Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical Applications
Hydrogels are a viable option for biomedical applications due to their biocompatibility, biodegradability, and ability to incorporate various healing agents while maintaining their biological efficacy. This study focused on the preparation and characterization of novel hybrid hydrogels enriched with...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2310-2861/11/1/17 |
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| author | Dana Stan Andreea-Cristina Mirica Sorin Mocanu Diana Stan Iunia Podolean Natalia Candu Magdi El Fergani Laura Mihaela Stefan Ana-Maria Seciu-Grama Ludmila Aricov Oana Brincoveanu Carmen Moldovan Lorena-Andreea Bocancia-Mateescu Simona M. Coman |
| author_facet | Dana Stan Andreea-Cristina Mirica Sorin Mocanu Diana Stan Iunia Podolean Natalia Candu Magdi El Fergani Laura Mihaela Stefan Ana-Maria Seciu-Grama Ludmila Aricov Oana Brincoveanu Carmen Moldovan Lorena-Andreea Bocancia-Mateescu Simona M. Coman |
| author_sort | Dana Stan |
| collection | DOAJ |
| description | Hydrogels are a viable option for biomedical applications due to their biocompatibility, biodegradability, and ability to incorporate various healing agents while maintaining their biological efficacy. This study focused on the preparation and characterization of novel hybrid hydrogels enriched with the natural algae compound Ulvan for potential use in wound dressings. The characterization of the hydrogel membranes involved multiple methods to assess their structural, mechanical, and chemical properties, such as pH measurements, swelling, moisture content and uptake, gel fraction, hydrolytic degradation, protein adsorption and denaturation tests, rheological measurements, SEM, biocompatibility testing, and scratch wound assay. The hydrogel obtained with a higher concentration of Ulvan (1 mg/mL) exhibited superior mechanical properties, a swelling index of 264%, a water content of 55%, and a lower degradation percentage. In terms of rheological properties, the inclusion of ULV in the hydrogel composition enhanced gel strength, and the Alginate + PVA + 1.0ULV sample demonstrated the greatest resistance to deformation. All hydrogels exhibited good biocompatibility, with cell viability above 70% and no obvious morphological modifications. The addition of Ulvan potentiates the regenerative effect of hydrogel membranes. Subsequent studies will focus on encapsulating bioactive compounds, investigating their release behavior, and evaluating their active biological effects. |
| format | Article |
| id | doaj-art-342df1ecd1c9490bb68acd355ad5bf08 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-342df1ecd1c9490bb68acd355ad5bf082025-01-24T13:33:47ZengMDPI AGGels2310-28612024-12-011111710.3390/gels11010017Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical ApplicationsDana Stan0Andreea-Cristina Mirica1Sorin Mocanu2Diana Stan3Iunia Podolean4Natalia Candu5Magdi El Fergani6Laura Mihaela Stefan7Ana-Maria Seciu-Grama8Ludmila Aricov9Oana Brincoveanu10Carmen Moldovan11Lorena-Andreea Bocancia-Mateescu12Simona M. Coman13DDS Diagnostic, 031427 Bucharest, RomaniaDDS Diagnostic, 031427 Bucharest, RomaniaDDS Diagnostic, 031427 Bucharest, RomaniaDDS Diagnostic, 031427 Bucharest, RomaniaDepartment of Inorganic Chemistry, Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030016 Bucharest, RomaniaDepartment of Inorganic Chemistry, Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030016 Bucharest, RomaniaDepartment of Inorganic Chemistry, Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030016 Bucharest, RomaniaDepartment of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, RomaniaDepartment of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaNational Institute for Research and Development in Microtechnologies, 126 A Erou Iancu Nicolae, 077190 Voluntari City, RomaniaNational Institute for Research and Development in Microtechnologies, 126 A Erou Iancu Nicolae, 077190 Voluntari City, RomaniaDDS Diagnostic, 031427 Bucharest, RomaniaDepartment of Inorganic Chemistry, Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030016 Bucharest, RomaniaHydrogels are a viable option for biomedical applications due to their biocompatibility, biodegradability, and ability to incorporate various healing agents while maintaining their biological efficacy. This study focused on the preparation and characterization of novel hybrid hydrogels enriched with the natural algae compound Ulvan for potential use in wound dressings. The characterization of the hydrogel membranes involved multiple methods to assess their structural, mechanical, and chemical properties, such as pH measurements, swelling, moisture content and uptake, gel fraction, hydrolytic degradation, protein adsorption and denaturation tests, rheological measurements, SEM, biocompatibility testing, and scratch wound assay. The hydrogel obtained with a higher concentration of Ulvan (1 mg/mL) exhibited superior mechanical properties, a swelling index of 264%, a water content of 55%, and a lower degradation percentage. In terms of rheological properties, the inclusion of ULV in the hydrogel composition enhanced gel strength, and the Alginate + PVA + 1.0ULV sample demonstrated the greatest resistance to deformation. All hydrogels exhibited good biocompatibility, with cell viability above 70% and no obvious morphological modifications. The addition of Ulvan potentiates the regenerative effect of hydrogel membranes. Subsequent studies will focus on encapsulating bioactive compounds, investigating their release behavior, and evaluating their active biological effects.https://www.mdpi.com/2310-2861/11/1/17hydrogelwoundhealingdressingUlvanalgae |
| spellingShingle | Dana Stan Andreea-Cristina Mirica Sorin Mocanu Diana Stan Iunia Podolean Natalia Candu Magdi El Fergani Laura Mihaela Stefan Ana-Maria Seciu-Grama Ludmila Aricov Oana Brincoveanu Carmen Moldovan Lorena-Andreea Bocancia-Mateescu Simona M. Coman Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical Applications Gels hydrogel wound healing dressing Ulvan algae |
| title | Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical Applications |
| title_full | Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical Applications |
| title_fullStr | Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical Applications |
| title_full_unstemmed | Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical Applications |
| title_short | Hybrid Hydrogel Supplemented with Algal Polysaccharide for Potential Use in Biomedical Applications |
| title_sort | hybrid hydrogel supplemented with algal polysaccharide for potential use in biomedical applications |
| topic | hydrogel wound healing dressing Ulvan algae |
| url | https://www.mdpi.com/2310-2861/11/1/17 |
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