeb1c4cde12025-02-03T05:45:04ZengWileyInternational Journal of Polymer Science1687-94221687-94302016-01-01201610.1155/2016/35396093539609Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon NanotubesKazuo Umemura0Katsuki Izumi1Yoshikazu Kumashiro2Shusuke Oura3Teruo Okano4Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, JapanGraduate School of Mathematics and Science Education, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 1620054, JapanDepartment of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, JapanInstitute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 1620054, JapanPoly(N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes (SWNTs) was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto the hybrid surfaces at 37°C, even in the absence of DNA molecules, the adsorption of RecA was inhibited at 4°C. These results suggest that the thermoresponsive functions of PNIPAAm were effective, even on the SWNT surfaces, which supports the possibility of developing nanobiodevices using PNIPAAm-SWNT hybrids. However, although RecA is a DNA binding protein, there was no significant difference in the adsorption of RecA onto PNIPAAm-SWNT surfaces with and without DNA molecules. This study provides fundamental information for potential biological applications of PNIPAAm-SWNT hybrids.http://dx.doi.org/10.1155/2016/3539609 |
| spellingShingle | Kazuo Umemura Katsuki Izumi Yoshikazu Kumashiro Shusuke Oura Teruo Okano Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes International Journal of Polymer Science |
| title | Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes |
| title_full | Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes |
| title_fullStr | Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes |
| title_full_unstemmed | Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes |
| title_short | Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes |
| title_sort | protein adsorption on hybrids of thermoresponsive polymers and single walled carbon nanotubes |
| url | http://dx.doi.org/10.1155/2016/3539609 |
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