SARS-CoV-2 Variants Screening in Burkina Faso
Introduction: In Sub-Saharan Africa, the data on the mutations and variants of circulating SARS-CoV-2 is limited. This study aimed to screen specific mutations and variants of SARS-CoV-2 circulating in Burkina Faso. Methods: This study included symptomatic and asymptomatic individuals who underwe...
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Pasteur Institute of Iran
2022-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| author | Serge Théophile Soubeiga1* Kinda Charlotte2 Abdou Azaque Zoure1 Tegwinde Rebeca Compaoré1 Sylvie Zida1 Alidou Kagambega1 Tani Sagna1 Charlemagne Dabiré1 Oumarou Ouedraogo1 Nadiyah Lasisi2 Alexia Kabore2 Farida Milène Zida1 Binta Fofana1 Stéphane Somé2 Abdoul Nikiema1 Dinanibè Kambire1 Dramane Zongo1 Issiaka Soulama1 Charles Sawadogo3 Sandrine Gampini4 Henri Gautier Ouedraogo1 |
| author_facet | Serge Théophile Soubeiga1* Kinda Charlotte2 Abdou Azaque Zoure1 Tegwinde Rebeca Compaoré1 Sylvie Zida1 Alidou Kagambega1 Tani Sagna1 Charlemagne Dabiré1 Oumarou Ouedraogo1 Nadiyah Lasisi2 Alexia Kabore2 Farida Milène Zida1 Binta Fofana1 Stéphane Somé2 Abdoul Nikiema1 Dinanibè Kambire1 Dramane Zongo1 Issiaka Soulama1 Charles Sawadogo3 Sandrine Gampini4 Henri Gautier Ouedraogo1 |
| author_sort | Serge Théophile Soubeiga1* |
| collection | DOAJ |
| description | Introduction: In Sub-Saharan Africa, the data on the mutations and variants of
circulating SARS-CoV-2 is limited. This study aimed to screen specific
mutations and variants of SARS-CoV-2 circulating in Burkina Faso. Methods:
This study included symptomatic and asymptomatic individuals who underwent
diagnostic testing for SARS-CoV-2 by RT-PCR on nasopharyngeal and
oropharyngeal swabs from 7 December 2021 to 12 January 2022. Samples from
individuals with a Ct value ≤ 33 were selected for the variants-specific mutation
screening. The screening was performed using two kits, “SNPsig® SARS-COV-
2 (Escape PLEX)" and "SNPsig® VariPLEX™ (COVID-19) Real-Time PCR
Assay". Results: SARS-CoV-2 prevalence was 18.9% (332/1758). A total of
113 samples (34.04%) had a Ct value less than (≤ 33), with only 20.35%
(23/113) belonging to symptomatic patients. The mean age was 39.01±13 years.
The Beta variant (B.1.351) was the most detected one comprising 78.8%
(89/113) of variants. Gamma and Delta variants were detected at a low
proportion of 0.9% (1/113). No mutation or variant was detected in seven (6.2%)
samples. Conclusion: Specific mutation screening detected Beta (B.1.351),
Gamma (P.1), and Delta (B.1.617.2) variants of SARS-CoV-2 circulating in
Burkina Faso. The absence of mutations in some samples might suggest variants
other than those detected. |
| format | Article |
| id | doaj-art-342f55d4ffff40c88da26921f16f128b |
| institution | OA Journals |
| issn | 2345-5349 2345-5330 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj-art-342f55d4ffff40c88da26921f16f128b2025-08-20T02:28:41ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302022-09-0110313514010.52547/JoMMID.10.3.135SARS-CoV-2 Variants Screening in Burkina FasoSerge Théophile Soubeiga1*0https://orcid.org/0000-0002-9741-2428Kinda Charlotte21Abdou Azaque Zoure12https://orcid.org/0000-0002-9423-024XTegwinde Rebeca Compaoré13https://orcid.org/0000-0002-5956-3444Sylvie Zida14https://orcid.org/0000-0003-1727-2291Alidou Kagambega15Tani Sagna16https://orcid.org/0000-0002-8775-0693Charlemagne Dabiré17https://orcid.org/0000-0003-0160-8141Oumarou Ouedraogo18https://orcid.org/0000-0002-6230-7126Nadiyah Lasisi29Alexia Kabore210Farida Milène Zida111Binta Fofana112Stéphane