Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology
Xenorhabdus stockiae PB09 bacterium has been shown to exhibit antifungal activity against several plant pathogens. To improve its efficacy, the optimization of the nutritional components in culture media was performed. The medium components that have significant effects on antifungal activity of X....
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| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/3965851 |
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| author | Chirayu Sa-uth Paweena Rattanasena Angsumarn Chandrapatya Prapassorn Bussaman |
| author_facet | Chirayu Sa-uth Paweena Rattanasena Angsumarn Chandrapatya Prapassorn Bussaman |
| author_sort | Chirayu Sa-uth |
| collection | DOAJ |
| description | Xenorhabdus stockiae PB09 bacterium has been shown to exhibit antifungal activity against several plant pathogens. To improve its efficacy, the optimization of the nutritional components in culture media was performed. The medium components that have significant effects on antifungal activity of X. stockiae PB09 were initially identified using a fractional factorial design. Response surface methodology and central composite design were then used to create a model for optimizing the levels of carbon, nitrogen, and mineral sources that maximize antifungal activity of X. stockiae PB09. After that, the suitable carbon, nitrogen, and mineral sources were selected and adjusted by the second-order polynomial regression model, which predicted that 98.62% of antifungal activity could be obtained when the medium contained sucrose, yeast extract, NaCl, and K2HPO4 at 3.24, 23.71, 5.46, and 2.73 g/L, respectively. Laboratory verification of this recipe resulted in the antifungal activity at 97.95% in the shake flask experiment after 48-hour cultivation, which was significantly 27.22% higher than that obtained by using the TSB medium. In addition, X. stockiae PB09 cultured in the verified recipe by using 5 L fermenter could effectively inhibit the mycelial growth of Phytophthora sp., Rhizoctonia solani, Pythium sp., and Fusarium oxysporum. This study demonstrated that the RSM and CCD were shown to be valuable tools for optimizing the culture medium that maximize the antifungal activity of X. stockiae PB09. |
| format | Article |
| id | doaj-art-34391c24c3d046ddaa995a8e5ae31120 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | International Journal of Microbiology |
| spelling | doaj-art-34391c24c3d046ddaa995a8e5ae311202025-02-03T05:52:46ZengWileyInternational Journal of Microbiology1687-918X1687-91982018-01-01201810.1155/2018/39658513965851Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface MethodologyChirayu Sa-uth0Paweena Rattanasena1Angsumarn Chandrapatya2Prapassorn Bussaman3Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, ThailandCommunity Public Health Sub-Department, Department of Applied Sciences, Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Ayutthaya 13000, ThailandDepartment of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, ThailandDepartment of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, ThailandXenorhabdus stockiae PB09 bacterium has been shown to exhibit antifungal activity against several plant pathogens. To improve its efficacy, the optimization of the nutritional components in culture media was performed. The medium components that have significant effects on antifungal activity of X. stockiae PB09 were initially identified using a fractional factorial design. Response surface methodology and central composite design were then used to create a model for optimizing the levels of carbon, nitrogen, and mineral sources that maximize antifungal activity of X. stockiae PB09. After that, the suitable carbon, nitrogen, and mineral sources were selected and adjusted by the second-order polynomial regression model, which predicted that 98.62% of antifungal activity could be obtained when the medium contained sucrose, yeast extract, NaCl, and K2HPO4 at 3.24, 23.71, 5.46, and 2.73 g/L, respectively. Laboratory verification of this recipe resulted in the antifungal activity at 97.95% in the shake flask experiment after 48-hour cultivation, which was significantly 27.22% higher than that obtained by using the TSB medium. In addition, X. stockiae PB09 cultured in the verified recipe by using 5 L fermenter could effectively inhibit the mycelial growth of Phytophthora sp., Rhizoctonia solani, Pythium sp., and Fusarium oxysporum. This study demonstrated that the RSM and CCD were shown to be valuable tools for optimizing the culture medium that maximize the antifungal activity of X. stockiae PB09.http://dx.doi.org/10.1155/2018/3965851 |
| spellingShingle | Chirayu Sa-uth Paweena Rattanasena Angsumarn Chandrapatya Prapassorn Bussaman Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology International Journal of Microbiology |
| title | Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology |
| title_full | Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology |
| title_fullStr | Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology |
| title_full_unstemmed | Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology |
| title_short | Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology |
| title_sort | modification of medium composition for enhancing the production of antifungal activity from xenorhabdus stockiae pb09 by using response surface methodology |
| url | http://dx.doi.org/10.1155/2018/3965851 |
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