Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS)
Accurate characterization of synthetic liposomes is essential since they give information about the vesicu-lar structures in bodily fluids such as extracellular vesicles. The characterization tasks are generally the determination of the sizes of the liposomes and the profiling of the liposomes'...
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Çanakkale Onsekiz Mart University
2023-12-01
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| Series: | Journal of Advanced Research in Natural and Applied Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3088152 |
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| author | Şeyma Parlatan |
| author_facet | Şeyma Parlatan |
| author_sort | Şeyma Parlatan |
| collection | DOAJ |
| description | Accurate characterization of synthetic liposomes is essential since they give information about the vesicu-lar structures in bodily fluids such as extracellular vesicles. The characterization tasks are generally the determination of the sizes of the liposomes and the profiling of the liposomes' content. Optical tweezers and Surface Enhanced Raman Spectroscopy (SERS) were used to profile the nanosized liposomes. The size distribution of the trapped liposomes (140 nm on average) was found by using Einstein's Brownian motion equation, consistent with the size distribution obtained from dynamic light scattering measure-ments. Besides, Gramicidin-encapsulated liposomes were measured using SERS, and statistically signifi-cant differentiation was found in Raman intensities between liposome populations with altering concentra-tions of proteins. This study uniquely measured size distributions of nano-sized liposomes with conven-tional optical tweezers (without plasmonics) and determined the chemical differences between empty and protein encapsulated liposomes with high accuracy using Raman spectroscopy |
| format | Article |
| id | doaj-art-33962e7d1a8a4c79afb67b67245fde49 |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-33962e7d1a8a4c79afb67b67245fde492025-02-05T17:57:35ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952023-12-019491292210.28979/jarnas.1284172453Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS)Şeyma Parlatan0https://orcid.org/0000-0003-2355-4674İSTİNYE ÜNİVERSİTESİAccurate characterization of synthetic liposomes is essential since they give information about the vesicu-lar structures in bodily fluids such as extracellular vesicles. The characterization tasks are generally the determination of the sizes of the liposomes and the profiling of the liposomes' content. Optical tweezers and Surface Enhanced Raman Spectroscopy (SERS) were used to profile the nanosized liposomes. The size distribution of the trapped liposomes (140 nm on average) was found by using Einstein's Brownian motion equation, consistent with the size distribution obtained from dynamic light scattering measure-ments. Besides, Gramicidin-encapsulated liposomes were measured using SERS, and statistically signifi-cant differentiation was found in Raman intensities between liposome populations with altering concentra-tions of proteins. This study uniquely measured size distributions of nano-sized liposomes with conven-tional optical tweezers (without plasmonics) and determined the chemical differences between empty and protein encapsulated liposomes with high accuracy using Raman spectroscopyhttps://dergipark.org.tr/en/download/article-file/3088152liposomeoptical trappingsurface enhanced raman spectroscopygramicidinprotein determination |
| spellingShingle | Şeyma Parlatan Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS) Journal of Advanced Research in Natural and Applied Sciences liposome optical trapping surface enhanced raman spectroscopy gramicidin protein determination |
| title | Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS) |
| title_full | Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS) |
| title_fullStr | Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS) |
| title_full_unstemmed | Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS) |
| title_short | Determination of Protein Amount in Nanosized Synthetic Liposomes by Surface Effect Raman Spectroscopy (SERS) |
| title_sort | determination of protein amount in nanosized synthetic liposomes by surface effect raman spectroscopy sers |
| topic | liposome optical trapping surface enhanced raman spectroscopy gramicidin protein determination |
| url | https://dergipark.org.tr/en/download/article-file/3088152 |
| work_keys_str_mv | AT seymaparlatan determinationofproteinamountinnanosizedsyntheticliposomesbysurfaceeffectramanspectroscopysers |