LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae)
This study aims to evaluate the anti-inflammatory, cytotoxicity, and genotoxicity activities of Dissotis multiflora (Sm) Triana and Paullinia pinnata Linn used traditionally in Cameroon to treat infectious diseases. Phytochemical screening was carried out using the LC-MS procedure. The ferrous oxida...
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Wiley
2020-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2020/5169847 |
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| author | Alian Désiré Afagnigni Maximilienne Ascension Nyegue Steve Valdi Djova François-Xavier Etoa |
| author_facet | Alian Désiré Afagnigni Maximilienne Ascension Nyegue Steve Valdi Djova François-Xavier Etoa |
| author_sort | Alian Désiré Afagnigni |
| collection | DOAJ |
| description | This study aims to evaluate the anti-inflammatory, cytotoxicity, and genotoxicity activities of Dissotis multiflora (Sm) Triana and Paullinia pinnata Linn used traditionally in Cameroon to treat infectious diseases. Phytochemical screening was carried out using the LC-MS procedure. The ferrous oxidation-xylenol orange (FOX) assay was used to determine the 15-lipoxygenase (15-LOX) inhibitory activity of the plant samples. The tetrazolium-based colorimetric (MTT) assay was performed using Vero cells. The Ames test was carried out using Salmonella typhimurium TA98 and TA100 tester strains. LC-MS chromatogram of D. multiflora led to the identification of four known compounds, namely, 5-(3,5-dinitrophenyl)-2H-tetrazol (2), 2,2'-{[2-(6-amino-9H-purine-9-yl)ethyl]imino}diethanol (14), 1,2,5-oxadiazolo [3,4-b]pyrazine, 5,6-di (3,5-dimethyl-1-piperidyl) (19), and nimbolinin D (20) while four compounds were also identified in P. pinnata known as 2-hydroxycarbamoyl-4-methyl-pentanoic acid (2), pheophorbide A (16), 1-[4-({2-[(1-methyl-1H-indol-5-yl)amino]-4-pyrimidinyl}oxy)-1-naphthyl]-3-[1-(4 methylphenyl)-3-(2-methyl-2-propanyl)-1H-pyrazol-5-yl]urea (17), and nimbolinin D (18). D. multiflora and P. pinnata inhibited 15-LOX activity in concentration-dependent manner. The LC50 (concentration that kills 50% of cells) values of the extracts ranged from 0.13 ± 00 to 1 ± 00 mg/mL for P. pinnata and D. multiflora, respectively. P. pinnata was cytotoxic at concentrations tested while D. multiflora was not. The selectivity index (SI) values ranged from 0.16 to 10.30 on Vero cell lines. No genotoxic effect was observed against both strains tested. These extracts are sources of compounds which can be used to control infectious diseases and associated inflammation. However, caution should be taken while using P. pinnata for medicinal purposes. |
| format | Article |
| id | doaj-art-34093e0668e448efbad1cedb35d5aa7f |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | Journal of Tropical Medicine |
| spelling | doaj-art-34093e0668e448efbad1cedb35d5aa7f2025-02-03T06:04:38ZengWileyJournal of Tropical Medicine1687-96861687-96942020-01-01202010.1155/2020/51698475169847LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae)Alian Désiré Afagnigni0Maximilienne Ascension Nyegue1Steve Valdi Djova2François-Xavier Etoa3Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box: 812, Yaounde, CameroonDepartment of Microbiology, Faculty of Science, University of Yaounde I, P.O. Box: 812, Yaounde, CameroonDepartment of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box: 812, Yaounde, CameroonDepartment of Microbiology, Faculty of Science, University of Yaounde I, P.O. Box: 812, Yaounde, CameroonThis study aims to evaluate the anti-inflammatory, cytotoxicity, and genotoxicity activities of Dissotis multiflora (Sm) Triana and Paullinia pinnata Linn used traditionally in Cameroon to treat infectious diseases. Phytochemical screening was carried out using the LC-MS procedure. The ferrous oxidation-xylenol orange (FOX) assay was used to determine the 15-lipoxygenase (15-LOX) inhibitory activity of the plant samples. The tetrazolium-based colorimetric (MTT) assay was performed using Vero cells. The Ames test was carried out using Salmonella typhimurium TA98 and TA100 tester strains. LC-MS chromatogram of D. multiflora led to the identification of four known compounds, namely, 5-(3,5-dinitrophenyl)-2H-tetrazol (2), 2,2'-{[2-(6-amino-9H-purine-9-yl)ethyl]imino}diethanol (14), 1,2,5-oxadiazolo [3,4-b]pyrazine, 5,6-di (3,5-dimethyl-1-piperidyl) (19), and nimbolinin D (20) while four compounds were also identified in P. pinnata known as 2-hydroxycarbamoyl-4-methyl-pentanoic acid (2), pheophorbide A (16), 1-[4-({2-[(1-methyl-1H-indol-5-yl)amino]-4-pyrimidinyl}oxy)-1-naphthyl]-3-[1-(4 methylphenyl)-3-(2-methyl-2-propanyl)-1H-pyrazol-5-yl]urea (17), and nimbolinin D (18). D. multiflora and P. pinnata inhibited 15-LOX activity in concentration-dependent manner. The LC50 (concentration that kills 50% of cells) values of the extracts ranged from 0.13 ± 00 to 1 ± 00 mg/mL for P. pinnata and D. multiflora, respectively. P. pinnata was cytotoxic at concentrations tested while D. multiflora was not. The selectivity index (SI) values ranged from 0.16 to 10.30 on Vero cell lines. No genotoxic effect was observed against both strains tested. These extracts are sources of compounds which can be used to control infectious diseases and associated inflammation. However, caution should be taken while using P. pinnata for medicinal purposes.http://dx.doi.org/10.1155/2020/5169847 |
| spellingShingle | Alian Désiré Afagnigni Maximilienne Ascension Nyegue Steve Valdi Djova François-Xavier Etoa LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae) Journal of Tropical Medicine |
| title | LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae) |
| title_full | LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae) |
| title_fullStr | LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae) |
| title_full_unstemmed | LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae) |
| title_short | LC-MS Analysis, 15-Lipoxygenase Inhibition, Cytotoxicity, and Genotoxicity of Dissotis multiflora (Sm) Triana (Melastomataceae) and Paullinia pinnata Linn (Sapindaceae) |
| title_sort | lc ms analysis 15 lipoxygenase inhibition cytotoxicity and genotoxicity of dissotis multiflora sm triana melastomataceae and paullinia pinnata linn sapindaceae |
| url | http://dx.doi.org/10.1155/2020/5169847 |
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