scFv Antibody: Principles and Clinical Application
To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produc...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/980250 |
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| author | Zuhaida Asra Ahmad Swee Keong Yeap Abdul Manaf Ali Wan Yong Ho Noorjahan Banu Mohamed Alitheen Muhajir Hamid |
| author_facet | Zuhaida Asra Ahmad Swee Keong Yeap Abdul Manaf Ali Wan Yong Ho Noorjahan Banu Mohamed Alitheen Muhajir Hamid |
| author_sort | Zuhaida Asra Ahmad |
| collection | DOAJ |
| description | To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase. These bifunctional proteins having both antigen-binding capacity and marker activity can be obtained from transformed bacteria and used for one-step immunodetection of biological agents. Alternatively, antibody fragments could also be applied in the construction of immunotoxins, therapeutic gene delivery, and anticancer intrabodies for therapeutic purposes. This paper provides an overview of the current studies on the principle, generation, and application of scFv. The potential of scFv in breast cancer research is also discussed in this paper. |
| format | Article |
| id | doaj-art-341ffe2a58f445e3b93d6aa49d739f40 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-341ffe2a58f445e3b93d6aa49d739f402025-02-03T07:25:18ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/980250980250scFv Antibody: Principles and Clinical ApplicationZuhaida Asra Ahmad0Swee Keong Yeap1Abdul Manaf Ali2Wan Yong Ho3Noorjahan Banu Mohamed Alitheen4Muhajir Hamid5Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, MalaysiaInstitute of Bioscience, Universiti Putra Malaysia, Selangor, 43400 Serdang, MalaysiaFaculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, MalaysiaDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, MalaysiaDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, MalaysiaDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, MalaysiaTo date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase. These bifunctional proteins having both antigen-binding capacity and marker activity can be obtained from transformed bacteria and used for one-step immunodetection of biological agents. Alternatively, antibody fragments could also be applied in the construction of immunotoxins, therapeutic gene delivery, and anticancer intrabodies for therapeutic purposes. This paper provides an overview of the current studies on the principle, generation, and application of scFv. The potential of scFv in breast cancer research is also discussed in this paper.http://dx.doi.org/10.1155/2012/980250 |
| spellingShingle | Zuhaida Asra Ahmad Swee Keong Yeap Abdul Manaf Ali Wan Yong Ho Noorjahan Banu Mohamed Alitheen Muhajir Hamid scFv Antibody: Principles and Clinical Application Clinical and Developmental Immunology |
| title | scFv Antibody: Principles and Clinical Application |
| title_full | scFv Antibody: Principles and Clinical Application |
| title_fullStr | scFv Antibody: Principles and Clinical Application |
| title_full_unstemmed | scFv Antibody: Principles and Clinical Application |
| title_short | scFv Antibody: Principles and Clinical Application |
| title_sort | scfv antibody principles and clinical application |
| url | http://dx.doi.org/10.1155/2012/980250 |
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