MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue
MicroRNAs (miRNAs) are short noncoding RNAs involved in posttranscriptional regulation of gene expression and influence many cellular functions including glucose and lipid metabolism. We previously reported that adipose tissue (AT) from women with polycystic ovary syndrome (PCOS) or controls with in...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/943659 |
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| author | Tung-Yueh Chuang Hsiao-Li Wu Chen-Chun Chen Gloria Mabel Gamboa Lawrence C. Layman Michael P. Diamond Ricardo Azziz Yen-Hao Chen |
| author_facet | Tung-Yueh Chuang Hsiao-Li Wu Chen-Chun Chen Gloria Mabel Gamboa Lawrence C. Layman Michael P. Diamond Ricardo Azziz Yen-Hao Chen |
| author_sort | Tung-Yueh Chuang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are short noncoding RNAs involved in posttranscriptional regulation of gene expression and influence many cellular functions including glucose and lipid metabolism. We previously reported that adipose tissue (AT) from women with polycystic ovary syndrome (PCOS) or controls with insulin resistance (IR) revealed a differentially expressed microRNA (miRNA) profile, including upregulated miR-93 in PCOS patients and in non-PCOS women with IR. Overexpressed miR-93 directly inhibited glucose transporter isoform 4 (GLUT4) expression, thereby influencing glucose metabolism. We have now studied the role of miR-223, which is also abnormally expressed in the AT of IR subjects. Our data indicates that miR-223 is significantly overexpressed in the AT of IR women, regardless of whether they had PCOS or not. miR-223 expression in AT was positively correlated with HOMA-IR. Unlike what is reported in cardiomyocytes, overexpression of miR-223 in human differentiated adipocytes was associated with a reduction in GLUT4 protein content and insulin-stimulated glucose uptake. In addition, our data suggests miR-223 regulates GLUT4 expression by direct binding to its 3′ untranslated region (3′UTR). In conclusion, in AT miR-223 is an IR-related miRNA that may serve as a potential therapeutic target for the treatment of IR-related disorders. |
| format | Article |
| id | doaj-art-3410a183fe1a45a9a604d2e01b407104 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-3410a183fe1a45a9a604d2e01b4071042025-02-03T01:27:50ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/943659943659MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose TissueTung-Yueh Chuang0Hsiao-Li Wu1Chen-Chun Chen2Gloria Mabel Gamboa3Lawrence C. Layman4Michael P. Diamond5Ricardo Azziz6Yen-Hao Chen7Department of Obstetrics/Gynecology, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USADepartment of Obstetrics/Gynecology, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USADepartment of Biostatistics, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USADepartment of Surgery, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USADepartment of Obstetrics/Gynecology, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USADepartment of Obstetrics/Gynecology, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USADepartment of Obstetrics/Gynecology, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USADepartment of Obstetrics/Gynecology, Georgia Regents University, 1120 15th Street, Augusta, GA 30912, USAMicroRNAs (miRNAs) are short noncoding RNAs involved in posttranscriptional regulation of gene expression and influence many cellular functions including glucose and lipid metabolism. We previously reported that adipose tissue (AT) from women with polycystic ovary syndrome (PCOS) or controls with insulin resistance (IR) revealed a differentially expressed microRNA (miRNA) profile, including upregulated miR-93 in PCOS patients and in non-PCOS women with IR. Overexpressed miR-93 directly inhibited glucose transporter isoform 4 (GLUT4) expression, thereby influencing glucose metabolism. We have now studied the role of miR-223, which is also abnormally expressed in the AT of IR subjects. Our data indicates that miR-223 is significantly overexpressed in the AT of IR women, regardless of whether they had PCOS or not. miR-223 expression in AT was positively correlated with HOMA-IR. Unlike what is reported in cardiomyocytes, overexpression of miR-223 in human differentiated adipocytes was associated with a reduction in GLUT4 protein content and insulin-stimulated glucose uptake. In addition, our data suggests miR-223 regulates GLUT4 expression by direct binding to its 3′ untranslated region (3′UTR). In conclusion, in AT miR-223 is an IR-related miRNA that may serve as a potential therapeutic target for the treatment of IR-related disorders.http://dx.doi.org/10.1155/2015/943659 |
| spellingShingle | Tung-Yueh Chuang Hsiao-Li Wu Chen-Chun Chen Gloria Mabel Gamboa Lawrence C. Layman Michael P. Diamond Ricardo Azziz Yen-Hao Chen MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue Journal of Diabetes Research |
| title | MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue |
| title_full | MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue |
| title_fullStr | MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue |
| title_full_unstemmed | MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue |
| title_short | MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue |
| title_sort | microrna 223 expression is upregulated in insulin resistant human adipose tissue |
| url | http://dx.doi.org/10.1155/2015/943659 |
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