Somé213https://orcid.org/0000-0002-0630-6460Abdoul Nikiema114Dinanibè Kambire115https://orcid.org/0000-0001-9274-1428Dramane Zongo116https://orcid.org/0000-0003-3097-1142Issiaka Soulama117https://orcid.org/0000-0002-0993-0525Charles Sawadogo318Sandrine Gampini419Henri Gautier Ouedraogo120https://orcid.org/0000-0001-8566-75301Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso²University Joseph Ki-Zerbo, Laboratory of Molecular Biology and Genetics (LABIOGENE), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso²University Joseph Ki-Zerbo, Laboratory of Molecular Biology and Genetics (LABIOGENE), Ouagadougou, Burkina Faso²University Joseph Ki-Zerbo, Laboratory of Molecular Biology and Genetics (LABIOGENE), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso²University Joseph Ki-Zerbo, Laboratory of Molecular Biology and Genetics (LABIOGENE), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso3Ministère de la santé et de l’Hygiène publique, Direction des Laboratoires de Biologie Médicale (DLBM), Ouagadougou, Burkina Faso4World Health Organization (WHO), Ouagadougou, Burkina Faso1Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina FasoIntroduction: In Sub-Saharan Africa, the data on the mutations and variants of circulating SARS-CoV-2 is limited. This study aimed to screen specific mutations and variants of SARS-CoV-2 circulating in Burkina Faso. Methods: This study included symptomatic and asymptomatic individuals who underwent diagnostic testing for SARS-CoV-2 by RT-PCR on nasopharyngeal and oropharyngeal swabs from 7 December 2021 to 12 January 2022. Samples from individuals with a Ct value ≤ 33 were selected for the variants-specific mutation screening. The screening was performed using two kits, “SNPsig® SARS-COV- 2 (Escape PLEX)" and "SNPsig® VariPLEX™ (COVID-19) Real-Time PCR Assay". Results: SARS-CoV-2 prevalence was 18.9% (332/1758). A total of 113 samples (34.04%) had a Ct value less than (≤ 33), with only 20.35% (23/113) belonging to symptomatic patients. The mean age was 39.01±13 years. The Beta variant (B.1.351) was the most detected one comprising 78.8% (89/113) of variants. Gamma and Delta variants were detected at a low proportion of 0.9% (1/113). No mutation or variant was detected in seven (6.2%) samples. Conclusion: Specific mutation screening detected Beta (B.1.351), Gamma (P.1), and Delta (B.1.617.2) variants of SARS-CoV-2 circulating in Burkina Faso. The absence of mutations in some samples might suggest variants other than those detected.sars-cov-2rt-pcrctvariantmutationburkina faso |
| spellingShingle | Serge Théophile Soubeiga1* Kinda Charlotte2 Abdou Azaque Zoure1 Tegwinde Rebeca Compaoré1 Sylvie Zida1 Alidou Kagambega1 Tani Sagna1 Charlemagne Dabiré1 Oumarou Ouedraogo1 Nadiyah Lasisi2 Alexia Kabore2 Farida Milène Zida1 Binta Fofana1 Stéphane Somé2 Abdoul Nikiema1 Dinanibè Kambire1 Dramane Zongo1 Issiaka Soulama1 Charles Sawadogo3 Sandrine Gampini4 Henri Gautier Ouedraogo1 SARS-CoV-2 Variants Screening in Burkina Faso Journal of Medical Microbiology and Infectious Diseases sars-cov-2 rt-pcr ct variant mutation burkina faso |
| title | SARS-CoV-2 Variants Screening in Burkina Faso |
| title_full | SARS-CoV-2 Variants Screening in Burkina Faso |
| title_fullStr | SARS-CoV-2 Variants Screening in Burkina Faso |
| title_full_unstemmed | SARS-CoV-2 Variants Screening in Burkina Faso |
| title_short | SARS-CoV-2 Variants Screening in Burkina Faso |
| title_sort | sars cov 2 variants screening in burkina faso |
| topic | sars-cov-2 rt-pcr ct variant mutation burkina faso |
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