Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology
Objective To establish a homozygous zebrafish map3k15 knockout line using CRISPR/Cas9 gene editing technology so to provide an animal model for investigation of potential role of map3k15 in renal diseases. Methods 1)Analyzing the expression pattern of map3k15 in zebrafish; 2)Establishing a homozygou...
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| Main Author: | |
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| Format: | Article |
| Language: | zho |
| Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-03-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-3-281-290.pdf |
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| Summary: | Objective To establish a homozygous zebrafish map3k15 knockout line using CRISPR/Cas9 gene editing technology so to provide an animal model for investigation of potential role of map3k15 in renal diseases. Methods 1)Analyzing the expression pattern of map3k15 in zebrafish; 2)Establishing a homozygous map3k15 knockout zebrafish line using CRISPR/Cas9; 3)Observing the phenotypic effects of map3k15 deficiency in zebrafish. Results 1)map3k15 was expressed in the zebrafish pronephros, and map3k15/map3k15 protein was found highly conserved across species; 2)map3k15-/- mutant zebrafish model was constructed with +2 bp mutation and +1 bp mutation; 3)map3k15 mutant zebrafish exhibited edema in yolk sac, pericardium and head during embryonic development. Conclusions A homozygous zebrafish map3k15 knockout line is successfully developed which may provide an important model for future research on the role of map3k15 in renal development and disease. |
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| ISSN: | 1001-6325 |