Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress
Glutathione S-transferases (GSTs) are ancient proteins encoded by a large gene family in plants, which play multiple roles in plant growth and development. However, there has been little study on the GST genes of common wheat (Triticum aestivum) and its relatives (Triticum durum, Triticum urartu, an...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2021/6289174 |
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| author | Yongchao Hao Shoushen Xu Zhongfan lyu Hongwei Wang Lingrang Kong Silong Sun |
| author_facet | Yongchao Hao Shoushen Xu Zhongfan lyu Hongwei Wang Lingrang Kong Silong Sun |
| author_sort | Yongchao Hao |
| collection | DOAJ |
| description | Glutathione S-transferases (GSTs) are ancient proteins encoded by a large gene family in plants, which play multiple roles in plant growth and development. However, there has been little study on the GST genes of common wheat (Triticum aestivum) and its relatives (Triticum durum, Triticum urartu, and Aegilops tauschii), which are four important species of Triticeae. Here, a genome-wide comprehensive analysis of this gene family was performed on the genomes of common wheat and its relatives. A total of 346 GST genes in T. aestivum, 226 in T. durum, 104 in T. urartu, and 105 in Ae. tauschii were identified, and all members were divided into ten classes. Transcriptome analysis was used to identify GST genes that respond to salt stress in common wheat, which revealed that the reaction of GST genes is not sensitive to low and moderate salt concentrations but is sensitive to severe concentrations of the stressor, and the GST genes related to salt stress mainly come from the Tau and Phi classes. Six GST genes which respond to different salt concentrations were selected and validated by a qRT-PCR assay. These findings will not only provide helpful information about the function of GST genes in Triticeae species but also offer insights for the future application of salt stress resistance breeding in common wheat. |
| format | Article |
| id | doaj-art-3451bfe0d7d64d009534917ce55b8653 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-3451bfe0d7d64d009534917ce55b86532025-02-03T01:10:53ZengWileyInternational Journal of Genomics2314-436X2314-43782021-01-01202110.1155/2021/62891746289174Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt StressYongchao Hao0Shoushen Xu1Zhongfan lyu2Hongwei Wang3Lingrang Kong4Silong Sun5State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, ChinaGlutathione S-transferases (GSTs) are ancient proteins encoded by a large gene family in plants, which play multiple roles in plant growth and development. However, there has been little study on the GST genes of common wheat (Triticum aestivum) and its relatives (Triticum durum, Triticum urartu, and Aegilops tauschii), which are four important species of Triticeae. Here, a genome-wide comprehensive analysis of this gene family was performed on the genomes of common wheat and its relatives. A total of 346 GST genes in T. aestivum, 226 in T. durum, 104 in T. urartu, and 105 in Ae. tauschii were identified, and all members were divided into ten classes. Transcriptome analysis was used to identify GST genes that respond to salt stress in common wheat, which revealed that the reaction of GST genes is not sensitive to low and moderate salt concentrations but is sensitive to severe concentrations of the stressor, and the GST genes related to salt stress mainly come from the Tau and Phi classes. Six GST genes which respond to different salt concentrations were selected and validated by a qRT-PCR assay. These findings will not only provide helpful information about the function of GST genes in Triticeae species but also offer insights for the future application of salt stress resistance breeding in common wheat.http://dx.doi.org/10.1155/2021/6289174 |
| spellingShingle | Yongchao Hao Shoushen Xu Zhongfan lyu Hongwei Wang Lingrang Kong Silong Sun Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress International Journal of Genomics |
| title | Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress |
| title_full | Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress |
| title_fullStr | Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress |
| title_full_unstemmed | Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress |
| title_short | Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress |
| title_sort | comparative analysis of the glutathione s transferase gene family of four triticeae species and transcriptome analysis of gst genes in common wheat responding to salt stress |
| url | http://dx.doi.org/10.1155/2021/6289174 |
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