Protein Adsorption on Hybrids of Thermoresponsive Polymers and Single-Walled Carbon Nanotubes
Poly(N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes (SWNTs) was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2016/3539609 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Poly(N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes (SWNTs) was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto the hybrid surfaces at 37°C, even in the absence of DNA molecules, the adsorption of RecA was inhibited at 4°C. These results suggest that the thermoresponsive functions of PNIPAAm were effective, even on the SWNT surfaces, which supports the possibility of developing nanobiodevices using PNIPAAm-SWNT hybrids. However, although RecA is a DNA binding protein, there was no significant difference in the adsorption of RecA onto PNIPAAm-SWNT surfaces with and without DNA molecules. This study provides fundamental information for potential biological applications of PNIPAAm-SWNT hybrids. |
|---|---|
| ISSN: | 1687-9422 1687-9430 |